Volume 12, Issue 1 (Jan-Feb 2018)                   mljgoums 2018, 12(1): 11-16 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Taghipoor Asramy A, Ghanbari-Niaki A, Hakemi S, Naghizadeh Qomi M, Moghanny Bashi M M. Effect of 12 Weeks of Intense Endurance Training and Bee Pollen Consumption on ABCA1 Gene Expression in Small Intestine, Liver and Gastrocnemius Muscle of Male Rats. mljgoums 2018; 12 (1) :11-16
URL: http://mlj.goums.ac.ir/article-1-1045-en.html
1- Department of Biochemistry, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Mazandaran, Iran
2- Department of Exercise Biochemistry, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Mazandaran, Iran , ghanbara@umz.ac.ir
3- Department of Clinical Biochemistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Department of Statistics, Faculty of Mathematics, University of Mazandaran, Mazandaran, Iran
5- Department of Genetics, Faculty of Medicine, Kazeroon Branch, Islamic Azad University, Kazeroon, Iran
Abstract:   (12634 Views)
ABSTRACT
          Background and Objectives: The aim of this study was to evaluate the effect of 12 weeks of intense endurance training and bee pollen consumption on ABCA1 gene expression in small intestine, liver and gastrocnemius muscle tissues of male rats.
           Methods: In this study, 24 male Wistar rats (aged 6-8 weeks and weighing 90-110 g) were randomly divided into four groups of saline-control (n=6), saline-training (n=6), bee pollen-control (n=6) and bee pollen-training (n=6). The training groups exercised on a treadmill for 12 weeks (30 m/min, 90 min/day, five days/week). The bee pollen groups were given bee pollen orally (500 mg/Kg) for 12 weeks. Data were analyzed using two-way ANOVA at significance level of 0.05.
          Results: ABCA1 gene expression was highest in the liver, gastrocnemius muscle and small intestine, respectively. The findings also revealed that the intense endurance training caused a non-significant increase in ABCA1 gene expression in the small intestine and liver. However, the training caused a non-significant decrease in ABCA1 gene expression in the gastrocnemius muscle. In addition, consumption of bee pollen significantly increased ABCA1 gene expression in the small intestine and gastrocnemius muscle of male rats. However, the effect of bee pollen on the gene’s expression in the liver was not statistically significant.
           Conclusion: Based on our findings, it can be concluded that consumption of bee pollen has more beneficial effects on the ABCA1 gene expression and reverse cholesterol transport compared with the intense endurance training.
           Keywords: ABCA1 protein, Pollen, exercise.
Full-Text [PDF 676 kb]   (1711 Downloads)    
Research Article: Original Paper |
Received: 2018/02/24 | Accepted: 2018/02/24 | Published: 2018/02/24 | ePublished: 2018/02/24

References
1. Hattori H, Kujiraoka T, Egashira T, Saito E, Fujioka T, Takahashi S, et al. Association of coronary heart disease with pre-β-HDL concentrations in Japanese men. Clinical chemistry. 2004;50(3):589-95. DOI:10.1373/clinchem.2003.029207. [DOI:10.1373/clinchem.2003.029207]
2. Drobnik W, Lindenthal B, Lieser B, Ritter M, Weber TC, Liebisch G, et al. ATP-binding cassette transporter A1 (ABCA1) affects total body sterol metabolism. Gastroenterology. 2001; 120(5):1203-11. [DOI:10.1053/gast.2001.23250]
3. Oram JF. HDL apolipoproteins and ABCA1 partners in the removal of excess cellular cholesterol. Arteriosclerosis, thrombosis, and vascular biology. 2003; 23(5): 720-7. [DOI:10.1161/01.ATV.0000054662.44688.9A]
4. Lewis GF, Rader DJ. New insights into the regulation of HDL metabolism and reverse cholesterol transport. Circulation research. 2005; 96(12): 1221-32. [DOI:10.1161/01.RES.0000170946.56981.5c]
5. Wang N, Silver DL, Thiele C, Tall AR. ATP-binding cassette transporter A1 (ABCA1) functions as a cholesterol efflux regulatory protein. Journal of Biological Chemistry. 2001; 276(26): 23742-7. [DOI:10.1074/jbc.M102348200]
6. Knight B. ATP-binding cassette transporter A1: regulation of cholesterol efflux. Biochemical Society Transactions. 2004; 32(1): 124-7. [DOI:10.1042/bst0320124]
7. Yancey PG, Bortnick AE, Kellner-Weibel G, de la Llera-Moya M, Phillips MC, Rothblat GH. Importance of different pathways of cellular cholesterol efflux. Arteriosclerosis, thrombosis, and vascular biology. 2003; 23(5): 712-9. [DOI:10.1161/01.ATV.0000057572.97137.DD]
8. Durstine JL, Grandjean PW, Davis PG, Ferguson MA, Alderson NL, DuBose KD. Blood lipid and lipoprotein adaptations to exercise. Sports Medicine. 2001; 31(15): 1033-62. [DOI:10.2165/00007256-200131150-00002]
9. Brites F, Verona J, De Geitere C, Fruchart J-C, Castro G, Wikinski R. Enhanced cholesterol efflux promotion in well-trained soccer players. Metabolism. 2004; 53(10): 1262-7. [DOI:10.1016/j.metabol.2004.05.002]
10. Olchawa B, Kingwell BA, Hoang A, Schneider L, Miyazaki O, Nestel P, et al. Physical fitness and reverse cholesterol transport. Arteriosclerosis, thrombosis, and vascular biology. 2004; 24(6): 1087-91. [DOI:10.1161/01.ATV.0000128124.72935.0f]
11. Wilund KR, Colvin PL, Phares D, Goldberg AP, Hagberg JM. The effect of endurance exercise training on plasma lipoprotein AI and lipoprotein AI: AII concentrations in sedentary adults. Metabolism. 2002; 51(8): 1053-60. [DOI:10.1053/meta.2002.33356]
12. Jafari M, Leaf DA, MacRae H, Kasem J, O'Conner P, Pullinger C, et al. The effects of physical exercise on plasma prebeta-1 high-density lipoprotein. Metabolism. 2003; 52(4): 437-42. DOI:10.1053/meta.2003.50086. [DOI:10.1053/meta.2003.50086]
13. Sviridov D, Kingwell B, Hoang A, Dart A, Nestel P. Single session exercise stimulates formation of preβ1-HDL in leg muscle. Journal of lipid research. 2003; 44(3): 522-6. doi: 10.1194/jlr.M200436-JLR200. [DOI:10.1194/jlr.M200436-JLR200]
14. KILINÇ C, YAĞMUR Z, YILMAZ K, KISA Ü. The Effects of exercise and smoking on serum lecithin: cholesterol acyltransferase activity in young men. Turkish Journal of Medical Sciences. 2000; 30(2): 161-6.
15. Tsopanakis C, Kotsarellis D, Tsopanakis A. Plasma lecithin: cholesterol acyltransferase activity in elite athletes from selected sports. European journal of applied physiology and occupational physiology. 1988;58(3):262-5. [DOI:10.1007/BF00417260]
16. Ghanbari-Niaki A, Khabazian BM, Hossaini-Kakhak SA, Rahbarizadeh F, Hedayati M. Treadmill exercise enhances ABCA1 expression in rat liver. Biochemical and biophysical research communications. 2007; 361(4): 841-6. [DOI:10.1016/j.bbrc.2007.07.100]
17. Khabazian BM, Ghanbari-Niaki A, reza Safarzadeh-Golpordesari A, Ebrahimi M, Rahbarizadeh F, Abednazari H. Endurance training enhances ABCA1 expression in rat small intestine. European journal of applied physiology. 2009; 107(3): 351-8. [DOI:10.1007/s00421-009-1133-3]
18. Ghanbari-Niaki A. Treadmill exercise training enhances ATP-binding cassette protein-A1 (ABCA1) expression in male rats' heart and gastrocnemius muscles. Int J Endocrinol Metab. 2010; 8(4): 206-10.
19. Wellington CL, Walker EK, Suarez A, Kwok A, Bissada N, Singaraja R, et al. ABCA1 mRNA and protein distribution patterns predict multiple different roles and levels of regulation. Laboratory investigation. 2002; 82(3): 273-83. [DOI:10.1038/labinvest.3780421]
20. ZENG Z-j, WANG L-gRB, FAN Z-b, XIE G-x. A Study on the Effect of Honeybee Pollen Polysaccharide on Decreasing Lipoidemia in Rat [J]. Acta Agriculturae Universitis Jiangxiensis. 2004; 3: 021.
21. Selmanoğlu G, Hayretdağ S, Kolankaya D, Özkök-Tüylü A, Sorkun K. The effect of pollen on some reproductive parameters of male rats. Pesticidi i fitomedicina. 2009; 24(1): 59-63. [DOI:10.2298/PIF0901059S]
22. Kolankaya D, Şentürkb H, Tüylü AÖ, Hayretdağ S, Selmanoğlua G, Sorkun K. The effect of Trifolium, Raphanus, and Cistus pollen grains on some blood parameters and mesentery mast cells. Zeitschrift für Naturforschung C. 2006; 61(5-6): 421-6. [DOI:10.1515/znc-2006-5-619]
23. Yildiz O, Karahalil F, Can Z, Sahin H, Kolayli S. Total monoamine oxidase (MAO) inhibition by chestnut honey, pollen and propolis. Journal of enzyme inhibition and medicinal chemistry. 2014; 29(5): 690-4. doi: 10.3109/14756366.2013.843171. [DOI:10.3109/14756366.2013.843171]
24. Selmanoğlu G, Hayretdağ S, Kolankaya D, Tüylü ÖA, Sorkun K. The effects of pollen on serum parameters, and liver and kidney tissues of rats. Pesticidi i fitomedicina. 2007; 22(1): 59-64.
25. Salles J, Cardinault N, Patrac V, Berry A, Giraudet C, Collin M-L, et al. Bee Pollen Improves Muscle Protein and Energy Metabolism in Malnourished Old Rats through Interfering with the Mtor Signaling Pathway and Mitochondrial Activity. Nutrients. 2014; 6(12): 5500-16. doi: 10.3390/nu6125500. [DOI:10.3390/nu6125500]
26. Fathi R, Ghanbari-Niaki A, Rahbarizadeh F, Hedayati M, Ghahramanloo E, Farshidi Z. The effect of exercise on plasma acylated ghrelin concentrations and gastrocnemius muscle mRNA expression in male rats. Iranian Journal of Endocrinology and Metabolism. 2009; 10(5): 519-26.
27. Farke C, Viturro E, Meyer H, Albrecht C. Identification of the bovine cholesterol efflux regulatory protein ABCA1 and its expression in various tissues. Journal of animal science. 2006; 84(11): 2887-94. DOI:10.2527/jas.2006-042. [DOI:10.2527/jas.2006-042]
28. Brunham LR, Kruit JK, Iqbal J, Fievet C, Timmins JM, Pape TD, et al. Intestinal ABCA1 directly contributes to HDL biogenesis in vivo. The Journal of clinical investigation. 2006; 116(4): 1052-62. [DOI:10.1172/JCI27352]
29. Mulligan JD, Flowers MT, Tebon A, Bitgood JJ, Wellington C, Hayden MR, et al. ABCA1 is essential for efficient basolateral cholesterol efflux during the absorption of dietary cholesterol in chickens. Journal of Biological Chemistry. 2003; 278(15): 13356-66. [DOI:10.1074/jbc.M212377200]
30. Kookandeh NZ, Deldar H, Niaki AG, Pirsaraei ZA, Ahmadabad SR, Raoof Z. ABCG1 gene responses to treadmill running with or without pistachio-atlantica in female rats. Iranian Journal of Health and Physical Activity. 2012; 3(1): 1-7.
31. Vosper H. Niacin: a re‐emerging pharmaceutical for the treatment of dyslipidaemia. British journal of pharmacology. 2009;158(2): 429-41. doi: 10.1111/j.1476-5381.2009.00349.x. [DOI:10.1111/j.1476-5381.2009.00349.x]
32. Ghanbari-Niaki A, Ghanbari-Abarghooi S, Rahbarizadeh F, Zare-Kookandeh N, Gholizadeh M, Roudbari F, et al. Heart ABCA1 and PPAR-α Genes Expression Responses in Male rats: Effects of High Intensity Treadmill Running Training and Aqueous Extraction of Black Crataegus-Pentaegyna. Research in Cardiovascular Medicine. 2013; 2(4): 153-9. doi: 10.5812/cardiovascmed.13892. [DOI:10.5812/cardiovascmed.13892]
33. Lehti M, Donelan E, Abplanalp W, Al-Massadi O, Habegger K, Weber J, et al. High-density lipoprotein maintains skeletal muscle function by modulating cellular respiration in mice. Circulation. 2013; 128(22): 2364-71. doi: 10.1161/CIRCULATIONAHA.113.001551. [DOI:10.1161/CIRCULATIONAHA.113.001551]
34. Feng X-T, Wang T-Z, Chen Y, Liu J-B, Liu Y, Wang W-J. Pollen Typhae total flavone improves insulin-induced glucose uptake through the β-arrestin-2-mediated signaling in C2C12 myotubes. International journal of molecular medicine. 2012; 30(4): 914-22. doi: 10.3892/ijmm.2012.1061. [DOI:10.3892/ijmm.2012.1061]
35. Biolo G, Fleming RD, Wolfe R. Physiologic hyperinsulinemia stimulates protein synthesis and enhances transport of selected amino acids in human skeletal muscle. Journal of Clinical investigation. 1995; 95(2): 811-819. doi: 10.1172/JCI117731. [DOI:10.1172/JCI117731]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.