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Showing 6 results for Zali

Tajeddin, E. (msc), Jahani Sherafat, S. (msc), Seyyed Majidi, M. R. (md), Alebouyeh, M. (phd), Nazemalhosseini Mojarad, E. (msc), Pourhossengholi, A. (phd), Mohammad Alizadeh A H (md), Zali, Mr (md),
Volume 5, Issue 2 (Autumn – Winter 2011[PERSIAN] 2011)

Abstract Background and objectives: Bile in healthy people is a sterile fluid and presence of any microorganism can be a marker for a disorder like cholelithiasis. The aim of this study was to determine the frequency of bacterial agents in the bile of patients with bilestone, malignant pancreatic and biliary diseases. Material and Methods: One hundred and two bile samples were obtained, during six months in 2011, from patients subjected to ERCP in Taleghani hospital, Tehran. First, Patientchr('39')s clinical data, the type stone, and their disease status were studied, and then the microbiological investigations, such as culture, identification of the bacteria and detection of their counts, drug susceptibility testing and molecular tests (16s rDNA PCR) performed on all the samples. Higher than 103 bacteria counts for each sample, in the absence of underlying infections, was considered as stable colonization. We run SPSS version 13 to analyze the data. Results: Out of 42(41.1%) positive bile culture samples, 59 bacterial isolates are detected by conventional methods. Of culture negative samples, seven have bacterial DNA indicated by PCR method. The most isolated bacteria are E. coli (%34.4), Enterococcus spp. (%19.7), Klebsiella pneumoniae (%18) and Pseudomonas aeruginos (18%). The most frequent stones are cholesterol, black pigment and brown pigment, respectively. There is no significant association between the diseases, stones and types of bacteria. Previous antibiotic usage (44.6%) is meaningfully more than that of other biliary problems (p=0.01) Conclusion: The presence of bacteria, Escherchi coli and Entrococcus which are the most in bile samples, is considered as a risk factor in pathogenesis of biliary disorders. Further studies on the pathogenesis and pathophysiological effects of bacteria can help us to clarify the role of bacteria in producing bile stones. Key words: Bile stones, Bacteria, ERCP, Antibiotics.
P Torabi, M Azimirad, Z Hasani, M Janmaleki, H Peirovi, M Alebouyeh, Mr Zali,
Volume 8, Issue 1 (spring[PERSIAN] 2014)

Abstract Background and Objective: This study was aimed to determine the extent of bacterial contamination and drug resistance patterns of isolates colonized in colonoscope and endoscope and in relevant personnel. Material and Methods: A total of 107 samples were obtained from staff of endoscopy and colonoscopy units (SEU and SCU) and gastroenterological imaging equipment. For isolation and identification of the bacteria, swab culture method and biochemical identification test were used, respectively. Antimicrobial resistance profiles, multi-drug resistance (MDR) patterns and phenetic relatedness of these isolates were also analyzed according to standard methods. Results: Most frequent pathogenic bacteria among the SEU and gastroenterological imaging related equipments were included S. aureus (20.8 % and 0 %) Enterococcus spp. (0 % and 5.4%) Pseudomonas spp. (0% and 13.5 %), and Clostridium difficile (0% and 12.5%). Analysis of resistance phenotypes showed a high frequency of MDR phenotypes among the SEU (82.1%), and also in endoscopes, colonoscopes, and other equipments (20%, 50% and 100%, respectively). Phylotyping of S. epidermidis isolates showed the role of staff in transmission of resistance strains to medical equipments and also circulation of strains with identical resistance phenotype among the studied samples. Conclusion: High frequency of pathogenic bacteria in colonoscopes, endoscopes and in the staff of endoscopy & colonoscopy units, and also contamination of these instruments with MDR pathogens emphasize the need for proper disinfection of endoscopes and colonoscopes and also instruction of staff in these units. Key words: Bacterial Contamination Endoscope Colonoscope Antimicrobial Resistance Gastrointestinal Disease.
Shokoohi Zade, L, Mohabbati Mobarez, A, Alebooye, M, Ranjbar, R, Zali, Mr,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)

Abstract Background and Objective: some of predisposing factors for enterococci colonization are hospitalization in ICU, prolonged use of antibiotics and continued bed rest in hospital. In this study antibiotic resistance of enterococcus in hospitalized patients of four hospitals in Tehran were studied. Material and Methods: the Clinical samples were taken from patients admitted to the ICU, from September 2011 to April 2012. Enterococci isolates were confirmed by biochemical tests, and Enterococcus faecalis and Enterococcus species by species-specific ddl genes. The disk diffusion and micro agar dilution susceptibility tests were performed according to Clinical and Laboratory Standards Institute (CLSI). Results: of 41 isolates in ICUs, 22 (5.52%) were E. faecium and 19 (5.47%) were E. faecalis. Most of E. faecium was isolated from urine and E. faecalis from trachea specimens. The rate of resistance to vancomycin, ampicillin, gentamicin, chloramphenicol and nitrofurantoin in E. faecium isolates was more than that of E. faecalis and the rate of resistance to tetracycline, ciprofloxacin and erythromycin was the same in both of them. MIC50 in vancomycin and ampicillin resistant E. faecium isolates was greater than 256 microgram and the MIC50 in gentamicin resistant isolates was more than 1024 microgram. . Conclusion: The presence of multi-resistant E. faecium strains in ICUs can be a serious warning for physicians and patients. Key words: Enterococcus faecium, Enterococcus faecalis, ICU, Antibiotic Resistance

Hamid Asadzadeh Aghdaei , Zahra Pezeshkian, Meghdad Abdollahpour-Alitappeh , Ehsan Nazemalhosseini Mojarad , Mohammad Reza Zali,
Volume 12, Issue 4 (Jul-Aug 2018)

          Colorectal cancer remains a leading cause of morbidity and mortality worldwide. Most colorectal cancers begin as a growth of tissue called a polyp, some types of which can change into cancer over the course of several years. Angiogenesis, the formation of new blood vessels, is known to play a key role in the initiation, growth and progression of polyp and colorectal cancer. In this review, we discuss the mechanisms involved in colorectal cancer and polyp angiogenesis.
          Keywords: Colorectal Neoplasms, Polyps, Angiogenesis
Somaieh Sabzali, Majid Bouzari,
Volume 14, Issue 4 (Jul-Aug 2020)

   Background and objectives: are divided into two species: Salmonella enterica and Salmonella Salmonella bongori. S. enterica has more than 2,500 serotypes. Serovars of S. enterica such as Typhimurium, Enteritidis, Paratyphi B, Paratyphi A and Newport are associated with human infections. Approximately 75% of human Salmonella infections have been associated with contaminated food such as eggs, chicken, beef, pork, dairy products, fruits and vegetables. The aim of this study was to determine the frequency of Salmonella strains isolated from various food sources in Isfahan, Iran.
    Methods: Forty Salmonella strains were isolated from 450 suspected cases referred to the veterinary reference laboratory of Isfahan Province. The isolates were identified by differential and serotyping tests and then confirmed by PCR. A phylogenic tree was constructed with 34 sequences by neighbor-joining method using the MEGA7 software (version 7.1). 
    Results: Overall, 10 Salmonella serovars were isolated from 32 chicken meat, three beef and five egg shell samples. S. enterica serovar Ouakum (20%), S. Enteritidis (17.5%) and S. Typhimurium (17.5%) were the most common serovars, while S. enterica serovar Nitra (2.5%) was found as the least prevalent isolate.
    Conclusion: In this study, S. Typhimurium species is placed in different clusters along with sequences reported from different parts of the world, indicating that the serovars are circulating all over the world.

Mohammad Arefi, Abbas Abdollahi, Ayyoob Khosravi, Abdolavahab Moradi, Seyed Hamid Aghaee-Bakhtiari, Naimeh Javid, Mehdi Evazalipour, Anvarsadat Kianmehr,
Volume 15, Issue 2 (Mar-Apr 2021)

Background and objectives: Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality in the world. MicroRNAs (miRNAs) have potential as diagnostic biomarkers for various diseases including cancer. This study was undertaken to investigate expression of miR-21 before and after surgery in patients with hereditary CRC.
Methods: After collecting blood samples from 39 patients and 39 healthy controls, total RNA was extracted by the TRIzol method. Following cDNA synthesis, expression of miR-21 in serum of subjects was evaluated using real-time PCR, along with two reference genes, let-7d and let-7g. The real-time expression results and Ct values were collected and analyzed based on the 2-∆∆ct method.
Results: In spite of tumor removal, serum miR-21 expression levels was significantly higher in hereditary CRC patients compared with controls (P=0.022).
Conclusion: Our results confirmed that samples from hereditary cases of CRC must not be included in experiments on the diagnostic potential of miRNAs.

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