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Soltan Dallal. M.m, Rahimi Forushani, A., Sadigh Maroufi, S, Sharifi Yazdi, K,
Volume 5, Issue 2 (10-2011)
Abstract

Abstract Bachground and objectives: Salmonella is one of the most important agents of gastrointestinal infection and diarrhea in our country. Misdiagnosis of these bacteria leads to cure failure. The aim of this study was to make a comparison between PCR and the API-20E and conventional biochemical tests carried out for the identification of Salmonella. Material and Methods: In this study 470 specimens taken from children, with acute gastroenteritis, referred to teaching hospitals called Imam, Shariati and children medical centre. The specimens were transferred to microbiology laboratory in public health school for identification of Salmonella with PCR and API-20E methods. Results: Of 470 specimens, 65(13.8%) are positive for salmonella in hospital laboratory, while 37 (7.9%) for API-20E and 39 (8.3%) for PCR are positive. The results of antibiotic sensitivity tests on 39 salmonella isolated from diarrhea specimens show that 73.3% of them are resistance to at least one of the sixteen antibiotics tested. Conclusion: Based on the the results, there is significant difference (P<0.05) between conventional method, API-20E and PCR Key words: Salmonella, conventional identification, molecular identification
Soltan Dallal Mm, Rahimi Forushani A, Bakhtiari R,
Volume 6, Issue 1 (4-2012)
Abstract

Abstract Background and objectives: Helicobacter pylori is a helical gram negative bacterium with polar flagella, discovered by Warren and Marshall in 1983. Helicobacter pylori exist in the stomach mucus tissue of less than 20% of people under 30 years old, but this amount would increase up to 40% and 60% in 60- year- old people. The aim of this study was to compare three methods of culture media, direct slide staining and the urease test for the rapid diagnosis of bacterium in case of peptic or duodenal ulcer. Material and Methods: In This descriptive study, duplicate biopsy specimens were taken from 82 clients referring to four different Hospitals .In endoscopy room of the Hospitals, a rapid urease test were carried out on one of duplicate specimens for the presence or non-presence of Helicobacter pylori. In order to see the Helicobacter pylori in the tissues, three slides using foushin, giemsa, and gram staining were prepared from the second specimens. Then, the specimens were incubated into selective culture media and incubated for 4-6 days in micoraerophilic condition. Results: Of 82 tested specimens 70(85.5%) and 66(80.5%) are identified as Helicobacter pylori by positive urease and culture medium, respectively. The frequency of foushin, giemsa, and gram staining are 67 (81.7%), 66 (80.5%), and 61 (74.4%), respectively. The foushin staining is the best with 100% sensitivity among the other methods. Conclusion: Based on difference between proportions, There is no significant difference between staining methods (foshin, giemsa, gram staining) and culture media in all cases. Key words: Helicobacter pylori, microscopic methods, urease test, culture media, identification
Maedeh Kiani Abri , Monir Doudi , Ali Mohammad Ahadi ,
Volume 12, Issue 3 (5-2018)
Abstract

ABSTRACT
          Background and Objectives: Keratinase is an enzyme commonly used in the production of detergents, cosmetics, drugs, leather, and other industries. Considering the high cost of traditional methods for decomposition of feather, hair, hooves, nails, and wool that contain high levels of keratin, their biodegradation with keratinase-producing bacteria can be a valuable solution. The present study aimed for isolation and molecular identification of keratinase-producing bacteria in Qeshm Island and Peyposht village in Iran.
          Methods: Water and sludge samples from the Qeshm Island and Peyposht village were collected. The bacteria isolates were screened for keratinase production using the Lowry method. Effect of pH and temperature was assessed on the production of keratinase and on the growth of the isolates. Colony-polymerase chain reaction was used for molecular identification of the isolates.
          Results: Bacillus berevis and Enterobacter cloacae were isolated in this study. Keratinase production in B. berevis was highest at pH 7.5 and 35 °C. In addition, the highest level of enzyme production by E. cloacae was observed at pH 7 and 37 °C.
          Conclusion: It seems that the bacterial strains isolated from sludge in the study area have relatively favorable keratinase production capacity.
          Keywords: Bacteria, Colony PCR, Identification, Keratinolytic protein, Sewage.
 
 


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