Showing 4 results for Ziaei
N Ziaei, N Amir Mozafari, H Kouhsari, A Moradi, A Tabarai, T Dadgar, S Livani, M Arab Ahmadi,
Volume 2, Issue 2 (Autumn – Winter 2009[PERSIAN] 2008)
Abstract
Abstract Background and Objectives: Diarrhea is one of the most common diseases in the world. Campylobacter jejuni is one of the prevalent agents of bacterial diarrhea in most of developing countries. It is usually ignored in routine laboratory test in our country, because it has a difficult investigation method. This article aims to determine the prevalence of Campylobacter jejuni, in diarrhea samples in Gorgan City (East north of Iran) by PCR Method. Material and Methods: This descriptive study was performed on 455 diarrheal samples during one year (2005-06).255 out of them were cultured on Preston media (Himedia co.) on 42°c. DNA Extracted by phenol cholorophorm method was directly carried out on stool samples.16srDNA hipo and asp primers for detection of Campylobacter genus, C.jejuni and C.coli species were used, respectively. In addition, universal primer of 16srDNA was used for control of PCR method. Results: no sample was positive for Campylobacter in culture .only three samples were positive for Campylobacter genus and C.jejuni specific primer but none of them were positive for C.coli .99 samples were positive by universal primer of 16srDNA . Conclusion: The results indicate that C.jejuni isn't a prevalent agent in diarrhea in our region. Key words: Campylobacter jejuni -Gorgan- Diarrhea
Ar Niazi, F Koohsar, F Ghaffarifar, H Ziaei-Hezarjaribi,, F Mesgarian, on Jorjani,
Volume 8, Issue 2 (summer 2014[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: Culture, microscopic method is a gold standard method for identification of Lishmania parasite. The use of Molecular methods such as RT- PCR compared to microscopic methods has a higher sensitivity and specificity however, it is not widely used due to its expensive equipment and the time requested. The use of nucleic acid sequence based amplification (NASBA) method is highly valuable for diagnosis of live parasite because there is no need for to use Thermo cycler. We aimed to assess sensitivity and specificity of NASBA for molecular detection of cutaneous Leishmaniasis.
Material and Methods: First, the RNA was extracted from 28 skin biopsies suspected cutaneous Leishmaniasis. Then, by means of specific primers designed for 18srRNA region, this region was amplified using NASBA isothemal amplification. To increase the sensitivity, the product was electroforesed in TBE (IX) buffer, using Syber Gold Flourecent probes. Using specific primers, RT- PCR was conducted on the samples too.
Result: For diagnosis of Leishmania parasites, NASBA and RT-PCR had the sensitivity of 81% and 51%, respectively, and specificity of 100%.
Conclusion: NASBA isothermal method with high sensitivity and specificity can be applied for identification of cutaneous leishmaniasis.
Keywords: Cutaneous Leishmanisis, NASBA, 18S rRNA
Masoud Soosaraei, Ahmad Daryani, Shahabeddin Sarvi, Mohamad Taghi Rahimi, Mahdi Fakhar, Hajar Ziaei Hezarjaribi, Mehdi Sharif,
Volume 14, Issue 5 (Sep-Oct 2020)
Abstract
Background and objectives: Intestinal parasitic infections are a major public health problem worldwide, especially in developing countries. It is estimated that around 3.5 billion people are infected with intestinal parasites. Human intestinal parasites (HIP) are clinically important due to broad epidemiological distribution, reinfection and drug resistance. In the last decades, bioactive compounds from herbs were used against a wide variety of microorganisms including parasites. We aimed to perform a systematic review on studies on the effects of medicinal herbs on HIPs in Iran.
Methods: Relevant scientific publications until April, 2015 were extracted from five English databases (PubMed, Google Scholar, Ebsco, Science Direct and Scopus) and four Persian databases (Magiran, Irandoc, IranMedex and the Scientific Information Database).
Results: A total of 18 papers and two
dissertations met the inclusion criteria. Overall, 22 different plant extracts were used against
Giardia lamblia,
Entamoeba histolytica,
Cryptosporidium and
Hymenolepis nana. Based on the results, the extracts could exert time- and dose-dependent inhibitory effects against the tested HIPs. Five plants types including
Allium,
Chenopodium botrys, Carum copticum, F. asafoetida and
Artemisia annua were able to completely inhibit the tested parasites, while
Thymus vulgaris and
A. paradoxum showed the lowest inhibitory effect (7%).
Conclusions: Given the findings, it is recommended to conduct in vivo studies on medicinal herbs with favorable in vitro effects against HIPs.
Masoud Soosaraei, Ahmad Daryani, Mehdi Sharif, Shabeddin Sarvi, Hajar Ziaei Hezarjaribi, Mahdi Fakhar,
Volume 15, Issue 1 (Jan-Feb 2021)
Abstract
Background and objectives: Cryptosporidium spp. is a major cause of gastrointestinal illness in humans. There are no data available on geospatial distribution of Cryptosporidium spp. in the Mazandaran Province, Iran. Therefore, the aim of this study was to determine the spatial patterns and demographic factors associated with Cryptosporidium spp. infection in the Mazandaran Province, North of Iran.
Methods: Fecal specimens were collected from diarrheic individuals (n=215) who were referred to health centers in the Mazandaran Province during 2014-2015. The specimens were examined for presence of Cryptosporidium spp. oocysts by Ziehl-Neelsen acid-fast staining.
Results: Cities of Sari, Neka, Noshahr and Behshahr were identified as disease hotspots. The prevalence of Cryptosporidium infection was significantly higher in subjects under 10 years of age as well as those living in low-altitude areas and rural areas without access to standard water sources.
Conclusion: Our findings and the GIS-derived data could be used to facilitate cryptosporidiosis surveillance and monitoring of Cryptosporidium spp. distribution in the study area.
