2024-03-28T19:22:54+03:30 http://mlj.goums.ac.ir/browse.php?mag_id=23&slc_lang=en&sid=1
23-665 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 2015 4 01 0 0 http://mlj.goums.ac.ir/article-1-665-en.pdf
23-598 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 Drug-Resistant HIV-1 RT Gene Mutations in Patients under Treatment with Antiretroviral Drugs (HAART) in Iran Gol Mohammadi, R Tabaraei, A Abbasi, A Khademi, N Mahdavian, B Javid, N Kaleji, H kamasi,A Bazoori, M Moradi, A abmoradi@yahoo.com Abstract Background and Objective: Highly Active Antiretroviral Therapy (HAART) can effectively prevent the progression of HIV-1 replication and increase life expectancy. There are numerous causes of treatment failure and the leading one is drug resistance. Thus, we aimed to determine the HIV RT gene drug resistance mutations in patients treated with antiretroviral medications. Material and Methods: In this cross - sectional study, venous blood was taken from 130 HIV-positive patients treated with antiretroviral medications. In order to determine drug resistance mutations, RT-PCR and PCR steps were performed using RT gene specific primers. Subtypes and mutations in the virus genome were determined using the Stanford HIV drug resistance sequence database. Results: In 122 treating patients, most of the major mutations were associated with nucleoside and non-nucleoside drugs. subtype A in 66.4%, subtype D in 26.2% and subtype B in 7.4% of the participants were reported. They were resistant to Nucleoside RT Inhibitor drugs (23.7%) and Non-Nucleoside RT Inhibitor drugs(30.3%). The highest were related to Nevirapine (21.3%) and Efavirenz (19.7%) and the lowest to both Tenofovir and Zidovudine (91.5%). Conclusion: The use of two nucleoside RT inhibitor drugs combined with one protease inhibitor drug could be effective in the treatment of HAART. Key words: HIV, Nucleoside RT Inhibitor, Non- Nucleoside RT Inhibitor HIV Nucleoside RT Inhibitor Non- Nucleoside RT Inhibitor 2015 4 01 1 8 http://mlj.goums.ac.ir/article-1-598-en.pdf
23-599 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 Immunologic Evaluation of DNA Vaccine Encoding Influenza Virus M2 Gene in Type A- Influenza Mice Model Shaffifar, M. Tabarraei, A. Sajadian, A. Fotouhi, F Ghaemi, A ghaem_amir@yahoo.com Abstract Background and Objective: The M2 gene expressing the conserved protein in influenza virus can be used to make a single-dose vaccine with permanent immunity. Material and Methods: The mice were allocated to one case group immunized with pcDNA3-M2 and two control groups with pcDNA and PBS, in three dozes with interval of two weeks. Two weeks after the last injection, Cellular immunity was analyzed by MTT lymphocyte proliferation, interferon gamma (IFN-gamma) and interleukin 4 (IL-4) ratio assays. The remaining animals were challenged with PR8 virus. Results: The production rate of IFN8 and IL4 in pcDNA - M2 group was higher than that of control groups (P >0.0001). Given the results of lymphocyte proliferation, Stimulation index (SI) in vaccinated mice was significantly higher than that of control groups (P<0.05). In comparison with mortality rate of 100% in control groups , the animals Challenged with PR8 vaccine had a 50% fatal rate implying a high protection level for this vaccine. Conclusion: The pcDNA3-M2 Vaccine can be considered as a promising vaccine against influenza infections. Keywords: Influenza Virus, Gene Vaccine, M2 Protein 2015 4 01 9 16 http://mlj.goums.ac.ir/article-1-599-en.pdf
23-600 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 Molecular Detection of Salmonella Serovar Isolated from Eggs Monadi, M Kargar, M mkargar@jia.ac.ir Naghiha, A Najafi, A Mohammadi, R Abstract Background and Objective: Salmonellosis is the most common type of food poisoning in developed and developing countries that is caused by Salmonella serotype. Hence, we aimed to identify the Salmonella serovars in eggs obtained from Kohgiluyeh and Boyerahmad province and to evaluate antibiotic resistance of the isolated strains. Material and Methods: In this study, 210 eggs were collected from Kohgiluyeh and Boyerahmad Province. The bacteria were isolated and identified using biochemical tests. After extraction of genomic DNA, Salmonella gender, Salmonella enteritidis and Salmonella typhimurium were investigated by invA, fliC and sefA primers, respectively, using Multiplex PCR method. Results: Of 210, 14 (6.66%) were contaminated with Salmonella. Of these, 12 (5.71%) were Salmonella typhimurium and 2 (0.95%) were related to Salmonella spp. None of the samples were contaminated with Salmonella enteritidis. The highest resistance was related to penicillin (100%) and neomycin (78.57%). Conclusion: Salmonella typhimurium is the predominant serovar causing contamination in the eggs of this Province. Given the wide spread of antibiotic resistance in different serotypes of Salmonella, we recommend avoiding of indiscriminate use of antibiotics in livestock and poultry. Keywords: Salmonella, Drug Resistance, Antibiotic, Multiplex PCR, Kohgiluyeh and Boyerahmad Salmonella Drug Resistance Antibiotic Multiplex PCR Kohgiluyeh and Boyerahmad 2015 4 01 17 24 http://mlj.goums.ac.ir/article-1-600-en.pdf
23-601 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 Distribution of Anti-leptospira Antibodies in the Sera of Patients Suspected Leptospirosis Roohi, Z Moradi Bidhendi, S Khaki, P s.bidhendi@rvsri.ac.ir Abstract Background and Objective: Leptospirosis is a zoonosis infectious disease that is prevalent in tropical and subtropical regions and is caused by the pathogenic serovars of leptospires. Hence, we aimed at investigating the prevalence of antibodies against these bacteria in the blood samples of suspected leptospirosis. Material and Methods: the human serum samples (N = 130) were obtained from patients clinically suspected leptospirosis. The Serum level of IgM antibodies were studied by ELISA kit (PrioCHECK) in Razi Vaccine and Serum Research Institute (Karaj), 2011-2012. Results: Anti-leptospira IgM class was observed in 21(16%) samples. The relative distribution of the disease was reported in men (80.95%), women (19.04%), and farmers (30.95%) and in 20-40-year group (57.14%). Contact with contaminated water was the most common cause of infection (52.38%) and fever was the most common sign of Leptospirosis (72.2%). Conclusion: Due to the occurrence of anti-leptospira antibodies in 16% of suspected cases, it is recommended that routine ELISA be done at least in major diagnostic centers. Keywords: Leptospira, Leptospirosis, Human, ELISA Leptospira Leptospirosis Human ELISA 2015 4 01 25 31 http://mlj.goums.ac.ir/article-1-601-en.pdf
23-602 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 Compression of Histopathology, Culture and Rapid Urease Test in Diagnosis of Helicobacter Pylori in Gastric Biopsy Specimens Seyedeyn Khorasani, M Yousefi Mashouf, R Majlesi, A Jaefari, M Alikhani, MY alikhani@umsha.ac.ir Abstract Background and Objective: Helicobacter pylori (H. Pylori) infection is related to chronic gastritis, peptic ulcer, duodenal ulcer and gastric cancer. Thus, identification and treatment of the infection have a considerable importance. The aim of this study was to compare three methods of Histopathology, Culture and Rapid Urease test (RUT) in identification of H. Pylori in gastric biopsy specimens. Material and Methods: The participants were 153 patients (64 women and 89 men) suffering from digestive complaints, who referred to the endoscopy department of Shahid Beheshi Hospital in Hamadan, Iran. Three gastric biopsy samples were collected from each patient and examined by standard RUT, Histopathology and culture methods for diagnosis of H. Pylori. Results: Out of 153 patients, 69.9%, 27.4% and 2.6 % had gastritis, gastric ulcer and gastric cancer, respectively. The rate of infection with Urease test, culture and histology were identified 49.7%, 54.2%, and 89.5%, respectively. The sensitivity and specificity of the RUT result at first hour and after the first up to 24 hours were 55.4% and 80%, and 55.4% and 66/7%, respectively. The sensitivity and specificity of culture method were 60.6% and 100%, respectively. Conclusion: Based on the results, Histopathology method has a more sensitivity than both Culture method and rapid urease test for diagnosis of H. Pylori, and RUT is more specific when done in the first hour rather than after the first hour. Key words: Helicobacter pylori, rapid urease test methods, RUT, Histopathology Helicobacter pylori rapid urease test methods RUT Histopathology 2015 4 01 32 38 http://mlj.goums.ac.ir/article-1-602-en.pdf
23-603 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 Bacterial Contamination and Antibiotic Resistance of Staphylococcus Aureus Isolated from Automated Teller Machine Moshtaghi, H Parsa, M minaparsa14@yahoo.com Abstract Background and Objective: Automated Teller Machine (ATMs) is likely to be contaminated with various microorganisms specially pathogen germs. This may be due to their exposure to dust and their vast dermal contact with multiple users. This study investigated the bacterial contamination on the keyboard of ATMs and drug resistance of the bacteria isolated from them. Material and Methods: the keyboards of 50 ATMs in Shahrekord city, Iran, were examined from October 2012 to February 2013. The sterile swab sticks moistened with Triptose soy broth were used for sampling. The bacteriological tests used were culture, biochemical test and agar disk diffusion method for antibiogram. Results: All the samples were found to be contaminated with Coagulase negative staphylococci (57.54%), Bacillus species (21.92%), Staphylococcus aureus (19.18%) and coliform bacteria (1.36%). The resistance of Staphylococcus aureus was 92.8% to penicillin, 85.7% to amoxicilin، 71.4% to ampicillin, 57.1% to nytrofuran, 50% to tetracycline, 42.8% to erythromycin, 42.8% to gentamycin, 14.2 % to ciprofloxacin, 7.1% to trimethoprim and sulfamtuksazul. All species were susceptible to, ofloxacine, chloramphenicol, clindamycin, tobramycin, vancomycin and cefotaxime. Conclusion: given the presence of pathogens on ATMs and their role in transferring the contamination, we recommend considering personal hygiene and periodically disinfecting the keyboards to reduce contamination. Keywords: ATMs, Bacterial Contamination, Antibiogram ATMs Bacterial Contamination Antibiogram 2015 4 01 39 44 http://mlj.goums.ac.ir/article-1-603-en.pdf
23-604 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 Designing and Construction of Recombinant Plasmid Consisting of Basic Fibroblast Growth Factor and Immunodominant Fragments of Pseudomonas Exotoxin H Haghighatfard dr.norozi@yahoo.com Y Yazdani, Y. Abstract Background and Objective: the inhibition of tumor-associated angiogenesis can significantly reduce the tumor proliferation. The basic fibroblast growth factor (bFGF), an important angiogenic factor, is considered as a potential therapeutic target for cancer therapy. The purpose of this study was evaluating, designing and construction of new recombinant DNA molecule in order to have efficient expression of a fusion protein consisting of the bFGF and immunodominant epitopes of Pseudomonas toxin. Material and Methods: Different types of peptide linker, codon adaptation index (CAI) and adding signal peptide were considered in designing of immunogenic coding sequence. After software evaluation, the recombinant DNA molecule was ordered in the puc57 cloning vector. Then, coding sequence inserted into the multiple cloning site of pET28-a plasmid. Finally, PCR and enzymatic digestion tests were done for evaluation of recombinant expression vector. Results: Optimization of DNA sequence, codon adaptation index (CAI) increased from 0.69 to 0.83 and GC content decreased from 61 to 54.77. The presence of 1214-bp PCR product and 1029-bp one obtaining from enzymatic digestion confirmed the correction of the cloning process. Conclusion: According to the previous studies, it is the first work for designing, optimizing and synthesis of recombinant DNA consisting of bFGF and immunodominant epitopes of Pseudomonas toxin. Keywords: Tumor angiogenesis, immunodominant epitopres of Pseudomonas toxin, Fibroblast growth factor 2, DNA 2 software Tumor angiogenesis immunodominant epitopres of Pseudomonas toxin Fibroblast growth factor 2 DNA 2 software 2015 4 01 45 52 http://mlj.goums.ac.ir/article-1-604-en.pdf
23-605 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 In Vitro Comparison of MIC Crude Extracts of Active Actinomycetes Isolated with Terbinafine, Griseofulvin Ketoconazole and Fluconazole against Microsporum Canis, Microsporum Gypseum and Trichophyton Mentagrophytes Keikha, N Nasserkeikha@yahoo.com Ayatollahi Mousavi, SA Nakhaei, AR Amanloo, S Shahidi, GH Amiri, S Abstract Background and Objective: Dermatophytes are the fungi that have the ability to attack the keratinized tissues such as the skin, hair and nails. Infections caused by these organisms are named dermatophytosis. We aimed to compare Minimum inhibitory concentration (MIC) of Crude extracts of Active Actinomycete Isolates with Terbinafine, Griseofulvin, Ketoconazole and Fluconazole Drugs against Microsporum Canis, Microsporum gypseum and Trichophyton mentagrophytes. Material and Methods: In this experimental study, in order to find MIC by actionmycete, 100 isolates were studied and then crude extracts of the active actinomycete isolates were prepared in sterile conditions. Finally, the crude extracts obtained at different concentrations were used to obtain the MIC of Microsporum Canis, Microsporum gypseum and Trichophyton mentagrophytes. Moreover, various concentrations of the drugs such as terbinafine, griseofulvin, ketoconazole and fluconazole in solvent Dimethyl sulfoxide (DMSO) were prepared and their growth inhibitory effect was evaluated and then compared with the results obtained from the crude extract of active actinomycete isolates. Results: the crude extracts obtained from active Actioiomycetes isolates and the drugs such as terbinafine, griseofulvin, ketoconazole and fluconazole, in a dose-dependent manner, could inhibit the growth of Microsporum Canis, Microsporum gypseum and Trichophyton Mentagrophytes. Conclusion: compared to MIC of Crude extract of active actinomycete isolates, Terbinafine has a significant effect on the growth inhibition in all of the fungal Dermatophytes and then griseofulvin, ketoconazole and fluconazole are in the next rank, respectively. Keywords: Actinomycetes Crude Extract, Microsporum Canis, Microsporum Gypseum, Terbinafine 2015 4 01 53 60 http://mlj.goums.ac.ir/article-1-605-en.pdf
23-664 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 2015 4 01 61 69 http://mlj.goums.ac.ir/article-1-664-en.pdf
23-666 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 2015 4 01 61 69 http://mlj.goums.ac.ir/article-1-666-en.pdf
23-668 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 Identification of electrophoretic proteins pattern of Fusaium verticillioides isolates from maize by SDS-PAGE. R Daie Ghazvini A Khosrav SA Ghiasian 2015 4 01 61 69 http://mlj.goums.ac.ir/article-1-668-en.pdf
23-606 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 Effect of α-lipoic Acid on Hemolytic Activity of Iranian Vipera Lebetina Venom Amoozgari, Z Zamoozgari277@yahoo.com Salehi pour bavarsad, S Noorbehbahani, M Abstract Background and Objective: Snake venom is a complex of several toxic elements and enzymes. It has the agents with the ability to destroy cellular and subcellular membrane and to bring about hemolysis of red blood cells (RBC). Two types of direct and indirect hemolytic activity are known in snake venom in that phospholipase A2 is responsible for the indirect lysis. The aim of this study was to investigate the effect of α-lipoic acid on hemolytic activity of Iranian Vipera Lebetina venom. Material and Methods: Protein concentration of the crude venom of Vipera Lebetina was determined using bovine serum albumin as a standard. Direct hemolytic activity of venom was determined by using the Human RBC and Indirect hemolytic activity was assayed on RBC in the presence of egg yolk. Then, α-lipoic acid with different concentrations in 100 mM Tris-HCL buffer was applied and its effect on hemolysis of RBC was studied. Results: direct hemolytic activity on RBC was not observed while its indirect activity was detected to be increased proportional to different concentration of α-lipoic acid. The range of indirect hemolysis was increased up to 60% by 60µm α-lipoic acid. Conclusion: Not only has α-lipoic acid no inhibitory effects on the hemolytic activity of Iranian Vipera Lebetina venom but also has the positive effects on it. Keywords: Iranian Vipera Lebetina Venom, Direct Hemolytic, Indirect Hemolytic, α-Lipoic Acid, Phospholipase A2 Iranian Vipera Lebetina Venom Direct Hemolytic Indirect Hemolytic α-Lipoic Acid Phospholipase A2 2015 4 01 70 75 http://mlj.goums.ac.ir/article-1-606-en.pdf
23-608 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 Effect of Regular Aerobic Training and Arbutin on Cardiac Total Oxidant and Antioxidant Status in Alloxan-Induced Diabetic Rat M shojaee Farzanegi, P M habibian Abstract Background and Objective: Diabetes mellitus is associated with cardiomyopathic changes, can be mediated by an oxidative stress. We aimed to study the effects of regular aerobic training and arbutin supplementation on total oxidant status (TOS) and total antioxidant (TAS) status in the cardiac tissue of diabetic rats. Material and Methods: fourty-two male Wistar rats with an average weight of 195 to 220 gr were randomly divided into 6 groups (7 rats per group) of control, diabetes, Arbutin, diabetes + Arbutin, diabetes + aerobic training and diabetes + aerobic training + Arbutin. Swimming training protocol consisted of 5 days/week for 6 weeks and each session was 5-36 min/day. Diabetes was induced with alloxan intraperitoneally and Arbutin (50 mg/kg) was administered subcutaneously. Results: Induced- diabetes significantly increased TOS and decreased TAS in rat heart tissue (P = 0.000). Six weeks of supplementation with Arbutin, aerobic training and combination of aerobic training and Arbutin supplementation were associated with a significant decrease in TOS (88%, 91% ,103% Respectively ) and increase in TAS (33% ,62% ,67% Respectively ). Conclusion: Compared to arbutin, aerobic training can be more effective in creating adaptation in the antioxidant defense system. Keywords: Aerobic Training, Arbutin, Total Oxidant Status, Total Antioxidant Status, Diabetes. Aerobic Training Arbutin Total Oxidant Status Total Antioxidant Status Diabetes. 2015 4 01 76 83 http://mlj.goums.ac.ir/article-1-608-en.pdf
23-610 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 Correlation between Serum T3 Resin Uptake and Serum Lipid Profile Dinarvand, GH Veis Raygani, A vaisira@yahoo.com Ghanbari, N Abstract Background and Objective: T3 resin uptake test indicates the availability of thyroxin binding globulin (TBG), which is the carrier of a large amount of T3 and T4 in blood. We aimed to study the relationship between serum T3 Resin Uptake and serum lipids profile. Material and Methods: The participants aged 14.80 ± 38.29 were both males (59 29.5%) and females (141 70.5 %). T3 Resin Uptake was measured by Immunoassay method and serum lipid profile components (cholesterol, triglycerides, HDL-C, LDL-C, and VLDL) were measured by the kit. Results: Significant negative correlation was observed between serum T3 Resin Uptake and cholesterol, triglycerides and LDL-C (P≤ 0.05), but that was not the case for HDL-C and VLDL. Conclusion: Serum T3 Resin Uptake can be evaluated to assess the risk factors related to cardiovascular disorders. Keywords: Cholesterol, Triglycerides, T3 Resin Uptake, lipoprotein, Lipid Profile Cholesterol Triglycerides T3 Resin Uptake lipoprotein Lipid Profile 2015 4 01 84 89 http://mlj.goums.ac.ir/article-1-610-en.pdf
23-611 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 Evaluation of Serum CRP and Vitamin D in Rheumatoid Arteritis Patients and Healthy People Abdollahi, E. (BSc) Tavasolian, F. (BSc) Vakili, M. (PhD) Samadi, M. (PhD) samadi@ssu.ac.ir Abstract Background and Objective: C- reactive protein (CRP), as an acute phase reactant and a reliable marker of inflammation, increases due to inflammatory diseases such as Rheumatoid Arteritis and infectious conditions. New evidence shows that Vitamin D may have important effects on adjusting and reducing the Immune Responses. The aim of this study was to evaluate the association between serum vitamin D as an immunomodulator factor and CRP as an inflammatory factor in Arteritis Patients. . Material and Methods: The CRP and Vitamin D were evaluated in Rheumatoid Arteritis patients confirmed by Medical records (40 men and 40 women) and in 80 healthy adult people with normal CRP and Vitamin D and no history of arteritis (40 men and 40 women). Torbidometry was used to measure CRP and Eliza for Vitamin D. Results: In patient group, the mean of CRP and Vitamin D were 95.9±9.1 Mlg/lit and 9.17±2.9 Mlg/lit, resoectively. There was a significant inverse correlation between C-reactive protein and vitamin D in Rheumatoid Arteritis patients (Pvalue= 0.03 Pearson correlation: -0.62) and that was the case for healthy people (p value: 0.04 Pearson correlation: -0.73). Conclusion: Based on the findings, inverse correlation is observed between serum vitamin D and CRP level. Keywords: Vitamin D, CRP, Rheumatoid Arteritis Vitamin D CRP Rheumatoid Arteritis 2015 4 01 90 96 http://mlj.goums.ac.ir/article-1-611-en.pdf
23-612 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 A Simplified Van Erth Single Nucleotide Polymorphism (SNP) Typing Method of Bacillus Anthracis Applicable by Traditional Thermocycler Machines Najafi Olya, Z. (BSc) Tadayon, K. (PhD) k.tadayon@rvsri.ac.ir Ghaderi, R. (BSc) Abstract SNP typing is now a well-established genotyping system in Bacillus anthracis studies. In the original standard method of Van Erth, SNPs at 13 loci of the B. anthracis genome were analyzed. In order to simplify and make appropriate this expensive method to low-budget laboratory settings, 13 primer pairs targeting the 13 corresponding SNPs were designed. Besides, a universal PCR protocol was developed to enable simultaneous amplification of all loci by conventional PCR machines. The efficiency of this approach was approved by applying on nine isolates of B. anthracis. We recommend using this modified procedure as an efficient alternative to Van Erth method until developing newer and affordable techniques. Keywords: Bacillus Anthracis, Genotyping, SNPs, PCR Bacillus Anthracis Genotyping SNPs PCR 2015 4 01 97 103 http://mlj.goums.ac.ir/article-1-612-en.pdf
23-613 2024-03-28 10.1002
Medical Laboratory Journal mljgoums 2538-4449 10.52547/mlj 2015 9 1 Relationship between Personality Characteristics and Organizational Commitments of Clinical Laboratory Personnels Ameri, E. (BSc) Saeedi, P. (PhD) dr.parvizsaeedi@yahoo.com AA Ayatollahi Abstract Background and Objective: Efficient human resource is the major advantage of an organization over others. Commitment to organization not only may reduce sick leave, delays and displacements but also give rise to a significant increase in productivity, more vitality among staff, better attainment of the organizations' goals and personal aims. This study aimed to evaluate the relationship between personal characteristics and organizational commitment among medical laboratory staff. Material and Methods: This correlational study was conducted on 105 medical laboratory staff selected randomly in Gorgan, 2010-2011. The instruments for data collection were NEO personality inventory and Meyer and Allen's model of organizational commitment. Results: The relationship between organizational commitment and the variables such as neuroticism, openness, agreeableness and Conscientiousness was positively significant but that was not the case for extroversion characteristic. Conclusion: Given the positive relationship between personality characteristics and organizational commitment, we recommend improving these characteristics to enhance staff commitment to organization. Keywords: Organizational Commitment, Personality Characteristics, Laboratory Staff Organizational Commitment Personality Characteristics Laboratory Staff 2015 4 01 104 109 http://mlj.goums.ac.ir/article-1-613-en.pdf