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Mahmoud Alebouyeh , Zahra Abedi , Hossein Rastegar , Hasan Bagheri , Javad Vaez, Behrouz Akbari-Adergani ,
Volume 9, Issue 5 (Nov,Dec-2015 2015)
Abstract

Abstract

       Background and Objective: Aluminum salts are among the most common useful additive compounds in preparation of human and animal vaccines. Aluminum phosphate and aluminum hydroxide are two additives that show good immunoadjuvant effects with many antigens. Aluminum-containing vaccines lead to a better and longer immune response compared to adjuvant-lacking vaccines. The Chromogenic methods used for determination of aluminum amounts in manufacturing centers are  time-consuming and requires some experienced technicians to obtain accurate results. This study aimed to design and validate a simple polarographic method to measure aluminum in recombinant hepatitis B vaccine.

       Methods: In this study, the effects of temperature, pH, potential range and potential scan rate on the polarographic method of measuring aluminum in hepatitis B vaccine was evaluated and  the optimal values for each of these factors were achieved.

       Results: In order to measure aluminum, temperature of 60 °C and pH of 4.5 were found as the optimal values. Implementation of polarographic method in the potential range of -0.25 to 0.1 volts had a better signal.

       Conclusion: Since the polarography method is more simple, accurate and faster than the chromogenic methods, it is suitable to be used for the measurement of aluminum in hepatitis B vaccine and it is recommended to be used in quality control laboratories for biological products.

         Keywords: Adjuvant, Hepatitis B Vaccine, Polarography, Aluminum.


Hamid Vaez , Vahid Vaez , Farzad Khademi ,
Volume 11, Issue 6 (Nov - Dec 2017)
Abstract

ABSTRACT
           Background and Objectives: Pseudomonas aeruginosa is an important non-fermenting gram-negative hospital-acquired pathogen. Treatment of P. aeruginosa infections has become more challenging due to overexpression of efflux pumps. The aim of the present study was to apply in silico analysis to evaluate the structure of the efflux pump regulatory protein, MexR, and impact of mutation on its stability and function.
         Methods: Different bioinformatics tools including EXPASY, PROTEER, TECCOFFE, iStable, I-Mutant 2, STRING, ESPript, GOR IV, and PDB were used in the study.
          Results: Aliphatic and instability indices were 104.15, and 46.52, respectively, indicating that the protein has a relatively short half-life. Most mutations decreased protein stability. Twenty-four mutations were identified as deleterious, with negative impact on the protein’s function.
         Conclusion: Determination of structure, variability, and function of MexR could be useful for modeling of treatment and control of multidrug resistant P. aeruginosa, with overexpressed efflux pump. We found that MexR is a relatively unstable and conserved protein and the majority of mutations decrease its stability.

         Keywords: Pseudomonas aeruginosa, MexR protein, Drug resistance, drug resistance multiple.



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