Aliehsan Karshenas, Ramak Yahya Raiat, Taghi Zahraiee Salehi, Babak Asghari, Maryam Adabi,
Volume 18, Issue 2 (3-2024)
Abstract
Background: Escherichia coli consists of a wide range of strains with huge diversity in their genome, distributed in nature and the alimentary tracts of animals and humans. This study analyzed the phylogenetic group determination and genetic diversity of E. coli strains isolated from domestic animals and human clinical samples.
Methods: Twenty E. coli isolates from domestic animals were analyzed for phylogenetic grouping. Also, 100 clinical samples and 20 animal samples were evaluated by the enterobacterial repetitive intergenic consensus–polymerase chain reaction (ERIC-PCR) technique. The results and the similarity between the strains were determined based on the Dice similarity coefficient in the SAHN program of the NTSYS-pc software.
Results: The frequency of phylogroups among animal samples were A = 5%, B1 = 65%, B2 = 20%, and D = 10%. Based on the ERIC-PCR results, the clinical strains were allocated into 19 clusters. Most strains were in the E7 cluster. Fifty percent of the E. coli isolated from animal specimens belonged to the E4 group, and the lowest number of strains was in the E3 and E5 (1 strain) groups.
Conclusion: The results confirmed the efficiency and usefulness of the ERIC-PCR tool for the identification and classification of bacteria. Also, we demonstrated the most phylogroup among animal samples.
Bahman Aghcheli , Romina Yavarinamini , Alireza Tahamtan ,
Volume 20, Issue 1 (1-2026)
Abstract
Background: Severe lower respiratory tract infections in infants and young children are frequently caused by respiratory syncytial virus (RSV), with the degree of illness strongly associated with disproportionate inflammatory activity. The signaling protein A20 (TNFAIP3) functions to inhibit NF-κB pathway activation, suggesting a possible role in tempering RSV-triggered lung inflammation. In this study, we assessed how RSV infection alters A20 gene expression in the lungs using a mouse model system.
Methods: Of the twelve female BALB/c mice allocated for the study, half were administered RSV intranasally at a concentration of 3 × 106 plaque-forming units (PFU), while the remaining six served as uninfected controls. All animals were humanely euthanized five days post-infection. Upon collection, lung tissue samples were immediately processed. The relative expression levels of messenger RNA (mRNA) for the TNFAIP3 gene, which encodes the A20 protein, were subsequently quantified using real-time reverse transcription polymerase chain reaction (RT-PCR).
Results: Analysis by quantitative PCR revealed that A20 expression was significantly higher in the lungs of RSV-infected mice compared with uninfected controls at day 5 post-infection (P = 0.0048).
Conclusion: The upregulation of A20 in RSV-infected mice suggests its potential role in modulating post-viral pulmonary inflammation.
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