Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1395
4
1
gregorian
2016
7
1
10
4
online
1
fulltext
fa
Comparative Evaluation of Chemical Reduction Methods by Ethanol and Bacillus licheniformis on The Physical Properties, Stability and Coating of Nanoparticles
تحقيقي
Original Paper
<div>
<p><strong>ABSTRACT</strong></p>
<p><strong> Background and Objectives</strong>: Size of silver nanoparticles synthesized by ethanol and <em>Bacillus lichenioformis</em>is 20 nm and 15 nm, respectively. Nanoparticles can be used in treatment of several diseases. Chemical and biological methods have been used to synthesize silver nanoparticles. The aim of this study was to compare the size, shape and coating of silver nanoparticles synthesized by the chemical and biological methods.</p>
<p><strong> Methods:</strong> Ethanol was used in the chemical reduction method and <em>B. licheniformis</em> was used in the biological method. Physical evaluation (salt test), absorbance measurement at 450 nm and imaging by transmission electron microscopy were performed to compare nanoparticles in terms of size, shape and coating.</p>
<p><strong> Results:</strong> Observed maroon color, maximum absorption at 400-450 nm range and electron microscopy images confirmed the presence of nanoparticles. The shape of nanoparticles synthesized by the two methods was spherical. However, biosynthesized nanoparticles were smaller and had protein coating.</p>
<p><strong> Conclusion:</strong> Given the smaller size of biosynthesized nanoparticles and presence of coating confirmed by the electron microscopy images, biosynthesis is recommended because of enhanced nanoparticles properties and reduced toxicity.</p>
<p><strong> Keywords:</strong> Nanoparticles, Coating, Toxicity.</p>
</div>
Nanoparticles, Coating, Toxicity.
53
57
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-415&slc_lang=fa&sid=1
Shima
Doostmohammadi
shima.dmohammadi@yahoo.com
100319475328460011083
100319475328460011083
Yes
Department of Microbiology, Faculty of Science, Islamic Azad University, Science and Research Branch, Sirjan, Iran
Babak
Kheirkhah
100319475328460011084
100319475328460011084
No
Department of Microbiology, Faculty of Science, Islamic Azad University, Kerman Branch, Kerman, Iran
Seyed Mohammad Reza
Khoshroo
100319475328460011085
100319475328460011085
No
Department of Microbiology, Faculty of Science, Islamic Azad University, Kerman Branch, Kerman, Iran