RT - Journal Article T1 - Evaluation of miR-101 Level in Patients with Chronic Hepatitis B Virus Infection and Liver Cirrhosis JF - mljgoums YR - 2017 JO - mljgoums VO - 11 IS - 3 UR - http://mlj.goums.ac.ir/article-1-977-en.html SP - 10 EP - 14 K1 - MicroRNAs K1 - Chronic Hepatitis B K1 - Liver Cirrhosis K1 - MiR-101. AB - ABSTRACT Background and Objective: MiRNAs are small RNAs that are expressed in most eukaryotes, and can regulate gene expression by attaching to the 3’ end of target mRNA. MicroRNA-101 (miR-101) post-transcriptional regulation is important for host-virus interactions. In addition, miR-101 has a tumor suppressive role in liver cancer and metastasis, and induces apoptosis in tumor cells. We examined miR-101 expression in patients with chronic hepatitis B, hepatitis B virus (HBV)-associated cirrhosis and healthy individuals. Methods: The study was performed on 108 whole blood samples (36 samples from each group) collected in EDTA tubes. RNA was extraction by RNX-plus kit according to the manufacturer’s protocol. Finally, miRNA expression was evaluated using relative real time PCR. Results: A 2.4-fold increase was observed in miR-101 expression in patients with chronic hepatitis B, while there was a 3.5-fold increase in miR-101 expression in patients with HBV-associated cirrhosis compared with healthy controls (P=0.003). MiR-101 overexpression in patients with HBV-associated cirrhosis was more notable that in patients with chronic hepatitis B. Conclusion: According to the results, evaluating miR-101 expression may predict disease progression from chronic hepatitis B to HBV-associated cirrhosis. Keywords: MicroRNAs, Chronic Hepatitis B, Liver Cirrhosis, MiR-101. LA eng UL http://mlj.goums.ac.ir/article-1-977-en.html M3 10.18869/acadpub.mlj.11.3.10 ER -