Thu, Apr 25, 2024
Volume 17, Issue 2 (Mar-Apr 2023)
mljgoums 2023, 17(2): 26-32 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
rashidpour F, farzanegi P, abbaszadeh H. Evaluation of Cyclooxygenase-2 and Prostaglandin E2 Expression in Endometrial Tissue Following 8 Weeks of Swimming Exercise and Omega-3 Intake. mljgoums 2023; 17 (2) :26-32
URL: http://mlj.goums.ac.ir/article-1-1536-en.html
URL: http://mlj.goums.ac.ir/article-1-1536-en.html
1- Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran
2- Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, , parvin.farzanegi@gmil.com
2- Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, , parvin.farzanegi@gmil.com
Abstract: (650 Views)
Background and objectives: Endometriosis is a chronic disease that affects approximately 10% of women of reproductive age. The purpose of this study was to evaluate the effects of 8 weeks of swimming exercise and omega-3 supplementation on the expression of cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) genes and serum levels of reproductive hormones in the endometrial tissue of a rat model of endometriosis.
Methods: In this experimental study, 30 adult Wistar rats were randomly divided into 5 groups of healthy-control, patient-control, patient+exercise, patient+omega-3, and patient+omega-3+exercise groups. After the induction of endometriosis, the rats were subjected to 8 weeks of swimming exercise, 5 days a week as well as 2 ml/kg/body weight of omega-3. Data were analyzed using one-way analysis of variance and Tukey post hoc test at the significance level of 0.05.
Results: The expression of COX-2 and PGE2 as well as serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol levels were significantly higher in the patient group compared with the healthy-control group (p≤0.0001). Exercise and omega-3 supplementation either separately or combined could significantly reduce the expression of both genes and serum levels of LH, FSH, and estradiol (p<0.05). This effect was more profound in the patient+exercise+omega-3 group.
Conclusion: The results of the present study indicate that regulating the expression of COX-2 and PGE2 genes as well as serum levels of reproductive hormones through swimming exercise and omega-3 supplementation can improve endometriosis.
Methods: In this experimental study, 30 adult Wistar rats were randomly divided into 5 groups of healthy-control, patient-control, patient+exercise, patient+omega-3, and patient+omega-3+exercise groups. After the induction of endometriosis, the rats were subjected to 8 weeks of swimming exercise, 5 days a week as well as 2 ml/kg/body weight of omega-3. Data were analyzed using one-way analysis of variance and Tukey post hoc test at the significance level of 0.05.
Results: The expression of COX-2 and PGE2 as well as serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol levels were significantly higher in the patient group compared with the healthy-control group (p≤0.0001). Exercise and omega-3 supplementation either separately or combined could significantly reduce the expression of both genes and serum levels of LH, FSH, and estradiol (p<0.05). This effect was more profound in the patient+exercise+omega-3 group.
Conclusion: The results of the present study indicate that regulating the expression of COX-2 and PGE2 genes as well as serum levels of reproductive hormones through swimming exercise and omega-3 supplementation can improve endometriosis.
Research Article: Research Article |
Subject:
Others
Received: 2022/06/19 | Accepted: 2022/08/31 | Published: 2023/03/14 | ePublished: 2023/03/14
Received: 2022/06/19 | Accepted: 2022/08/31 | Published: 2023/03/14 | ePublished: 2023/03/14
References
1. Patel BG, Lenk EE, Lebovic DI, Shu Y, Yu J, Taylor RN. Pathogenesis of endometriosis: Interaction between Endocrine and inflammatory pathways. Gynaecol. 2018; 50: 50-60. [View at Publisher] [DOI:10.1097/01.AOG.0000546648.93614.55] [PubMed] [Google Scholar]
2. Donnez J, Dolmans M M. Endometriosis and Medical Therapy: From Progestogens to Progesterone Resistance to GnRH Antagonists: A Review. J. Clin. Med. 2021;10, 1085. [View at Publisher] [DOI:10.3390/jcm10051085] [PubMed] [Google Scholar]
3. Genovese T, Siracusa R, OrcID, D'Amico R, Cordaro M,et al. Regulation of Inflammatory and Proliferative Pathways by Fotemustine and Dexamethasone in Endometriosis. Int J Mol Sci. 2021; 22(11): 5998. [View at Publisher] [DOI:10.3390/ijms22115998] [PubMed] [Google Scholar]
4. Yan H, Zhoa H, Kang Y, Ji X, Zhang T, Wang Y, Cui R,Zhang G, Shi G.Parecoxib alleviates the motor behavioral decline of aged rats by ameliorating mitochondrial dysfunction in the substantia nigra via COX-2/PGE2 pathway inhibition. Neuropharmacology. 2021; 194(15): 108627. [View at Publisher] [DOI:10.1016/j.neuropharm.2021.108627] [PubMed] [Google Scholar]
5. Balan A, Moga MA, Dima L, Dinu CG, Martinescu CC, Panait DE, Irimie CA, Anastasiu CV. An Overview on the Conservative Management of Endometriosis from a Naturopathic Perspective: Phytochemicals and Medicinal Plants. 2021; 10:587. [View at Publisher] [DOI:10.3390/plants10030587] [PubMed] [Google Scholar]
6. Yu PH, Chou PY, Li WN , Tsai SJ,Wu MH. The pro-inflammatory and anti-inflammatory role of hyaluronic acid in endometriosis. J of Obstetrics and Gynaecology. 2021; 60: 711-717. [View at Publisher] [DOI:10.1016/j.tjog.2021.05.022] [PubMed] [Google Scholar]
7. UÇAN B, ÖZBEK M, ŞAHİN M, KIZILGÜL M, ÇAKAL E. Cyclooxygenase-2 (COX-2) gene polymorphism in patients with differentiated thyroid carcinomas in the Turkish population. Turk J Med Sci. 2017;47: 1848-1853. [View at Publisher] [DOI:10.3906/sag-1708-49] [PubMed] [Google Scholar]
8. Hansen S, Sverrisdóttir UÁ, Rudnicki M. Impact of exercise on pain perception in women with endometriosis: A systematic review. Acta Obstet Gynecol Scand. 2021; 100(9): 1595-1601. [View at Publisher] [DOI:10.1111/aogs.14169] [PubMed] [Google Scholar]
9. Shahidian Akbar F, Farzanegi P, Abbaszadeh H. Evaluation of ESR and IGFBP1 genes of ovarian tissue of endometriosis model mice after a period of regular exercise and vitamin E intake. Razi J Med Sci. 2020;27(3):38-48. [View at Publisher] [Google Scholar]
10. Montenegro ML, Bonocher CM, Meola J, Portella RL, Ribeiro-Silva A, Brunaldi MO, et al. Effect of Physical Exercise on Endometriosis Experimentally Induced in Rats. Reprod Sci. 2019; 26(6): 785-793. [View at Publisher] [DOI:10.1177/1933719118799205] [PubMed] [Google Scholar]
11. Lee YY, Yang YP ,Huang PI, et al. Exercise suppresses COX-2 pro-inflammatory pathway in vestibular migraine. 2015;116:98-105. [View at Publisher] [DOI:10.1016/j.brainresbull.2015.06.005] [PubMed] [Google Scholar]
12. Abokhrais IM, Denison FC, Whitaker LH, Saunders PT, Doust A, Williams LJ, Horne AW. A two-arm parallel double-blind randomised controlled pilot trial of the efficacy of Omega-3 polyunsaturated fatty acids for the treatment of women with endometriosis-associated pain (PurFECT1). PloS one. 2020;15(1):e0227695. [View at Publisher] [DOI:10.1371/journal.pone.0227695] [PubMed] [Google Scholar]
13. Kiani K, Movahedin M, Malekafzali H, Mirfasihi F, Nargess Sadati S.N, Moini A, et al. Effect of the estrus cycle stage on the establishment of murine endometriosis lesions. Int J Reprod Biomed (Yazd). 2018; 16(5): 305-314. [View at Publisher] [DOI:10.29252/ijrm.16.5.305]
14. Vallée A, Vallée JN, Le Blanche A, Lecarpentier Y. PPARγ Agonists: Emergent Therapy in Endometriosis. Pharmaceuticals (Basel). 2021;14(6):543. [View at Publisher] [DOI:10.3390/ph14060543] [PubMed] [Google Scholar]
15. Dai S , Zhu M, Wu R, Lin D, Huang Z, Ren L, Huang S, Cheng L, Chen Q. Lipoxin A4 Suppresses IL-1b-Induced Cyclooxygenase-2 Expression Through Inhibition of p38 MAPK Activation in Endometriosis. Reproductive Sciences. 2019; 26(12) :1640-1649. [View at Publisher] [DOI:10.1177/1933719119828115] [PubMed] [Google Scholar]
16. Bulun SE, Monsavais D, Pavone ME, Dyson M, Xue Q, Attar E, Tokunaga H, Su EJ. Role of estrogen receptor-β in endometriosis. Semin Reprod Med. 2012; 30(1): 39-45. [View at Publisher] [DOI:10.1055/s-0031-1299596] [PubMed] [Google Scholar]
17. Cai M, Ni WJ, Han L, Chen WD, Peng DY.Research Progress of Therapeutic Enzymes and Their Derivatives: Based on Herbal Medicinal Products in Rheumatoid Arthritis. 2021; 12: 626342. [View at Publisher] [DOI:10.3389/fphar.2021.626342] [PubMed] [Google Scholar]
18. Arosh JA, Lee J, Balasubbramanian D, Jone A, et al. Molecular and preclinical basis to inhibit PGE2 receptors EP2 and EP4 as a novel nonsteroidal therapy for endometriosis. 2015;112 (31): 9716-9721. [View at Publisher] [DOI:10.1073/pnas.1507931112] [PubMed] [Google Scholar]
19. Cho S, Park SH, Choi YS, Seo SK, Kim HY, Park KH, et al. Expression of Cyclooxygenase-2 in Eutopic Endometrium and Ovarian Endometriotic Tissue in Women with Severe Endometriosis. Gynecol Obstet Investig. 2010; 69: 93-100. [View at Publisher] [DOI:10.1159/000261017] [PubMed] [Google Scholar]
20. Ming-jiang Li , Ji-jun Li, Xing-bo Zhao, Mei-juan Zhang, Wei Wang .Influence of mifepristone and lilopristone on proliferation and expression of nuclear factor-kappa B of ectopic stromal cells in vitro. Zhonghua Fu Chan Ke Za Zhi . 2005;40(1):21-4. [PubMed] [Google Scholar]
21. Ghasemian Langharodi S, Farzanegi P, Moradi L. The Effect of Swimming Training and Vitamin B6 Intake on GATA2 Gene Expression in Endometriosis Rat. 2019; 19(3): 354-365. [View at Publisher] [DOI:10.29252/jarums.19.3.354] [Google Scholar]
22. Trappe TA, Standley RA, Jemiolo B, Carroll CC, Trappe SW. Prostaglandin and myokine involvement in the cyclooxygenase-inhibiting drug enhancement of skeletal muscle adaptations to resistance exercise in older adults. 2013;304(3): 198-205. [View at Publisher] [DOI:10.1152/ajpregu.00245.2012] [PubMed] [Google Scholar]
23. Attaman JA, Stanic AK, Kim M, Lynch MP, Rueda BR, Styer AK. The anti‐inflammatory impact of omega‐3 polyunsaturated Fatty acids during the establishment of endometriosis‐like lesions. American Journal of Reproductive Immunology. 2014;72(4):392-402. [View at Publisher] [DOI:10.1111/aji.12276] [PubMed] [Google Scholar]
24. Capó X, Martorell M, Sureda A, Tur JA,Pons A .Effects of dietary Docosahexaenoic, training and acute exercise on lipid mediators. 2016;13:16:1-12. [View at Publisher] [DOI:10.1186/s12970-016-0126-y] [PubMed] [Google Scholar]
25. Simopoulos, AP. Omega-3 fatty acids in inflammation and autoimmune diseases. Journal of the American College of nutrition. 2002; 21(6):495-505. [View at Publisher] [DOI:10.1080/07315724.2002.10719248] [Google Scholar]
26. Calder PC. n− 3 Polyunsaturated fatty acids, inflammation, and inflammatory diseases. The American journal of clinical nutrition.2006;83(6):1505-19. [View at Publisher] [DOI:10.1093/ajcn/83.6.1505S] [Google Scholar]
27. Anderson SD, Daviskas E. The mechanism of exercisenduced asthma is... . J Allergy Clin Immunol. 2000; 106(3):453-9. [View at Publisher] [DOI:10.1067/mai.2000.109822] [PubMed] [Google Scholar]
28. Choobine C, Akbarnejad A. Borjian M, Kordi MR. The Effect of Omega-3 Supplementation on Serum Prostaglandin E2 in Athlete Women after a Single Bout of Exhaustive Exercise. Sport Biosciences. 2012; 15(4): 121-32. [Persian] [View at Publisher] [Google Scholar]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Enamad
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.