Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1386
1
1
gregorian
2007
4
1
1
1
online
1
fulltext
fa
مقایسه سطح سرمی آلفا-1-آنتی تریپسین (AAT) با سه روش آنزیمی، الکتروفورز و ایمونودیفیوژن
Serum Alpha-1-antitrypsin Evaluation by Three Electrophoretic, Enzymatic and Immunodiffussion Methods
<p align="justify" style="DIRECTION: ltr">Abstract Background & Objective: Alpha-1-antitrypsin (AAT) is the major component of the human plasma alpha-1 globulin proteins and acts as a major inhibitor of proteolytic enzymes, particularly elastase. AAT deficiency is accompanied by lung, liver and other disorders, therefore, AAT is clinically important and its precise evaluation is diagnostically critical. In present study serum AAT was evaluated by three Cellulose Acetate Electrophoresis (CAE), Trypsin Inhibitory Capacity (TIC) and Single Radial Immunodiffusion (SRID) methods and results wene compared. Materials and methods: AAT evaluation was carried out, by CAE, SRID and TIC Methods, on 318 normal sera obtained from volunteer students of Tehran Universities. Results:The results indicated: 34, 84 and 112 samples by TIC, SRID and CAE methods (with reference ranges of 2.1-3.5 mol/min/ml, 126- 226 mg/dl and 2-4.5% respectively) were abnormal 201samples by CAE and TIC were normal and 29 abnomal, 83 sera were normal by TIC and abnormal by CAE five of them were abnormal by TIC and normal by CAE 227 of the samples were normal and 29 abnormal(TIC and SRID) 57 were normal by TIC and abnormal by SRID and seven samples were abnormal by TIC and normal by SRID. Conclusion: Although CAE and alpha-1 globulin band determination are routine in clinical laboratory, they are not reliable in evaluating AAT. SRID sensitivity is more Than CAE and less Than TIC therefore, TIC is recommended as a precise and reliable method for serum AAT evaluation. Key Words: Alpha-1-antitrypsin, Cellulose Acetate Electrophoresis, Single Radial Immunodiffusion, Trypsin Inhibitory Capacity</p>
Key Words: Alpha-1-antitrypsin, Cellulose Acetate Electrophoresis,Single Radial Immunodiffusion, Trypsin Inhibitory Capacity
0
0
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-76&slc_lang=fa&sid=1
M
Mohammadian Yajloo,
محسن
محمدیان یاجلو
mohammadian@razitums.ac.ir
100319475328460012972
100319475328460012972
Yes
دانشکده بهداشت و انستیتو تحقیقات بهداشتی دانشگاه علوم پزشکی تهران
A
Sahebgadam Lotfi
عباس
صاحبقدم لطفی
100319475328460012973
100319475328460012973
No
دانشگاه علوم پزشکی تربیت مدرس
M
Nasroallahzadeh Sabet
مهرداد
نصراله زاده ثابت
100319475328460012974
100319475328460012974
No
دانشگاه علوم پزشکی تهران
N
Zhalehjoo
نغمه
ژاله جو
100319475328460012975
100319475328460012975
No
دانشگاه علوم پزشکی ایران
M
Amirian
مرضیه
امیریان
100319475328460012976
100319475328460012976
No
مرکز تربیت معلم کرمانشاه
M
Biglarzadeh
مریم
بیگلرزاده
100319475328460012977
100319475328460012977
No
دانشگاه علوم پزشکی تهران