Volume 10, Issue 4 (12-2022)                   Jorjani Biomed J 2022, 10(4): 1-11 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Abdolhamid Tehrani M. Study the Effect of Swimming Training and Curcumin on Reduction of BAX and P53 proteins and Increase in BCL2 protein in Heart Tissue of Rats During Withdrawal of Excessive Ethanol Consumption. Jorjani Biomed J 2022; 10 (4) :1-11
URL: http://goums.ac.ir/jorjanijournal/article-1-901-en.html
Department of Physical Education, Omidiyeh Branch, Islamic Azad University, Omidiyeh, Iran , tehrani@iauo.ac.ir
Abstract:   (305 Views)
Background and objectives: Epidemiological studies have shown that high dose of ethanol can lead to apoptosis. On the other hand, the consumption of medicinal plants such as turmeric in the diet and performing physical activities are considered as factors to control apoptosis. Therefore, this article aims to mainly evaluate the anti-apoptotic effects of swimming and curcumin interactively during withdrawal of binge ethanol exposure in heart tissues.
Material and Methods: In this study, 40 rats were received ethanol (25% W/V) as gavage for four days every eight hours. Then, 7 days after ethanol withdrawal, they were classified in 5 groups, including: (1) control, (2) curcumin, (3) swimming, (4) swimming with curcumin, and (5) sham. Groups 3 and 4 performed swimming five sessions a week for two weeks, and groups 2 and 4 received 50 mg/kg of curcumin five times a week for two weeks. The amount of BAX, BCL2, and P53 protein and BCL2/BAX ratio were measured using western blot technique. The data were tested using the independent t-test at a significant level (p≥0.05) and two-way ANOVA.
Results: Swimming had a significant effect on reduction of p53 (F=60.051, P<0.0001, η2=0.741), Bax (F=62.594, P<0.0001, η2=0.887) and Bax/Bcl-2 ratio (F=290.591, P<0.0001, η2=0.973), and increase of Bcl-2 (F=150.940, P<0.0001, η2=0.950); Curcumin had a significant effect on decrease of p53 (F=5.513, P=0.029, η2=0.208), Bax (F=66.146, P<0.0001, η2=0.892) and Bax/Bcl-2 ratio (F=260.655, P<0.0001, η2=0.970), and increase of Bcl-2 (F=73.274, P<0.0001, η2=0.902). The results from two-way ANOVA showed the interactive effect of exercise and curcumin on the decrease of BAX (F=35.847, P<0.0001, η2=0.818) and BAX/BCL-2 ratio (F=175.887, P<0.0001, η2=0.956), and the increase of BCL-2 (F=21.205, P=0.002, η2=0.726) were significant; but it had no significant effect on p53 (F=0.000, P=0.999, η2=0.000).
Conclusion: Swimming and curcumin consumption simultaneously can significantly moderate apoptosis caused by ethanol in the heart tissue.
Keywords: Curcumin [MeSH], Ethanol [MeSH], Apoptosis [MeSH]
Full-Text [PDF 571 kb]   (110 Downloads) |   |   Full-Text (HTML)  (81 Views)  
Type of Article: Original article | Subject: Health
Received: 2022/05/4 | Accepted: 2022/10/3 | Published: 2022/11/8

References
1. Mendis S, Puska P, Norrving B, World Health Organization. Global atlas on cardiovascular disease prevention and control. World Health Organization; 2011. [Google Scholar]
2. Rahmani A, Sayehmiri K, Asadollahi K, Sarokhani D, Islami F, Sarokhani M. Investigation of the prevalence of obesity in Iran: a systematic review and meta-analysis study. 2015; 596-607 [Google Scholar]
3. Jänkälä H, Eriksson CP, Eklund KK, Härkönen M, Mäki T. Combined calcium carbimide and ethanol treatment induces high blood acetaldehyde levels, myocardial apoptosis and altered expression of apoptosis-regulating genes in rat. Alcohol and Alcoholism. 2002 May 1;37(3):222-8. [DOI] [PMID] [Google Scholar]
4. Kavazis, A.N., Exercise preconditioning of the myocardium. Sports Medicine, 2009. 39(11): p. 923-935. [DOI] [PMID] [Google Scholar]
5. Fernández-Solà J, Fatjó F, Sacanella E, Estruch R, Bosch X, Urbano-Márquez A, Nicolás JM. Evidence of apoptosis in alcoholic cardiomyopathy. Human pathology. 2006 Aug 1;37(8):1100-10. [DOI] [PMID] [Google Scholar]
6. FernÁndez-SolÀ J, NicolÁs JM, FatjÓ F, GarcÍa G, Sacanella E, Estruch R, TobÍas E, Badia E, Urbano-MÁrquez A. Evidence of apoptosis in chronic alcoholic skeletal myopathy. Human pathology. 2003; 34(12):1247-52. [DOI] [PMID] [Google Scholar]
7. Fernandez-Sola J, Estruch R, Grau JM, Pare JC, Rubin E, Urbano-Marquez A. The Relation of Alcoholic Myopathy to Cardiomyopathy. Journal of Occupational and Environmental Medicine. 1995 Apr 1;37(4):419. [view at publisher] [DOI] [PMID] [Google Scholar]
8. Rodriguez A, Chawla K, Umoh NA, Cousins VM, Ketegou A, Reddy MG, AlRubaiee M, Haddad GE, Burke MW. Alcohol and apoptosis: friends or foes?. Biomolecules. 2015 Nov 19;5(4):3193-203. [DOI] [PMID] [PMCID] [Google Scholar]
9. Montazeri, F., S. Rahgozar, and K. Ghaedi, Apoptosis and cytosolic organelles. 2011. [Google Scholar]
10. Oztasan N, Taysi S, Gumustekin K, Altinkaynak K, Aktas O, Timur H, Siktar E, Keles S, Akar S, Akcay F, Dane S. Endurance training attenuates exercise-induced oxidative stress in erythrocytes in rat. European journal of applied physiology. 2004; 91(5):622-7. [DOI] [PMID] [Google Scholar]
11. Jing L, Zhou LJ, Zhang FM, Li WM, Sang Y. Tenascin-x facilitates myocardial fibrosis and cardiac remodeling through transforming growth factor-β1 and peroxisome proliferator-activated receptor γ in alcoholic cardiomyopathy. Chinese medical journal, 2011; 124(03): p. 390-395. [Google Scholar]
12. Peterson JM, Bryner RW, Sindler A, Frisbee JC, Alway SE. Mitochondrial apoptotic signaling is elevated in cardiac but not skeletal muscle in the obese Zucker rat and is reduced with aerobic exercise. Journal of applied physiology. 2008 Dec;105(6):1934-43. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
13. Lee SD, Shyu WC, Cheng IS, Kuo CH, Chan YS, Lin YM, Tasi CY, Tsai CH, Ho TJ, Huang CY. Effects of exercise training on cardiac apoptosis in obese rats. Nutrition, Metabolism and Cardiovascular Diseases. 2013; 23(6):566-73. [DOI] [PMID] [Google Scholar]
14. Jänkälä H, Eriksson PC, Eklund K, Sarviharju M, Härkönen M, Mäki T. Effect of chronic ethanol ingestion and gender on heart left ventricular p53 gene expression. Alcoholism: Clinical and Experimental Research. 2005 Aug;29(8):1368-73. [DOI] [PMID] [Google Scholar]
15. Goh JM, Bensley JG, Kenna K, Sozo F, Bocking AD, Brien J, Walker D, Harding R, Black MJ. Alcohol exposure during late gestation adversely affects myocardial development with implications for postnatal cardiac function. American Journal of Physiology-Heart and Circulatory Physiology. 2011; 300(2):H645-51. [view at publisher] [DOI] [PMID] [Google Scholar]
16. Guan Z, Lui CY, Morkin E, Bahl JJ. Oxidative stress and apoptosis in cardiomyocyte induced by high-dose alcohol. Journal of cardiovascular pharmacology. 2004 Dec 1;44(6):696-702. [view at publisher] [DOI] [PMID] [Google Scholar]
17. Morimoto T, Sunagawa Y, Kawamura T, Takaya T, Wada H, Nagasawa A, Komeda M, Fujita M, Shimatsu A, Kita T, Hasegawa K. The dietary compound curcumin inhibits p300 histone acetyltransferase activity and prevents heart failure in rats. The Journal of clinical investigation. 2008; 118(3):868-78. [view at publisher] [DOI] [PMID] [PMCID] [Google Scholar]
18. Yu L, Fan Y, Ye G, Li J, Feng X, Lin K, Dong M, Wang Z. Curcumin inhibits apoptosis and brain edema induced by hypoxia-hypercapnia brain damage in rat models. The American journal of the medical sciences. 2015; 349(6):521-5. [DOI] [PMID] [Google Scholar]
19. Rivera‐Espinoza Y, Muriel P. Pharmacological actions of curcumin in liver diseases or damage. Liver International. 2009 Nov;29(10):1457-66. [DOI] [PMID] [Google Scholar]
20. Maynard ME, Leasure JL. Exercise enhances hippocampal recovery following binge ethanol exposure. PloS one. 2013 Sep 30;8(9):e76644. [DOI] [PMID] [PMCID] [Google Scholar]
21. Hintz KK, Relling DP, Saari JT, Borgerding AJ, Duan J, Ren BH, Kato K, Epstein PN, Ren J. Cardiac overexpression of alcohol dehydrogenase exacerbates cardiac contractile dysfunction, lipid peroxidation, and protein damage after chronic ethanol ingestion. Alcoholism: Clinical and Experimental Research. 2003; 27(7):1090-8. [DOI] [PMID] [Google Scholar]
22. Kazemnejad A, Faramand S, Zayeri F, Salehi M, Yazdani Cherati J. Preparing the geographical maps of the relative death rate out of vasco-cardiac diseases in cities of the mazandaran province in 2008. Journal of Mazandaran University of Medical Sciences. 2012; 22(94):63-9. [Google Scholar]
23. Marnett LJ. Lipid peroxidation-DNA damage by malondialdehyde. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1999. 424(1): p. 83-95. [DOI] [Google Scholar]
24. Regan TJ. Alcohol and the cardiovascular system. Jama. 1990 Jul 18;264(3):377-81. [DOI] [PMID] [Google Scholar]
25. Cederbaum, A.I., Microsomal generation of reactive oxygen species and their possible role in alcohol hepatotoxicity. Alcohol and alcoholism (Oxford, Oxfordshire). Supplement, 1991. 1: p. 291-296. [Google Scholar]
26. Santana ET, Serra AJ, Silva Junior JA, Bocalini DS, Barauna VG, Krieger JE, Tucci PJ. Aerobic exercise training induces an anti-apoptotic milieu in myocardial tissue. Motriz: Revista de Educação Física. 2014; 20:233-8. [view at publisher] [DOI] [Google Scholar]
27. Osaki M, Oshimura MA, Ito H. PI3K-Akt pathway: its functions and alterations in human cancer. Apoptosis. 2004 Nov;9(6):667-76. [DOI] [PMID] [Google Scholar]
28. Siu PM, Bryner RW, Martyn JK, Alway SE. Apoptotic adaptations from exercise training in skeletal and cardiac muscles. The FASEB journal. 2004 Jul;18(10):1150-2. [DOI] [PMID] [Google Scholar]
29. Zhao YC. Effects of exercise training on myocardial mitochondrial miR-499-CaN-Drp-1 apoptotic pathway in mice. Zhongguo Ying Yong Sheng li xue za zhi= Zhongguo Yingyong Shenglixue Zazhi= Chinese Journal of Applied Physiology. 2015 May 1;31(3):259-63. [Google Scholar]
30. Jafari A, Pourrazi H, Nikookheslat S, Baradaran B. Effect of exercise training on Bcl-2 and bax gene expression in the rat heart. Gene, Cell and Tissue. 2015 Oct 31;2(4). [DOI] [Google Scholar]
31. Ziaaldini MM, Koltai E, Csende Z, Goto S, Boldogh I, Taylor AW, Radak Z. Exercise training increases anabolic and attenuates catabolic and apoptotic processes in aged skeletal muscle of male rats. Experimental gerontology. 2015; 67:9-14. [DOI] [PMID] [Google Scholar]
32. Su SH., Jen CJ, Chen HI. NO signaling in exercise training-induced anti-apoptotic effects in human neutrophils. Biochemical and Biophysical Research Communications, 2011; 405(1): p. 58-63. [DOI] [PMID] [Google Scholar]
33. Cannon CP, Branwald E, McCabe C. Pravastatin or atorvastatin evaluation and infection therapy (TIMI22). N Engl J Med, 2004; 350(15): p. 495-504. [Google Scholar]
34. Shin HJ, Lee JY, Son E, Lee DH, Kim HJ, Kang SS, Cho GJ, Choi WS, Roh GS. Curcumin attenuates the kainic acid-induced hippocampal cell death in the mice. Neuroscience letters. 2007; 416(1):49-54. [DOI] [PMID] [Google Scholar]
35. Bulku E, J Stohs S, Cicero L, Brooks T, Halley H, D Ray S. Curcumin exposure modulates multiple pro-apoptotic and anti-apoptotic signaling pathways to antagonize acetaminophen-induced toxicity. Current neurovascular research. 2012 Feb 1;9(1):58-71. [view at publisher] [DOI] [PMID] [Google Scholar]
36. Tao P, Yin H, and Ma Y. Study of the mechanisms of curcumin on mitochondrial permeability transition of hepatocytes in rats with sepsis. Zhonghua wei zhong bing ji jiu yi xue, 2014; 26(9): p. 666-670. [Google Scholar]
37. Samuhasaneeto S, Thong-Ngam D, Kulaputana O, Suyasunanont D, Klaikeaw N. Curcumin decreased oxidative stress, inhibited NF-B activation, and improved liver pathology in ethanol-induced liver injury in rats. Journal of biomedicine and biotechnology. 2009;2009. [DOI] [PMID] [PMCID] [Google Scholar]
38. Somasundaram S, Edmund NA, Moore DT, Small GW, Shi YY, Orlowski RZ. Dietary curcumin inhibits chemotherapy-induced apoptosis in models of human breast cancer. Cancer research. 2002 Jul 1;62(13):3868-75. [view at publisher] [Google Scholar]
39. Manna P, Das J, Ghosh J, Sil PC. Contribution of type 1 diabetes to rat liver dysfunction and cellular damage via activation of NOS, PARP, IκBα/NF-κB, MAPKs, and mitochondria-dependent pathways: Prophylactic role of arjunolic acid. Free Radical Biology and Medicine. 2010 Jun 1;48(11):1465-84. [DOI] [PMID] [Google Scholar]
40. Hu S, Xu Y, Meng L, Huang L, Sun H. Curcumin inhibits proliferation and promotes apoptosis of breast cancer cells. Experimental and therapeutic medicine. 2018 Aug 1;16(2):1266-72. [DOI] [PMID] [PMCID] [Google Scholar]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Jorjani Biomedicine Journal

Designed & Developed by : Yektaweb