Showing 2 results for Soltan Dallal, MM
Ghanadan, M, Akbari, N, Soltan Dallal, Mm,
Volume 8, Issue 5 (winter[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Cream, a rich dairy product, with a neutral PH and low preservation time is a suitable medium for microbial growth. Salmonella is one of the most important pathogens in causing food poisoning and human gastroenteritis. This study aimed at investigating the quality of traditional cream for the bacterial contamination. Material and Methods: In this cross-sectional study, 196 non-pasteurized cream samples were collected from 5 regions of Hamedan, Iran. After dilution in phosphate buffer and serial dilution preparation, Salmonella was transferred to Rappaport-Vassiliadis (RV) enrichment medium according to CDC guidelines. After 24 h incubation at 37 ° C, a loop was inoculated in MacConkey and Hektoen Enteric (HE) Agar. The suspected colony phenotype was examined and their identification confirmed by API-20 E. Results: The samples (29%) were contaminated with at least one kind of bacteria, Salmonella Spp (4.59%) and Yersinia Spp (2.55%). The other bacteria like Escherichia, Enterobacter, Klebsiella, and Citrobacter were also isolated. Nine samples were contaminated with two kinds of bacteria. Conclusion: The presence of bacteria such as Salmonella and Yersinia in unpasteurized cream indicates that more quality control needs to be applied to the traditional crème produced in the city by health control office of food products. Keywords: Cream, Salmonella SPP, Coliform, Yersinia Enterocolitica, Hamedan
Soltan Dallal, Mm, Rahbar, M, Douraghi, M, Rahimi Forooshani, A, Khan Babaei, Gt, Mobarhan, M, Ghasemi, F,
Volume 8, Issue 5 (winter[PERSIAN] 2015)
Abstract
Abstract
Background and Objective: Cystic fibrosis (CF) is an autosomal recessive genetic disease and Pseudomonas aeruginosa is one of the most common bacteria colonized in CF patients. Growing resistance of this bacterium to antibiotics now a day is a challenge of controlling infection in CF patient. In this study colonization of CF patients with Pseudomonas aeruginosa and antibiotic susceptibility pattern of isolated strains were examined.
Material and Methods: From 100 CF patients, during a year, sputum and bronchial swabs were collected. After culturing the samples, some of them were reported as Pseudomonas aeruginosa using biochemical tests. Mucoid strains of Pseudomonas aeruginosa were identified the same as non-producing alginate strains while for catching single pure colony, repeated passage was used. For determining antibiotic resistance of Pseudomonas aeruginosa to some antimicrobial agents Kirby-Bauer method based on CLSI was used.
Results: Of 100 samples, 40 (40%) were positive for Pseudompnas aeruginosa. The prevalence of P. aeruginosa was 23.8, 36.84 and 80% at the age of 1-3, 4-12 and 13, respectively.
Conclusion: Statistically, there is a significant difference between age and contracting with Pseudomonas aeruginosa in that the higher the age the more colonization with Pseudomonas aeruginosa.
Key words: Pseudomonas Aeruginosa, Cystic Fibrosis, Drug Resistance
