Volume 9, Issue 1 (3-2021)                   Jorjani Biomed J 2021, 9(1): 44-54 | Back to browse issues page

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Sohrabi G, Malmir M, Ghafarizade A A, Faraji T. Ameliorative Effect of Melatonin Versus the Passage of Time and Lipid Peroxidation on Sperm Motility in Asthenotratospermic Men. Jorjani Biomed J. 2021; 9 (1) :44-54
URL: http://goums.ac.ir/jorjanijournal/article-1-791-en.html
1- Tabriz University of Medical Sciences International Branch Aras
2- Department of Midwifery, Tuyserkan Branch, Islamic Azad University, Tuyserkan, Iran
3- Academic Center for Education, Culture and Research, Arak, Iran , Ghafarizade@acecr.ac.ir
4- Academic Center for Education, Culture and Research, Arak, Iran
Abstract:   (787 Views)
Background and Objective: Downscale outcome in assisted reproductive techniques (ART) is associated with lipid peroxidation. Melatonin may have beneficial effects against lipid peroxidation and oxidative stress. This work was aimed to evaluate the ameliorative capacity of melatonin versus the passage of time and lipid peroxidation on sperm parameters from the men with ATS in the ART process.
Material and Methods: Semen samples were collected from 50 asthenoteratozoospermic (ATS) men. Samples were divided into control and melatonin group. The test group were incubated with 6 mM melatonin at 2, 4, 6, and 24 hours. Then total and progressive motility, membrane integrity, and Malondialdehyde levels (MDA) were evaluated. Statistical analysis was carried out by SPSS software (repeated-measures ANOVA).
Results: In the control group, total (p<0.01) and progressive (P<0.05) motility and also, membrane integrity (P<0.03) significantly decreased although, MDA levels of sperm significantly increased (*P<0.04). Moreover, in the melatonin group, the mentioned parameters significantly compensated compared to the control group (P<0.05).
Conclusion: In the outcome, melatonin in vitro treatment paves the way for motility and membrane integrity up-regulating in the ATS men.
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Type of Article: Original article | Subject: Basic Medical Sciences
Received: 2021/01/26 | Accepted: 2021/03/2 | Published: 2021/03/30

References
1. In Lucas, A. O., A, R., Bloom, B. R., Bok, S., Caldwell. et al. World Health Organization. In: J. C., & Chen, L. C. Part 1: Semen analysis, Standard procedures, WHO laboratory manual for the examination and processing of human semen. 5st ed. Geneva, Switzerland press; 2010. pp. 21-36.
2. Ghafarizadeh AA, Vaezi G, Shariatzadeh MA, Malekirad AA. Effect of in vitro selenium supplementation on sperm quality in asthenoteratozoospermic men. Andrologia. 2018; 50(2): e12869. [view at publisher] [DOI] [Google Scholar]
3. Jeulin C, Serres C, Jouannet P. The effects of centrifugation, various synthetic media and temperature on the motility and vitality of human spermatozoa. Reproduction Nutrition Développement. 1982;22: 81-91. [DOI] [Google Scholar]
4. Nabi A, Khalili MA, Halvaei I, Roodbari F. Prolonged incubation of processed human spermatozoa will increase DNA fragmentation. Andrologia.2014;46: 374-379. [view at publisher] [DOI] [Google Scholar]
5. Makker K, Agarwal A, Sharma R. Oxidative stress & male infertility. Indian Journal of Medical Research. 2009;129: 357-361. [Google Scholar]
6. Fanaei H, Khayat S, Halvaei I, Ramezani V, Azizi Y, Kasaeian A, Mardaneh J, Parvizi MR, Akrami M. Effects of ascorbic acid on sperm motility, viability, acrosome reaction and DNA integrity in teratozoospermic samples. Iranian journal of reproductive medicine. 2014;12: 103-109. [view at publisher] [Google Scholar]
7. Lenzi A, Picardo M, Gandini L, Dondero F. Lipids of the sperm plasma membrane: from polyunsaturated fatty acids considered as markers of sperm function to possible scavenger therapy. Human reproduction update. 1996;2: 246-56. [view at publisher] [DOI] [Google Scholar]
8. Bonanno O, Romeo G, Asero P, Pezzino FM, Castiglione R, Burrello N, Sidoti G, Frajese GV, Vicari E, D'Agata R. Sperm of patients with severe asthenozoospermia show biochemical, molecular and genomic alterations. Reproduction. 2016;152: 695-704. [DOI] [Google Scholar]
9. Pereira N, Naufel MF, Ribeiro EB, Tufik S, Hachul H. Influence of dietary sources of melatonin on sleep quality: a review. Journal of Food Science. 2020;85: 5-13. [view at publisher] [DOI] [Google Scholar]
10. Hassan MH, El Taieb MA, Fares NN, Fayed HM, Toghan R, Ibrahim HM. Men with idiopathic oligoasthenoteratozoospermia exhibit lower serum and seminal plasma melatonin levels: Comparative effect of night light exposure with fertile males. Experimental and Therapeutic Medicine. 2020;20: 235-42. [view at publisher] [DOI] [Google Scholar]
11. Fang Y, Zhang J, Li Y, Guo X, Li J, Zhong R, Zhang X. Melatonin-induced demethylation of antioxidant genes increases antioxidant capacity through RORα in cumulus cells of prepubertal lambs. Free Radical Biology and Medicine. 2019;131: 173-83. [view at publisher] [DOI] [Google Scholar]
12. Rodriguez C, Mayo JC, Sainz RM, Antolín I, Herrera F, Martín V, Reiter RJ. Regulation of antioxidant enzymes: a significant role for melatonin. Journal of pineal research. 2004;36: 1-9. [view at publisher] [DOI] [Google Scholar]
13. Özgür ME, Ulu A, Noma SA, Özcan İ, Balcıoğlu S, Ateş B, Köytepe S. Melatonin protects sperm cells of Capoeta trutta from toxicity of titanium dioxide nanoparticles. Environmental Science and Pollution Research. 2020;58: 1-1. [view at publisher] [DOI] [Google Scholar]
14. Akarca-Dizakar SÖ, Erdoğan D, Peker T, Coşkun Akçay N, Türkoğlu İ, Eşmekaya MA, Ömeroğlu S. Effects of co-administered melatonin, fructose and bisphenol A (BPA) on rat epididymis and sperm characteristics. Biotechnic & Histochemistry. 2020;95: 18-26. [DOI] [Google Scholar]
15. El-Raey M, Badr MR, Rawash ZM, Darwish GM. Evidences for the role of melatonin as a protective additive during buffalo semen freezing. American journal of animal and veterinary sciences. 2014;9: 252-62. [view at publisher] [DOI] [Google Scholar]
16. Lipovac MG. Antioxidative effect of melatonin on human spermatozoa. Archives of andrology. 2000;1: 44. 23-27. [view at publisher] [DOI] [Google Scholar]
17. Perumal P, Chang S, Sangma CT, Savino N, Khate K. Effect of melatonin on mobility and velocity parameters of mithun (Bos frontalis) semen preserved in liquid state (5° C). Journal of Experimental Biology and Agricultural Sciences. 2016;4: 52-60. [view at publisher] [DOI] [Google Scholar]
18. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Analytical biochemistry. 1996;239: 70-6. [view at publisher] [DOI] [Google Scholar]
19. Check ML, Kiefer D, Check JH, Hourani W, Long R. Treatment of sperm with subnormal host scores with chymotrypsin/viable pregnancy after IUI. Archives of andrology. 2002;48: 155-158. [view at publisher] [DOI] [Google Scholar]
20. Buege JA, Aust SD. [30] Microsomal lipid peroxidation. InMethods in enzymology. 1978; 52: 302-310. [DOI] [Google Scholar]
21. Mayorga-Torres BJ, Cardona-Maya W, Cadavid A, Camargo M. Evaluation of sperm functional parameters in normozoospermic infertile individuals. Actas Urológicas Españolas (English Edition). 2013;37: 221-227. [view at publisher] [DOI] [Google Scholar]
22. Verma A, Kanwar KC. Effect of vitamin E on human sperm motility and lipid peroxidation in vitro. Asian J Androl. 1999;1: 151-154. [view at publisher] [Google Scholar]
23. Kobayashi H, GIL‐GUZMAN EN, Mahran AM, Sharma RK, Nelson DR, AGARWAL A. Quality control of reactive oxygen species measurement by luminol‐dependent chemiluminescence assay. Journal of andrology. 2001;22: 568-574. [view at publisher] [Google Scholar]
24. Zakerabasali A, Keshavarz M, Mozafar A, Takhshid MA, Meshkibaf MH. Protective effects of vitamin E and selenium on spermatogenesis in adult male rat insulin-resistant. Journal of Fasa University of Medical Sciences. 2013;2: 308-13 [view at publisher] [Google Scholar]
25. Mahfouz RZ, du Plessis SS, Aziz N, Sharma R, Sabanegh E, Agarwal A. Sperm viability, apoptosis, and intracellular reactive oxygen species levels in human spermatozoa before and after induction of oxidative stress. Fertility and sterility. 2010;93: 814-21. [view at publisher] [DOI] [Google Scholar]
26. Sun TC, Li HY, Li XY, Yu K, Deng SL, Tian L. Protective effects of melatonin on male fertility preservation and reproductive system. Cryobiology. 2020;95: 1-8. [view at publisher] [DOI] [Google Scholar]
27. Fernando S, Rombauts L. Melatonin: shedding light on infertility? a review of the recent literature. Journal of ovarian research. 2014;7: 98-104. [view at publisher] [DOI] [Google Scholar]
28. Espino J, Ortiz Á, Bejarano I, Lozano GM, Monllor F, García JF, Rodríguez AB, Pariente JA. Melatonin protects human spermatozoa from apoptosis via melatonin receptor-and extracellular signal-regulated kinase-mediated pathways. Fertility and sterility. 2011;95: 2290-2296. [view at publisher] [DOI] [Google Scholar]
29. Ghafarizadeh AA, Malmir M, Naderi Noreini S, Faraji T, Ebrahimi Z. The effect of vitamin E on sperm motility and viability in asthenoteratozoospermic men: In vitro study. Andrologia. 2020;24: e13891. [view at publisher] [DOI] [Google Scholar]
30. Malmir M, Naderi Noreini S, Ghafarizadeh A, Faraji T, Asali Z. Ameliorative effect of melatonin on apoptosis, DNA fragmentation, membrane integrity and lipid peroxidation of spermatozoa in the idiopathic asthenoteratospermic men: In vitro. Andrologia. 2020;52: e13944. [view at publisher] [DOI] [Google Scholar]
31. Naderi Noreini S, Malmir M, Ghafarizadeh A, Faraji T, Bayat R. Protective effect of L‐carnitine on apoptosis, DNA fragmentation, membrane integrity and Lipid peroxidation of spermatozoa in the asthenoteratospermic men. Andrologia. 2020;25: e13932. [view at publisher] [DOI] [Google Scholar]
32. Ghafarizadeh A, Malmir M, Naderi Noreini S, Faraji T. Antioxidant effects of N‐acetylcysteine on the male reproductive system: A systematic review. Andrologia. 2021;53: e13898. [view at publisher] [DOI] [Google Scholar]
33. Malmir M, Faraji T, Ghafarizadeh AA, Khodabandelo H. Effect of nonylphenol on spermatogenesis: A systematic review. Andrologia. 2020;52: e13748. [view at publisher] [DOI] [Google Scholar]

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