Volume 11, Issue 2 (10-2023)                   Jorjani Biomed J 2023, 11(2): 19-23 | Back to browse issues page

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Naderi Noreini S, Ahmadi Nia Tabesh R, Malmir M, Ghafarizade A A. Protective effect of L-carnitine on sperm motility and membrane integrity in men asthenoteratozoospermia: An in vitro study. Jorjani Biomed J 2023; 11 (2) :19-23
URL: http://goums.ac.ir/jorjanijournal/article-1-889-en.html
1- Rastak Infertility Center, Arak, Iran
2- Department of Midwifery, Tuyserkan Branch, Islamic Azad University, Tuyserkan, Iran
3- Academic Center for Education, Culture and Research, Arak, Iran , Ghafarizade@acecr.ac.ir
Abstract:   (469 Views)
Background: Sperm preparation is a time-consuming process that makes a contribution to oxidative stress induction in assisted reproductive techniques.
Objective: This study aimed to evaluate the effect of L-carnitine on sperm parameters during semen preparation at different time intervals.
Materials and Methods: In this in vitro study, the semen samples of 50 asthenoteratozoospermic men were divided into the control and the L-carnitine groups (incubated with 0.5 mg/mL L-carnitine) (n=25/each). Total motility, progressive motility, sperm membrane integrity (by Hypo-osmotic swelling test), and lipid peroxidation (by Malondialdehyde level) were assessed in each group after 2, 4, and 6 hours. Afterward, samples were analyzed by the World Health Organization (2010) criteria.
Result: The results showed that in the control group total and progressive motility, and membrane integrity of the sperm were significantly decreased after 2 hr (P= 0.028, p= 0.019, and p= 0.025, respectively), while Malondialdehyde levels increased significantly after 4 hr (P= 0.018). Sperm parameters in the L-carnitine group increased significantly after 2 hr (P= 0.002), while Malondialdehyde levels decreased significantly compared to the control group in 6 hr (P < 0.001 vs. P =0.045).
Conclusion: Based on these results, L-carnitine can manage to conserve motility and sperm membrane integrity of sperm from the hazardous impact of oxidative stress and lipid peroxidation during sperm preparation.
Full-Text [PDF 625 kb]   (186 Downloads)    
Type of Article: Original article | Subject: Health
Received: 2022/02/5 | Accepted: 2023/09/30 | Published: 2023/12/30

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