Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1399
2
1
gregorian
2020
5
1
14
3
online
1
fulltext
en
Isolation, identification, and antibacterial susceptibility testing of Moraxella catarrhalis isolated from the respiratory system of patients in northern Iran
باکتری شناسی
bacteriology
تحقيقي
Original Paper
<div><strong>Background and Objectives: </strong>Moraxella catarrhalis is considered as an emerging pathogen and a new nosocomial infection agent. This study was conducted to isolate and identify M. catarrhalis from clinical samples (respiratory tracts) and assess them for antimicrobial susceptibility patterns.<br>
<strong> Methods: </strong>In total, 280 samples were collected from patients with respiratory tract infection, and 120 samples were obtained from healthy individuals in the control group. The isolates were identified by phenotyping and genotyping methods, and their antibiotic susceptibility was evaluated using disk diffusion methods. The presence of β-lactamase and efflux pump activity were specified via phenotypic methods. Finally, Bro and acrA genes in the isolates were detected by PCR technique.<br>
<strong> Results: </strong>The frequency of this bacterium was 9.64% (27 out of 280) in patients with respiratory tract infection and 4.16% (5 out of 120) in the control group. Although the isolates were resistant to penicillin, they had various responses against other antibiotics. The results obtained from molecular method showed that 90.6% and 84.3% of the isolates possessed Bro and acrA genes, respectively. There was a significant relationship (P<0.05) between the presence of Bro and acrA genes and antibacterial resistance to ampicillin, amoxicillin, cefazolin, cefuroxime, and chloramphenicol.<br>
<strong> Conclusion: </strong>Our findings confirmed the existence of M. catarrhalis in patients with respiratory diseases and the high prevalence of antibiotic resistant genes in M. catarrhalis isolates. Therefore, timely diagnosis and successful treatment can play important roles in preventing their spread.</div>
Moraxella Catarrhalis, Respiratory Tract Infection, Drug Resistance, Β-Lactamase
19
25
http://mlj.goums.ac.ir/browse.php?a_code=A-10-837-1&slc_lang=en&sid=1
Mina
Eghbali
minaeghbali@gmail.com
100319475328460017448
100319475328460017448
No
1. Department of Microbiology, Fars Science and Research Branch, Islamic Azad University, Marvdasht, Iran 2. Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
Majid
Baserisalehi
majidbaserisalehi@gmail.com
100319475328460017449
100319475328460017449
Yes
Department of Microbiology, Kazeron Branch, Islamic Azad University, Kazeron, Iran.
Masood
Ghane
masoodghane@GMAIL.COM
100319475328460017450
100319475328460017450
No
4. Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.