eng
school of paramedicine, golestan university of medical sciences
Medical Laboratory Journal
2538-4449
2021-05
15
3
1
6
article
Comparison of Biochemical Compounds of Fertile and Infertile Hydatid Cyst Fluid of Animaland Human Origin
Mohammad Fallah
fallah@umsha.ac.ir
1
Alireza Shiri
alireza.shiri@gmail.com
2
Amir Hossein Maghsood
ahmaghsood@yahoo.com
3
Mohammad Matini
matini.mohammad@yahoo.com
4
Department of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Department of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Department of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Associate Professor, Department of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Background and objectives: Hydatidosis is an important zoonotic disease with widespread distribution. For unknown reasons, some cysts are unable to produce protoscoleces, and little is known about the mechanisms involved in infertile cyst production. Therefore, characterization of hydatid cyst fluid (HCF) components could help clarify the host-parasite relationship and the fertility process of cyst. The aim of this study was to identify and quantify biochemical components of HCF of fertile and infertile cysts from different hosts.
Methods: A total of 35 HCF samples were obtained from the liver and lung of 16 cattle, 16 sheep and three humans. Fertility of cysts was determined by examination of cysts’ content. Then, total fluid was aspirated aseptically from each cyst. The samples were centrifuged at 10000×g for 15 min at 4°C, and then concentrated and dialyzed against phosphate buffer saline using an Amicon Ultra-15 5000 MWCO centrifugal filter device (Millipore, USA). All biochemical components were quantified by an automatic analyzer.
Results: The value of lactate dehydrogenase, potassium, calcium, cholesterol, glucose, urea and uric acid differed significantly in cysts collected from different hosts (P<0.001). There was also a significant difference in the amount of lactate dehydrogenase, aspartate aminotransferase and cholesterol between fertile and infertile cysts (P<0.039).
Conclusion: Due to the differences in biochemical composition of HCF of different host origin, it is possible that the host plays a crucial role in determining the type of biochemistry in hydatid cyst as well as in hydatid cyst fertility.
http://mlj.goums.ac.ir/article-1-1284-en.pdf
Echinococcus granulosus
fertile and infertile hydatid cyst
biochemical compounds
eng
school of paramedicine, golestan university of medical sciences
Medical Laboratory Journal
2538-4449
2021-05
15
3
7
12
article
Effects of Blood Flow Restriction Training on Follistatin Concentrations and Physical Performance in Elderly Females
Farnaz Aminian
fz.aminian@gmail.com
1
Seyyed Mahmood Hejazi
Sm.hejazi37@gmail.com
2
Sadegh Cheragh Birjandi
S_birjandi2001@yahoo.com
3
Department of Exercise Physiology, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran.
Department of Physical Education, Khayyam University, Mashhad, Iran
Department of Exercise Physiology, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran
Background and objectives: Blood flow restriction training (BFRT) has gained substantial interest due to the lower required intensity, which may be beneficial for individuals who are not able to lift heavy weights. Therefore, we aimed at evaluating effects of 12 weeks of resistance training with and without blood flow restriction on follistatin (FST) concentrations and physical performance in elderly females.
Methods: Thirty elderly female were randomly assigned into the following groups: resistance training with blood flow restriction (BFRT; n=10), resistance training without blood flow restriction (WBFRT; n=10) and control ( n=10). The resistance training was carried out three session a week for 12 weeks. Serum concentrations of FST, muscular endurance and dynamic balance were assessed at baseline and after the 12week intervention.
Results: Significant main effects of time were observed for FST (p =0.03, η2 = 0.15), muscular endurance (p = 0.00, η2 = 0.59) and dynamic balance (p=0.00, η2 = 0.57). FST [BFRT= 1.4 ng/ml (effect size Cohen’s {d} = -0.8) significantly increased only in BFRT group. However, muscular endurance [BFRT= 95 (d= -4.1) and WBFRT = 32 (d= -0.9)] significantly increased in both intervention groups (P<0.05). In addition, dynamic balance [BFRT= -0.5 seconds (d= 2.2)] significantly increased only in the BFRT group (P<0.05).
Conclusion: BFRT was able to increase FST concentrations. Due to its mechanistic role in muscle mass alterations, elderly females can incorporate our BFRT protocol to improve anabolic conditions for muscular adaptations.
http://mlj.goums.ac.ir/article-1-1295-en.pdf
Resistance training
Follistatin
Aged
Female
eng
school of paramedicine, golestan university of medical sciences
Medical Laboratory Journal
2538-4449
2021-05
15
3
13
20
article
Antimicrobial Effect of Gel-Type Nanoemulsion of Chitosan Coating Containing Essential Oils of Zataria multiflora and Bunium persicum on Pseudomonas Artificially Inoculated onto Salmon Fillets
mehrdad rezaeian
mehrdaderezaeian@gmail.com
1
Saeid Khanzadi
khanzadi@um.ac.ir
2
Mohammad Hashemi
3
Mohammad Azizzadeh
4
Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran c Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Background and objectives: Chitosan is a preservative that is commonly used in food packaging due to forming a film with antimicrobial activity. Many antimicrobial agents have been used to control the growth of different bacteria, fungi and yeasts in food products using chitosan coating. The present research was conducted to examine inhibitory effects of a coating incorporated with the essential oils of Zataria multiflora (ZEO) and Bunium persicum (BEO) on the growth of Pseudomonas artificially inoculated onto salmon fillets over a period of 12 days at 4 °C.
Methods: The antibacterial activity of BEO against P. aeruginosa was evaluated using the microdilution method via determining minimum inhibitory concentration and minimum bactericidal concentration. For the food model investigation, three P. aeruginosa strains were inoculated onto trout fillets as culture cocktail to assess their survival over 12 days of storage.
Results: The results indicated that ZEO and BEO had stronger inhibitory effect on P. aeruginosa in trout fillets when applied along with gel type nano-emulsion of chitosan solution. The separate use of each of these substances also significantly inhibited the growth of these pathogenic bacteria compared with the control. In addition, the use of chitosan coating without any antimicrobial agent affected the growth of P. aeruginosa.
Conclusion: The gel type nano-emulsion of chitosan coating containing ZEO and BEO can be applied on foodstuff, particularly fish and its products, as an antimicrobial agent.
http://mlj.goums.ac.ir/article-1-1277-en.pdf
Fishes
Chitosan
Pseudomonas aeruginosa
eng
school of paramedicine, golestan university of medical sciences
Medical Laboratory Journal
2538-4449
2021-05
15
3
21
26
article
Effect of Six Weeks of Aerobic Interval training on Serum Vaspin and Blood Glucose Levels in Obese Inactive Girls
Marzeyeh Mirzazadeh Bakhteyari
m.mirzazade.114@gmail.com
1
saied shakerian
sashakeryan@gmail.com
2
mohsen ghanbarzadeh
ghanbarzadeh۲۱۳@gmail.com
3
Faculty of Sports Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Department of Exercise Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Department of Exercise Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Background and objectives: The prevalence of obesity and overweight in women is increasing. Secretion of adipokines such as vaspin may affect insulin sensitivity. It has been suggested that physical activity can alter circulating vaspin levels. The purpose of this study was to investigate effects of six weeks of aerobic interval training on serum vaspin and blood glucose levels in obese inactive girls.
Methods: In this quasi-experimental study, 22 inactive overweight girls were selected from the Shahid Chamran University of Ahvaz, Iran. The mean age, weight and body mass index of the subjects were 24.22±1.35 years, 83.77±12.66 kg and 32.56±3.36 kg/m2, respectively. The subjects were randomly divided into an experimental group )n=11) and a control group )n=11). The experimental group performed aerobic training on stationary bicycle at 65-80% of maximal strength, three sessions a week for six weeks. Serum levels of vaspin and glucose were measured using commercial kits. Data were analyzed using SPSS (version 23) and at significance of 0.05.
Results: The training intervention had no significant impact on the serum vaspin and glucose concentrations (P>0.05). In addition, the covariance analysis showed that serum vaspin and glucose levels did not differ significantly between the study groups (P>0.05).
Conclusion: Based on the results, the six-week aerobic interval training had no significant effect on vaspin and blood glucose levels of inactive obese girls. It is recommended to further examine the effect of this type of exercise at higher intensities and for longer durations.
http://mlj.goums.ac.ir/article-1-1305-en.pdf
Blood Glucose
High-Intensity Interval Training
Women
eng
school of paramedicine, golestan university of medical sciences
Medical Laboratory Journal
2538-4449
2021-05
15
3
27
32
article
Investigating the Protective Effects of Endurance and Resistance Trainings on Serum level of Cellular and Vascular Adhesive Molecules in Obese Men
Mohammad Hossein Akha
akha.mohammad@gmail.com
1
Seyed Abdollah Hashemvarzi
Hashemvarzi_tkd@yahoo.com
2
Department of Sports Physiology, Sari Branch, Islamic Azad University, Sari, Iran
Department of Sports Physiology, Sari Branch, Islamic Azad University, Sari, Iran
Background and objectives: The aim of this study was to compare the effect of an eight-weeks endurance and strength training on levels of cellular and vascular adhesion molecules in obese men.
Methods: Twenty-four obese volunteer men as subjects in this study were selected from Tehran and randomly assigned to control, endurance training, and strength training groups. The enduranBackground and objectives: Obesity, particularly abdominal obesity, is the most common cause of metabolic abnormalities, such as metabolic syndrome. The aim of this study was to compare effects of eight weeks of endurance and strength trainings on serum levels of cellular and vascular adhesion molecules in obese men.
Methods: Twenty-four obese men were randomly assigned to control, endurance training and strength training groups. The endurance training group performed a modified treadmill running protocol with an intensity of 50-70% of maximum heart rate. The resistance training included chest press, armpit stretch and leg and crus presses at intensity of 50-80% of one-repetition maximum. Serum levels of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion protein 1 (VCAM--1) were measured using commercial ELISA kits. Data were analyzed by one-way ANOVA and post hoc Tukey test at significance of 0.05.
Results: Serum VCAM-1 and ICAM-1 levels decreased significantly in both training groups compared to the control group (P=0.0001). The reduction of serum VCAM-1 and ICAM-1 levels was more significant in the endurance training group than in the resistance training group.
Conclusion: Given the favorable effects of endurance and resistance trainings on serum levels of VCAM-1 and ICAM-1, the eight-week training could be performed as a protective intervention in obese people.ce training group performed a modified running protocol with an exercise intensity of 50% to 70% of HRmax on the treadmill. Resistance exercises also included chest press, armpit stretch, and leg and crus presses. The training intensity was 50% to 80% of 1-RM. Serum levels of VCAM-1 and ICAM-1 were measured using ELISA commercial kits. Data were analyzed by One-way ANOVA and post hoc Tukey at the significant level P<0.05.
Results: VCAM-1 and ICAM-1 levels decreased significantly in training groups compared to the control (P = 0.0001). Significant differences were also found between the values of VCAM-1 and ICAM-1 in the endurance training group compared to the resistance training group (P = 0.0001).
Conclusion: Overall, the levels of molecules showed that the values of VCAM-1 and ICAM-1 decreased significantly in the exercise group compared to the control group, but this decrease was more significant in the endurance training group. Therefore, it seems that 8 weeks of training in obese people can be very useful and prevent many diseases.
http://mlj.goums.ac.ir/article-1-1300-en.pdf
Endurance Training
Resistance Training
Intercellular Adhesion Molecule-1
Vascular Cell Adhesion Molecule-1
Obesity
eng
school of paramedicine, golestan university of medical sciences
Medical Laboratory Journal
2538-4449
2021-05
15
3
33
39
article
Evaluation of Changes in Insulin Resistance and Serum Cortisol Levels after Eight Weeks of Continuous and Interval Aerobic Training in Healthy and Obese Girls
Ameneh Bahreini
a_bahreine2@yahoo.com
1
Maryam Akbarpour
art635@yahoo.com
2
Rozita Fathi
roz_fathi@yahoo.com
3
Hossein Goldashti
h_goldashti@yahoo.com
4
Department of physical Education, Islamic Azad University, Chalous Branch, Iran
Department of Cultivation and Plant Breeding, Faculty of Agriculture and Natural Resources, Islamic Azad University, Chalous Branch, Iran
Department of sport physiology, Mazandaran University, Babolsar,Iran
Department of Physical Education, Islamic Azad University, Chalous Branch, Iran
Background and objectives: Given the growing prevalence of obesity in children, the present study aimed to investigate effects of continuous and interval aerobic training on insulin resistance index and serum cortisol levels of obese girls.
Methods: The study was performed on 36 healthy girls aged 9-11 years with a body mass index (BMI) of higher than 85th percentile. The subjects were randomly divided into three groups of interval training, continuous training and control. The training groups performed exercises three days a week for eight weeks. The exercises were performed at 70% of VO2max in the first four weeks and at 80% of VO2max in the following weeks. Fasting blood samples were taken 72 hours before the first session and 72 hours after the last training session in order to measure levels of cortisol, glucose, insulin and Homeostatic Model Assessment for Insulin Resistance (HOMA-IR).Weight, BMI and body fat percentage were also measured by conventional methods. Data analysis was performed in SPSS 21 using paired t-test and one-way analysis of variance.
Results: The mean level of BMI and body fat percentage decreased significantly after the interval and continuous training, respectively (P<0.05). The HOMA-IR index did not change significantly in the study groups (P>0.05). The cortisol level increased slightly in the continuous training and control groups (P=0.075) and decreased insignificantly (P=0.131) in the interval training group. There was no difference between the training groups and the control group in terms of HOMA-IR and cortisol levels.
Conclusion: Insulin levels, HOMA-IR showed a decrease trend while a reasonable increase in cortisol level we showed in the continuous training group. BMI level and fat percentage decreased in two both training groups.
http://mlj.goums.ac.ir/article-1-1297-en.pdf
Continuous training
Interval training
Insulin Resistance
Cortisol
Obese children
eng
school of paramedicine, golestan university of medical sciences
Medical Laboratory Journal
2538-4449
2021-05
15
3
40
46
article
Effects of Aerobic Exercise and Ethanolic Extract of Purslane Seed on Markers of Oxidative Stress and DNA Damage in Cardiac Tissue of Rats Poisoned with Hydrogen Peroxide
shiva bahram vash shams
1
parvin farzanegi
parvin.farzanegi@gmail.com
2
Mohammad Ali Azarbayjany
3
MSc Student of Exercise Physiology, Department of exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Associate Professor of Exercise Physiology, Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran
Professor of Exercise Physiology, Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Background and objectives: Oxidative stress is the most important pathophysiological cause of diseases. Studies have shown that exercise and supplementation with medicinal plants have limited side effects. The aim of the present study was to evaluate effects of aerobic exercise and ethanolic extract of purslane seed on ATP, O-6-Methylguanine DNA methyltransferase (MGMT), malondialdehyde (MDA) and prooxidant-antioxidant balance (PAB) levels in the heart tissue of rats poisoned with hydrogen peroxide.
Methods: In this experimental trial, 72 male Wistar rats were randomly divided into nine groups: (1) control + H2O2 , (2) aerobic exercise, (3) aerobic exercise and 50 mg/kg purslane seed extract, (4) aerobic exercise and 200 mg/kg purslane seed extract, (5) aerobic exercise and 400 mg/kg purslane seed extract, (6) 50 mg/kg purslane seed extract, (7) 200 mg/kg purslane seed extract, (8) 400 mg/kg purslane seed extract, and (9) healthy control. Oxidative stress was induced by intraperitoneal injection of 1 mmol/kg hydrogen peroxide three times a week for eight weeks. Aerobic exercise was performed three sessions a week for eight weeks, and the purslane seed extract was intraperitoneally injected daily at the mentioned doses.
Results: Aerobic exercise and purslane seed extract alone or combined significantly increased ATP, MGMT and significantly reduced MDA and PAB levels in cardiac tissue of rats exposed to hydrogen peroxide (P<0.05). Moreover, the effect of purslane seed extract was dose dependent.
Conclusion: It seems that aerobic exercise and purslane seed extract supplementation have synergistic cardioprotective effects under oxidative stress.
http://mlj.goums.ac.ir/article-1-1258-en.pdf
Exercise
Purslane Seed
MGMT
ATP
MDA
PAB
Heart
eng
school of paramedicine, golestan university of medical sciences
Medical Laboratory Journal
2538-4449
2021-05
15
3
47
50
article
Soil Contamination with Eggs of Toxocara spp. in Yazd, Central Iran
Mohammad-Ali Mohaghegh
mohaghegh1982@yahoo.com
1
Roghayeh Norouzi
roghayehnorouzi123@gmail.com
2
Abolghasem Siyadatpanah
asiyadatpanah@yahoo.com
3
Farzaneh Mirzaei
mirzaei.farzaneh2015@yahoo.com
4
Ali Fatahi bafghi
afbafghi@ssu.ac.ir
5
Seyed-Reza Mirbadie
Mirbadie@gmail.com
6
Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Ferdows Paramedical School, Birjand University of Medical Sciences, Birjand, Iran
Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
Background and objectives: Soil contamination with Toxocara spp. eggs is the main source of human toxocariasis. The aim of this study was to determine rate of soil contamination with Toxocara spp. eggs in Yazd, central Iran.
Methods: During September 2014 to February 2015, a total of 220 soil samples were collected from public parks, primary schools, green areas, kindergartens, suburban areas, streets, residential complexes, backyards and a bus terminal in the city of Yazd, Yazd Province. Soil samples were investigated for the presences of Toxocara eggs by flotation technique using sucrose solution (1.2 g/cm3) and using light microscopy. Data analysis was carried out by SPSS (version 23) software package.
Results: A total of 220 soil samples from nine different places were examined. Of these samples, 51 (23.2%) were contaminated with Toxocara spp. eggs. The contamination rate was 26.3% in public parks, 19.2% in primary schools, 28.6% in green areas, 22.2% in kindergartens, 24% in suburban areas, 8.3% in streets, 18.8% in residential complexes, 19% in backyards and 28.6% in the bus terminal. There was no significant difference in the soil contamination rate with Toxocara spp. eggs between different sampling places (χ2 = 3.096, d.f = 8 and P= 0.92) and sampling areas (χ2= 2.289, d.f = 2 and P= 0.318).
Conclusion: The high rate of soil contamination with Toxocara spp. eggs in Yazd emphasize on the need for preventive measures, such as improving sanitation, providing public health education and controlling stray dogs and cats near children play areas.
http://mlj.goums.ac.ir/article-1-1285-en.pdf
Toxocara
Environmental Pollution
Yazd
Iran