@article{ author = {Azadi, Fahimeh and Rezanezhadi, Masoomeh and Bagheri, Hanieh and Alhusseini, Laith B and Joshaghani, Hamid Rez}, title = {Identification of Mycobacterium tuberculosis and Rifampin Resistance in Pulmonary and Extra-pulmonary Clinical Specimens Using the Gene Xpert MTB/RIF Assay}, abstract ={Background and objectives: Tuberculosis (TB) is a serious public health problem and a significant diagnostic and therapeutic challenge worldwide. Molecular diagnostic techniques are crucial parts of the World Health Organization’s new tuberculosis control strategy. This study aims to identify Mycobacterium tuberculosis and rifampin resistance in pulmonary and extra-pulmonary clinical specimens using the Gene Xpert MTB/RIF assay. Methods: The study was carried out on 220 specimens from pulmonary and extra-pulmonary TB patients that were sent to the Kavosh Laboratory in Gorgan (Iran) during 2018-20. The Gene Xpert MTB / RIF method was applied to detect M. tuberculosis and rifampin resistance. Results: Of 220 specimens, 15 (6.81%) were found to be positive, four (26.6%) of which were related to pulmonary and 11(73.3%) to extra-pulmonary specimens. None of the positive samples was resitant to rifampin according to assay. Conclusion: Our findings demonstrate that the Gene Xpert MTB/RIF is able to accurately detect M. tuberculosis in pulmonary and extra-pulmonary specimens. The accurate and early diagnosis of TB infection allows timely therapeutic intervention, which is beneficial not only for the patient but also for possible contacts.}, Keywords = {Mycobacterium tuberculosis,Tuberculosis Multidrug-Resistant,Rifampin,}, volume = {15}, Number = {4}, pages = {1-5}, publisher = {school of paramedicine, golestan university of medical sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.52547/mlj.15.4.1}, url = {http://mlj.goums.ac.ir/article-1-1314-en.html}, eprint = {http://mlj.goums.ac.ir/article-1-1314-en.pdf}, journal = {Medical Laboratory Journal}, issn = {}, eissn = {2538-4449}, year = {2021} } @article{ author = {zakeri, Mana and taheri, Forough and rezanezhadi, masoomeh and zaimy, Mohammad ali and Abdullah, alaa laebi}, title = {A Case Report of a Man with Klinefelter Syndrome Having a Healthy Neonate with Normal Karyotype}, abstract ={Background: Klinefelter syndrome (KS) also known as 47, XXY is one of the most prevalent chromosomal abnormalities among men. Infertility is one of the most primary features of this condition. However, there are some other associated features such as thin and tall appearance, absent, delayed or incomplete puberty, small and firm testicles, small penis and gynecomastia. Case description: We herein report a patient with mosaic KS whose karyotype consisted of 47, XXY/46, XY. The case’s wife had two miscarriages, followed by a healthy girl with a normal karyotype who was born taller than the average at the age of two. Conclusion: Mosaic KS dramatically increases the chance of having healthy offspring with normal genetic patterns without performing artificial insemination methods compared to those with complete KS.}, Keywords = {Klinefelter Syndrome,Chromosome Aberrations,Newborn,}, volume = {15}, Number = {4}, pages = {6-8}, publisher = {school of paramedicine, golestan university of medical sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.52547/mlj.15.4.6}, url = {http://mlj.goums.ac.ir/article-1-1325-en.html}, eprint = {http://mlj.goums.ac.ir/article-1-1325-en.pdf}, journal = {Medical Laboratory Journal}, issn = {}, eissn = {2538-4449}, year = {2021} } @article{ author = {Yazdani, Bahar and Anani, Hussein and baluchi, iman and KalantaryKhandany, Behjat and Hassanshahi, Gholamhossei}, title = {Effect of Chemotherapy on Serum Level of CCL2 in Acute Myeloid Leukemia Patients with Monocytic Differentiation}, abstract ={Background and objectives: Acute myeloid leukemia (AML) is a malignancy that involves the bone marrow and peripheral blood. Some chemokines play a role in the progression, migration and tumor initiation and are therefore associated with poor prognosis. CCL2 promotes tumor growth and is associated with poor prognosis in AML patients. We investigated effects of chemotherapy on serum level of CCL2 in AML patients. Methods: Throughout this case-control study, blood samples were collected from 25 healthy individuals and 25 AML (M4 and M5) patients before and after the first stage of the current chemotherapy regimen (7+3). Serum level of CCL2 was measured using commercial ELISA kits. Data were analyzed in SPSS 22 using the two-sample t-test and paired t-test. Results: Before chemotherapy, serum level of CCL2 was significantly higher in the patients than in the healthy controls. Following chemotherapy, the serum level of CCL2 reduced significantly to a level comparable to that of the healthy controls. Conclusion: The current chemotherapy (7+3) can effectively inhibit CCL2 in AML patients.}, Keywords = {Leukemia, Myeloid, Acute,Chemokine CCL2,Chemokines,}, volume = {15}, Number = {4}, pages = {9-14}, publisher = {school of paramedicine, golestan university of medical sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.52547/mlj.15.4.9}, url = {http://mlj.goums.ac.ir/article-1-1302-en.html}, eprint = {http://mlj.goums.ac.ir/article-1-1302-en.pdf}, journal = {Medical Laboratory Journal}, issn = {}, eissn = {2538-4449}, year = {2021} } @article{ author = {Hamta, Ahmad and Hosseinpour, Maliheh and Komijani, Maji}, title = {Association of Interleukin-16 Gene Polymorphisms rs11556218 T/G and rs4072111 C/T with Risk of Breast Cancer in an Iranian Population}, abstract ={Background and objectives: Breast cancer is the leading cause of death in women. Cytokines regulate the intensity and continuity of immune response by balancing cell-mediated immunity and humoral responses. This study aimed to investigate the relationship between two polymorphisms of the interleukin-16 (IL-16) gene and risk of breast cancer. Methods: Blood samples were collected from 80 breast cancer patients and 80 healthy individuals. Polymorphisms rs11556218 T/G and rs4072111 C/T were investigated by polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR). Then, genotype and allele frequency distributions were evaluated in each group using the SPSS software (version 24). Results: The frequency of genotype rs11556218 differed significantly between the patients and controls (P=0.007). The TG genotype (odds ratio [OR]= 2.471, 95% confidence intervals [CI]: 1.229-4.965, P= 0.001) and total TG+GG genotypes (OR= 3.095, 95% CI: 1.624-5.899, P= 0.001) had a significant relationship with increased risk for breast cancer. The allele and genotype frequencies of rs4072111 C/T polymorphism did not differ significantly between the patients and controls. Conclusion: Our findings suggest that the rs11556218 T/G polymorphism of the IL-16 gene may be associated with susceptibility to breast cancer.}, Keywords = {Breast Neoplasms,Interleukin-16,Single Nucleotide,}, volume = {15}, Number = {4}, pages = {15-20}, publisher = {school of paramedicine, golestan university of medical sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.52547/mlj.15.4.15}, url = {http://mlj.goums.ac.ir/article-1-1311-en.html}, eprint = {http://mlj.goums.ac.ir/article-1-1311-en.pdf}, journal = {Medical Laboratory Journal}, issn = {}, eissn = {2538-4449}, year = {2021} } @article{ author = {arbabian, toktam and Rezaeshirazi, reza and FarzanehHesari, Amin and Asgharpour, Habib}, title = {Does Physical Activity Level Affect Homocysteine and Obesity Variables in Women with Cardiovascular Disease?}, abstract ={Background and objectives: Physical activity has long been considered as the cornerstone of interventions for reducing the burden of cardiovascular diseases (CVDs). Homocysteine and obesity indices are important indicators of CVD risk. The present research aimed to evaluate homocysteine and obesity variables in female CVD patients and healthy counterparts with different physical activity levels. Methods: This study was carried out on 85 CVD patients (40-55 yr, n=41, 60-75 yr, n=44) and 80 healthy women (40-50 yr, n=40, 60-75 yr, n=40). Subjects were divided into three groups based on their physical activity level: sedentary, moderately active and active. Serum homocysteine, body roundness index (BRI), a body shape index (ABSI), body mass index (BMI) and waist circumference (WC) were measured. Data were analyzed using the independent t-test and one-way ANOVA. Results: Physically active middle-aged CVD patients had significantly lower BRI (p=0.022) and homocysteine (p=0.008) levels compared with the sedentary counterparts. In addition, physically-active old CVD patients had significantly lower BRI (p=0.041), ABSI (p=0.011) and homocysteine (p=0.001) compared with the sedentary counterparts. Physically-active healthy middle-aged individuals had significantly lower BRI (p=0.013) and BMI (p=0.008) levels compared with sedentary counterparts. Active elderly subjects also had significantly lower BRI (p=0.001) and WC (p=0.008) compared with the sedentary counterparts. Conclusion: Physical activity can reduce the risk of CVD and improve health status of women by reducing homocysteine, BRI and ABSI.}, Keywords = {Exercise,Cardiovascular Diseases,Homocysteine,Obesity,}, volume = {15}, Number = {4}, pages = {21-27}, publisher = {school of paramedicine, golestan university of medical sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.52547/mlj.15.4.21}, url = {http://mlj.goums.ac.ir/article-1-1309-en.html}, eprint = {http://mlj.goums.ac.ir/article-1-1309-en.pdf}, journal = {Medical Laboratory Journal}, issn = {}, eissn = {2538-4449}, year = {2021} } @article{ author = {Parvizifar, Maryam and Ghanbarzadeh, Mohsen and Shakerian, Saeid and Tavakol, Heshmatollah}, title = {Effect of Short-Term Continuous Aerobic Exercise with Vitamin D Supplementation on high-sensitivity C-reactive protein, Lactate Dehydrogenase and Creatine Kinase in Asthmatic Women}, abstract ={Background and objectives: As a chronic inflammatory airway disease, asthma can increase level of markers of inflammation and muscle damage. In this study, we aimed to evaluate the effects of short-term continuous aerobic exercise combined with vitamin D supplementation on high-sensitivity C-reactive protein (hs-CRP), lactate dehydrogenase (LDH) and creatine kinase (CK) levels in asthmatic women. Methods: In this study, 30 overweight (body mass index=26.97± 1.24 kg/m2) women with mild to moderate asthma were purposefully selected.. The subjects received a vitamin D tablet with a daily dose of 1000 IU for six weeks. The training protocol consisted of two sessions of Balke treadmill test (one session before and one session after the vitamin D supplementation). Blood samples were taken before and immediately after the exercise and before and after vitamin D supplementation. Repeated measures ANOVA was used to evaluate changes in the levels of hs-CRP, CK and LDH. Results: The variables were not significantly affected by the exercise and vitamin D supplementation (P>0.05). Conclusion: It seems that a single session of short-term aerobic exercise and six weeks of vitamin D supplementation do not significantly affect hs-CRP, CK and LDH levels in asthmatic women.}, Keywords = {Exercise,Vitamin D,C-Reactive Protein,}, volume = {15}, Number = {4}, pages = {28-32}, publisher = {school of paramedicine, golestan university of medical sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.52547/mlj.15.4.28}, url = {http://mlj.goums.ac.ir/article-1-1298-en.html}, eprint = {http://mlj.goums.ac.ir/article-1-1298-en.pdf}, journal = {Medical Laboratory Journal}, issn = {}, eissn = {2538-4449}, year = {2021} } @article{ author = {amiri, noosha and Moazzami, Mahtab and Yaghoubi, Ali}, title = {Effect of Eight –Weeks of Resistance Training on Serum Levels of Neurofilament Light Chain and Tau Protein in Women with Multiple Sclerosis}, abstract ={Background and objectives: Multiple sclerosis (MS) is a demyelinating and neurodegenerative disease of the centralnervous system that can be tracked through biomarkers of disease status. The present study aimed to examine effect of eight weeks of resistance training on serum levels of neurofilament light chain and tau protein in women with multiple sclerosis (MS). Methods: The study population consisted of 24 women with MS (aged 25 to 40 years) in Bojnourd (Iran) with expanded disability status scale score of 2-5. Patients were randomly divided into two groups of resistance training (n=12) and control (n=12). The training group performed 45-60 minutes of resistance training, three sessions a week for eight weeks. The control group did not partake in sports activity. Blood samples were taken 24 hours before the first session and 48 hours after the last training session. Analysis of covariance was used to analyze data at a significance level of 0.05. Results: The eight-week resistance training intervention significantly decreased serum level of tau protein but had no significant effect on serum level of neurofilamet light chain. Conclusion: According to the research results, eight weeks of resistance training could have favorable effects on serum level of tau protein in MS patients.}, Keywords = {Resistance training,Tau proteins,Multiple sclerosis,}, volume = {15}, Number = {4}, pages = {33-38}, publisher = {school of paramedicine, golestan university of medical sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.52547/mlj.15.4.33}, url = {http://mlj.goums.ac.ir/article-1-1315-en.html}, eprint = {http://mlj.goums.ac.ir/article-1-1315-en.pdf}, journal = {Medical Laboratory Journal}, issn = {}, eissn = {2538-4449}, year = {2021} } @article{ author = {Khodamoradi, Sepideh and Shahhosseiny, Mohammad Hassan and Mohammadian, Taher and Ferdousi, Atous}, title = {Evaluation of Role of Herpes Simplex Virus Types 1 and 2 and Cytomegalovirus in Alzheimer\'s Disease}, abstract ={Background and objectives: Different environmental factors, such as infection, can cause Alzheimer's disease (AD). Herpes simplex virus types 1 (HSV1) and 2 (HSV2) and cytomegalovirus (CMV) are related to AD. This study explores the potential role of HSV1, HSV2 and CMV in AD progression. Methods: Plasma samples were taken from 100 AD patients (47 women and 53 men). After isolating viral DNA, PCR was performed using specific primers for the detection of the viruses.   Results: The prevalence of CMV, HSV1 and HSV2 was 27%, 8% and 4%, respectively. Although CMV was most prevalent in AD patients, HSV1 and HSV2 were found in patients with advanced AD. The prevalence of HSV1 and HSV2 was significantly associated with dysphoria, hallucination, insomnia and depression (P˂0.05), while CMV was significantly associated with hallucination and dysphoria (P=0.001). AD symptoms were higher in patients with HSV1 and HSV2. Conclusion: It seems that HSV and CMV infections may be related to the severity of AD. }, Keywords = {Alzheimer's disease,Epidemiology,Cytomegalovirus,}, volume = {15}, Number = {4}, pages = {39-44}, publisher = {school of paramedicine, golestan university of medical sciences}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.52547/mlj.15.4.39}, url = {http://mlj.goums.ac.ir/article-1-1347-en.html}, eprint = {http://mlj.goums.ac.ir/article-1-1347-en.pdf}, journal = {Medical Laboratory Journal}, issn = {}, eissn = {2538-4449}, year = {2021} }