The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018

Naghizadeh, Mohammad Sadegh; Naseri, Mohsen; Anani Sarab, Gholamreza; Derakhshani, Afshin; Fereidouni, Mohammad

Volume 17, Issue 6 (Nov-Dec 2023) mljgoums 2023, 17(6): 13-15 | Back to browse issues page

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Naghizadeh M S, Naseri M, Anani Sarab G, Derakhshani A, Fereidouni M. The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018. mljgoums 2023; 17 (6) :13-15
URL: http://mlj.goums.ac.ir/article-1-1382-en.html

The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018

Mohammad Sadegh Naghizadeh¹

, Mohsen Naseri²

, Gholamreza Anani Sarab²

, Afshin Derakhshani¹

, Mohammad Fereidouni

1- . Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
2- Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
3- Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran , msnaghizadeh92@bums,ac,ir

Abstract: (334 Views)

Background: Atopic dermatitis (AD) is a common allergic disorder. Detection of responsible pathogenic allergens in AD patients by reliable methods has a fundamental role in the prevention, management, and treatment of AD. This study was conducted to determine the most common allergens by the skin prick test (SPT) and immunoblotting among AD patients referring to an allergy clinic in Birjand City, Iran.
Methods: The presence of AD was confirmed by an expert allergist. Serum levels of total and specific immunoglobulin E (sIgE) against 30 food and inhalant allergens were evaluated by a commercial immunoblotting kit (AlleisaScreen).
Results: The skin prick test was performed by a battery of 17 allergens. In total, 34 AD patients (mean age, 28.76 ± 17.36 years; range, 1-60 years; F/M ratio: 0.88) were enrolled in this study. The sensitization rates to at least 1 fungus, pollen, food, or indoor allergen by the immunoblotting method were 32.35%, 61.76%, 52.94%, and 47.05%, respectively. The most prevalent allergens were ragweed (52.94), Olive tree (41.16), Eucalyptus (35.29), date palm (35.29), and grass mix (32.28).
Conclusion: The study found that 85.29% of the studied population were sensitized to at least 1 allergen. Pollens and date palms were the most common allergens among AD patients, but the pattern of sensitization in SPT and immunoblotting was not exactly similar. Detection of allergens to which patients are sensitized and avoidance can help in the management of the disease and its symptoms.

Keywords: Allergens, Atopic dermatitis, Receptors IgE, Immunoblotting, Skin prick test (Skin Tests)