Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1394
12
1
gregorian
2016
3
1
10
2
online
1
fulltext
fa
Sequence Analysis of HSP70 Gene of Leishmania major and Leishmania tropica in Chabahar and Mashhad
تحقيقي
Original Paper
<div>
<p><strong>ABSTRACT</strong></p>
<p><strong> Background and Objective:</strong> Cutaneous leishmaniasis is a parasitic disease and a health problem in different parts of Iran, especially two cities of Mashhad and Chabahar. Due to morphological similarities of most <em>Leishmania</em> species and difference in reservoirs of <em>L. major</em> and <em>L. tropica</em>, it is necessary to determine the parasite specie to combat the disease. Thus, this study used gene sequencing and genotyping of 70-kDa heat shock protein (HSP70) to differentiate the two species of <em>Leishmania</em>.</p>
<p><strong> Methods:</strong> In this descriptive-analytical study, microscope slides and cultures were prepared from 43 patients suspected of cutaneous leishmaniasis in Chabahar and Mashhad. PCR was performed after genomic DNA extraction and then PCR products were sequenced and analyzed.</p>
<p><strong> Results:</strong> Of the 43 patients studied, 32 direct smear and culture (74.4%) were positive and 11 (25.6%) showed negative results, and were therefore excluded from the study. Using HSP70-specific primers, 1962 bp and 1152bp bands were observed for HSP70 of <em>L. major</em> in Chabahar and <em>L. tropica</em> in Mashhad, respectively. Based on the results, there were 18 nucleotide differences between HSP70 of <em>L. major</em> in Chabahar and <em>L. tropica</em> in Mashhad.</p>
<p><strong> Conclusion:</strong> Due to the morphological similarities between <em>Leishmania</em> species and inability to differentiate species through parasitological methods, the HSP70 gene can be used for identification of the species, and prevention and treatment of the disease.</p>
<p><strong> </strong></p>
</div>
HSP70 Gene, Leishmania major, Leishmania tropica, Chabahar, Mashhad.
41
45
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-393&slc_lang=fa&sid=1
Mansour
Dabirzadeh
abbaspashaie41@gmail.com
100319475328460011346
100319475328460011346
No
School of Medicine, Zabol University of Medical Sciences, Zabol, Iran
Abbas
Pashaie Neghadeh
abbaspashaie41@gmail.com
100319475328460011347
100319475328460011347
Yes
School of Medicine, Zabol University of Medical Sciences, Zabol, Iran
Tahere
Davoodi
100319475328460011348
100319475328460011348
No
School of Medicine, Zabol University of Medical Sciences, Zabol, Iran
Mohammad
Hashemi
100319475328460011349
100319475328460011349
No
School of Medicine, Zabol University of Medical Sciences, Zabol, Iran