Volume 10, Issue 3 (10-2022)                   Jorjani Biomed J 2022, 10(3): 43-50 | Back to browse issues page

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Ramedani A, Simchi A, Sabzevari O. Fluorescent Contrast agent Based on Graphene Quantum Dots Decorated Mesoporous Silica Nanoparticles for Detecting and Sorting Cancer Cells. Jorjani Biomed J 2022; 10 (3) :43-50
URL: http://goums.ac.ir/jorjanijournal/article-1-921-en.html
1- Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran, Iran
2- Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran, Iran/ Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran , simchi@sharif.edu
3- Toxicology and Poisoning Research Centre, Tehran University of Medical Sciences, Tehran, Iran/ Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (522 Views)
Background and Objectives: The inability of classic fluorescence-activated cell sorting to single cancer cell sorting is one of the most significant drawbacks of this method. The sorting of cancer cells in microdroplets significantly influences our ability to analyze cancer cell proteins.
Material and Methods: We adapted a developed microfluidic device as a 3D in vitro model to sorted MCF-7 cancer cells on a chip. A prefabricated microfluidic droplet chip was used in this research. Then, with the help of synthesized fluorescent probes, MCF-7 cancer cells were separated from normal cells.
Results: This research presents a modification of GQD bead for high-throughput analysis and sorting single cancer cells. We elaborate a binding assay as an example of this approach for detecting MCF-7 cancer cell lines. Graphene quantum dot-decorated mesoporous silica nanoparticles (GQD@MSNPs) act as fluorescent optical beads coated in microfluidic droplets. The fluorescent beads capture cancer cells. To enable droplet sorting at 200 Hz and cell enrichment, a measurable fluorescence signal is generated when cancer cells bind to these beads and boost the drop's fluorescence emission.
Conclusion: Herein, we report in vitro results showing that the as-prepared GQD@MSNs have exceptional luminous characteristics. The specific surface area and pore volume of GQD-MSNs were found to be 50% and 40% higher than those of pure MSNs, which is rather remarkable. Because of these improved qualities, GQD@MSNs are demonstrated a large sorting capacity that makes them ideal for diagnosis.
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Type of Article: Original article | Subject: Molecular Sciences
Received: 2022/08/3 | Accepted: 2022/08/9 | Published: 2022/08/23

1. Guo MT, Rotem A, Heyman JA, Weitz DA. Droplet microfluidics for high-throughput biological assays. Lab Chip [Internet]. 2012;12(12):2146-55. [DOI] [PMID] [Google Scholar]
2. Kintses B, van Vliet LD, Devenish SRA, Hollfelder F. Microfluidic droplets: new integrated workflows for biological experiments. Curr Opin Chem Biol [Internet]. 2010;14(5):548-55. [DOI] [PMID] [Google Scholar]
3. Dove A. Drug screening-beyond the bottleneck. Nat Biotechnol [Internet]. 1999;17(9):859-63. [view at publisher] [DOI] [PMID] [Google Scholar]
4. Popat A, Hartono SB, Stahr F, Liu J, Qiao SZ, Qing Max Lu G. Mesoporous silica nanoparticles for bioadsorption, enzyme immobilisation, and delivery carriers. Nanoscale. 2011 Jul;3(7):2801-18. [DOI] [PMID] [Google Scholar]
5. He Q, Shi J. Mesoporous silica nanoparticle based nano drug delivery systems: synthesis, controlled drug release and delivery, pharmacokinetics and biocompatibility. J Mater Chem [Internet]. 2011;21(16):5845-55. [DOI] [Google Scholar]
6. Popat A, Ross BP, Liu J, Jambhrunkar S, Kleitz F, Qiao SZ. Enzyme-Responsive Controlled Release of Covalently Bound Prodrug from Functional Mesoporous Silica Nanospheres. Angew Chemie Int Ed [Internet]. 2012 Dec 7;51(50):12486-9. [DOI] [PMID] [Google Scholar]
7. Liu J, Xiong R, Brans T, Lippens S, Parthoens E, Zanacchi FC, et al. Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy. Light Sci Appl [Internet]. 2018;7(1):47. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
8. Tarn D, Ashley CE, Xue M, Carnes EC, Zink JI, Brinker CJ. Mesoporous silica nanoparticle nanocarriers: biofunctionality and biocompatibility. Acc Chem Res. 2013 Mar;46(3):792-801. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
9. Lee JE, Lee N, Kim H, Kim J, Choi SH, Kim JH, et al. Uniform mesoporous dye-doped silica nanoparticles decorated with multiple magnetite nanocrystals for simultaneous enhanced magnetic resonance imaging, fluorescence imaging, and drug delivery. J Am Chem Soc. 2010 Jan;132(2):552-7. [view at publisher] [DOI] [PMID] [Google Scholar]
10. Radu DR, Lai C-Y, Jeftinija K, Rowe EW, Jeftinija S, Lin VS-Y. A polyamidoamine dendrimer-capped mesoporous silica nanosphere-based gene transfection reagent. J Am Chem Soc. 2004 Oct;126(41):13216-7. [DOI] [PMID] [Google Scholar]
11. Slowing II, Trewyn BG, Giri S, Lin VS-Y. Mesoporous Silica Nanoparticles for Drug Delivery and Biosensing Applications. Adv Funct Mater [Internet]. 2007 May 21;17(8):1225-36. [DOI] [Google Scholar]
12. Wang Y, Li Z, Hu D, Lin C-T, Li J, Lin Y. Aptamer/graphene oxide nanocomplex for in situ molecular probing in living cells. J Am Chem Soc. 2010 Jul;132(27):9274-6. [DOI] [PMID] [Google Scholar]
13. Huang S, Song L, Xiao Z, Hu Y, Peng M, Li J, et al. Graphene quantum dot-decorated mesoporous silica nanoparticles for high aspirin loading capacity and its pH-triggered release. Anal Methods [Internet]. 2016;8(12):2561-7. [DOI] [Google Scholar]
14. Shen J, Zhu Y, Yang X, Li C. Graphene quantum dots: emergent nanolights for bioimaging, sensors, catalysis and photovoltaic devices. Chem Commun [Internet]. 2012;48(31):3686-99. [DOI] [PMID] [Google Scholar]
15. Suzuki K, Yamato K, Sekiya R, Haino T. Photoluminescence responses of graphene quantum dots toward organic bases and an acid. Photochem Photobiol Sci [Internet]. 2017;16(5):623-6. [DOI] [PMID] [Google Scholar]
16. Liang Z, Khawar MB, Liang J, Sun H. Bio-Conjugated Quantum Dots for Cancer Research: Detection and Imaging. Front Oncol. 2021;11:749970. [DOI] [PMID] [PMCID] [Google Scholar]
17. Mazutis L, Gilbert J, Ung WL, Weitz DA, Griffiths AD, Heyman JA. Single-cell analysis and sorting using droplet-based microfluidics. Nat Protoc. 2013 May 4;8(5):870-91. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]

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