Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1397
10
1
gregorian
2019
1
1
13
1
online
1
fulltext
fa
Protective Effect of Ilex spinigera and Gleditsia caspica Extracts against Drug-Induced Hemolysis in Glucose-6-phosphate dehydrogenase-deficient Patients
تحقيقي
Original Paper
<div><strong>ABSTRACT</strong><br>
<strong> Background and Objectives</strong>: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common X-linked disorder of human erythrocytes in which cells are susceptible towards hemolytic changes and could be destroyed by peroxides. Extracts of <em>Ilex spinigera</em> and <em>Gleditsia caspica</em> leaves have excellent free radical scavenging activity. We investigated the protective effects of these extracts against hemolysis induced by some drugs in G6PD-deficient erythrocytes.<br>
<strong> Methods</strong>: Blood samples were collected from males with and without G6PD deficiency. Hemolysis induced by aspirin, phenylhydrazine hydrochloride and phenacetin was assessed in the presence and absence of the extracts. The amount of released hemoglobin was determined by reading absorbance at 540 nm.<br>
<strong> Results: </strong>The methanol extract of <em>G. caspica</em> had significant protective effects against phenacetin-induced hemolysis in G6PD-deficient human erythrocytes (P<0.05). However, the <em>I. spinigera</em> extract had no significant anti-hemolytic effects on these cells.<br>
<strong> Conclusion:</strong> Our findings suggest that the extract of <em>G. caspica</em> could be a potential drug with antioxidant and anti-hemolytic properties for patients with G6PD deficiency.<span dir="RTL"></span><br>
<strong> Keywords:</strong> Antihemolytic activity, Medicinal plant, G6PD deficiency, <em>Gleditsia caspica</em>,<em> Ilex spinigera. </em></div>
Antihemolytic activity, Medicinal plant, G6PD deficiency, Gleditsia caspica, Ilex spinigera.
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http://mlj.goums.ac.ir/browse.php?a_code=A-10-636-2&slc_lang=fa&sid=1
Maryam
Mohadjerani
100319475328460014968
100319475328460014968
Yes
Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Mansoore
Damanjany
100319475328460014969
100319475328460014969
No
Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran