Volume 7, Issue 1 (spring[PERSIAN] 2013)                   mljgoums 2013, 7(1): 1-6 | Back to browse issues page

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SamadiKafil H, MohebatiMobarez M, ForouzandehMoghadam M. Comparing MALDI-ToFMass Spectrometry with Molecular and Biochemical Methods in Identifying Enterococcus Faecium and Enterococcus FaecalisIsolated from Clinical Samples. mljgoums 2013; 7 (1) :1-6
URL: http://mlj.goums.ac.ir/article-1-251-en.html
Comparing MALDI-ToFMass Spectrometry with Molecular and Biochemical Methods in Identifying Enterococcus Faecium and Enterococcus FaecalisIsolated from Clinical Samples
H SamadiKafil1 , M MohebatiMobarez 2, M ForouzandehMoghadam3
1- ,Department of Medical Bacteriology, Faculty of Medical Sciences, Tarbiatmodares University
2- Department of Medical Bacteriology, Faculty of Medical Sciences, Tarbiatmodares University , mmmobarez@modares.ac.ir
3- Department of Biotechnology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
Abstract:   (20464 Views)
Abstract Background and Objective: Enterococci are Gram-positive members of human gastrointestinal flora,in Dairy products and environment. they have emerged as important causes of opportunistic nosocomial infections in recent years. In this study we aimed to investigat and compare the efficiency of MALDI-TOFmass spectroscopy method through Biochemical and Molecular methods for detecting Enterococcus faecalis and Enterococcus faecium. Materials and Methods:seventhy five clinical samples were collected for biochemical, molecular and mass spectroscopy investigations. Samples were treated with Esculin hydrolysis, Catalase, Pyrrolidonylaminopeptidase, 6.5% NaCl solution, motility, 0.04% Tellurite, L-Arabinose and Sorbitol. Using specific primesallele specific PCR was used.The samples were then analyzed by MALDI-TOF mass spectroscopy and Biotyper 3 software. Results:Enterococcus faecium andEnterococcus faecaliswere detected in thirty and forty two samples, respectively whereas three samples showed both bacterial infections. Using biochemical analysis, two E.faecium isolates were Arabinose negative and one E. faecalis isolates was Telliurite negative. All sampleswere showed correct bands in PCR results but twoof them didn't show clear bands(on agarose gel). In mass spectroscopy analysis all strains were correctly detected and well defined. Conclusion: According to our results, MALDI-TOF mass spectrometry in comparison with Molecular and Biochemical Methodscould be a reliable and accurate method that can easily and quickly identify and differentiate Enterococcus faecium and Enterococcus faecalisin clinical samples. Key words:Enterococcus faecalis, Enterococcus faecium, MALDI-TOFmass spectrometry,PCR
Keywords: Enterococcus faecalis, Enterococcus faecium, MALDI-TOFmass spectrometry, PCR
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Research Article: Original Paper |
Received: 2013/03/9 | Accepted: 2013/05/26 | Published: 2013/05/26 | ePublished: 2013/05/26
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.