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Showing 7 results for Salehi

Z Shahandeh, F Sedighian, Mt Salehi Omran, F Saberian,
Volume 3, Issue 1 (Spring - Summer 2009[PERSIAN] 2009)

Abstract Background and objectives: Coronary Artery Disease is one of the most important causes of death in the world. Atherosclerosis is a complex disorder and many factors cause it . In recent years , a relationship between infectious agents, particularly Chlamydia pneumonia, and atherosclerosis was found. This research was aimed at serological studying of Chlamydia pneumonia in the coronary artery patients and healthy ones. Material and Methods: We carried out this cross-Sectional study on 102 patients with CAD and 142 individuals without any history of CAD. The subjects were admitted to Dr. Beheshty hospital, Babol, from July 2005 to September 2006. The subjects’ serums were collected to detect specific anti Chlamydia pneumonia antibodies (IgA and IgG), using ELISA method. Data were analyzed by chi-square, using SPSS software. Results: The mean age of patients and healthy subjects were 60.6 and 43.7 years, respectively. Fifty-nine point eight percent of cases and 40.8% of healthy subjects are men. The results show that 45.1% of patients and 47.9% of healthy subjects have positive titer of IgG and negative titer of IgA (chronic). There is no significant correlation between patient and healthy groups (P=O.698). Also 88.2% of patients and 81% of healthy group have positive titer of anti Chlamydia pneumonia antibody (IgG). Conclusion: In spite of high titer of IgG, there is no significant relationship between Chlamydia pneumonia and atherosclerosis. It seems that further experiments are necessary to prove the relationship between Chlamydia and atherosclerosis. Key words: Atherosclerosis, Chlamydia pneumonia, IgA, IgG
Nazari, H, Habibeh Najar, H, Emadi, A, Abasi, Y, Salehiyan, A, Monem, M, Ghods, F, Mohammadi, A., Khaleghian, A,
Volume 5, Issue 2 (Autumn – Winter 2011[PERSIAN] 2011)

Abstract Background and objectives: Glucose-6-phosphate dehydrogenase (G6PD) is an enzyme in the pentose phosphate pathway. G6PD deficiency (an X-linked recessive hereditary disease) is an inherited condition affecting approximately 3% of the people globally. This deficiency can cause hemolytic anemia and jaundice in neonates. The goal of this study is to detect the prevalence of G6PD deficiency in neonates referred to Semnan province screening lab Material and Methods: This cross sectional study, from 2007 to 2010, was conducted on the basis of country planned program and in line with neonatal screening tests. Blood samples were taken from the heels of 3-5 day neonates. Assessment of G6PD was done by rapid fluorescent spot test. The Prevalence of G6PD deficiency in boys and girls was compared by chi square (p<0.05(test. Results: Of 9353 newborns referred to semnan province screening Lab., 4820(51.53%) are males and 4533(48.47%) females. Three hundred (3.2%) of them suffer from G6PD deficiency. Of these, 263 (5.45%) are males and 37 (0.81%) females. The ratio of male to female is 7 to 1. Conclusion: The prevalence of G6PD deficiency is detected 3.2% in Semnan province and its frequency in boys is more than that of girls, which is expected. Key words: Glucose -6-phosphate dehydrogenase, Neonatal screening, Fauvism
Amoozgari, Z, Salehi Pour Bavarsad, S, Noorbehbahani, M,
Volume 9, Issue 1 (March, April[PERSIAN] 2015)

Abstract Background and Objective: Snake venom is a complex of several toxic elements and enzymes. It has the agents with the ability to destroy cellular and subcellular membrane and to bring about hemolysis of red blood cells (RBC). Two types of direct and indirect hemolytic activity are known in snake venom in that phospholipase A2 is responsible for the indirect lysis. The aim of this study was to investigate the effect of α-lipoic acid on hemolytic activity of Iranian Vipera Lebetina venom. Material and Methods: Protein concentration of the crude venom of Vipera Lebetina was determined using bovine serum albumin as a standard. Direct hemolytic activity of venom was determined by using the Human RBC and Indirect hemolytic activity was assayed on RBC in the presence of egg yolk. Then, α-lipoic acid with different concentrations in 100 mM Tris-HCL buffer was applied and its effect on hemolysis of RBC was studied. Results: direct hemolytic activity on RBC was not observed while its indirect activity was detected to be increased proportional to different concentration of α-lipoic acid. The range of indirect hemolysis was increased up to 60% by 60µm α-lipoic acid. Conclusion: Not only has α-lipoic acid no inhibitory effects on the hemolytic activity of Iranian Vipera Lebetina venom but also has the positive effects on it. Keywords: Iranian Vipera Lebetina Venom, Direct Hemolytic, Indirect Hemolytic, α-Lipoic Acid, Phospholipase A2
Ania Ahani Azari , Taghi Zahraei Salehi , Bahar Nayeri Fasaei ,
Volume 10, Issue 6 (Nov-Dec-2016 2016)


        Background and Objective: In this study, we compared the susceptibility of Salmonella typhimurium phoP mutant and its parent to stress conditions that the pathogen may encounter in a host.

        Methods: For this purpose, we used the phoP deletion mutant constructed in our previous study. In order to test the in vitro susceptibility of the mutant to stress factors, the effect of acidic pH, heat, bile salts and polymyxin on growth of the mutant was examined. Then, minimum inhibitory concentration and minimum bactericidal concentration of bile salts and polymyxin were determined. Salmonella typhimurium 14028 was used as the parent strain.

         Results: The mutant was highly susceptible to bile salts and polymyxin in comparison with the parent strain, but no difference was observed in their susceptibility to acid and heat.

          Conclusion: This study confirms the role of the phoP in resistance of Salmonella to polymyxin and bile salts. Mutation in the phoP leads to susceptibility of the mutant to bile and cationic antimicrobial peptides.

         Keywords: In vitro, Mutant, Salmonella typhimurium, Disease Susceptibility.

Zahra Rahimi , Mansour Salehi , Abbas Dousti ,
Volume 11, Issue 3 (May-Jun 2017)

         Background and objective: Approximately 50 million people worldwide (1% of the worldchr('39')s population) suffer from epilepsy. Among 700 thousand people with epilepsy in Iran, 20% have refractory epilepsy. Accumulation of leukocytes in patientschr('39') brain parenchyma is thought to be related to different types of epilepsy. Recent clinical observations suggest that therapeutic strategies that interfere with leukocytes or cause them to migrate may have therapeutic efficacy in epilepsy. The aim of this study was to identify treatment-resistant patients, and investigate the association between polymorphism rs1024611 in CCL2 gene and drug resistance in patients with epilepsy in Isfahan, Iran.
        Methods: Blood samples were taken from 50 patients with intractable epilepsy (case group) and 50 drug-responsive patients with epilepsy (control group). Genomic DNA was extracted from peripheral blood by salting out method. Specific primers were designed by Oligo 7 software to investigate polymorphism rs1024611 using PCR-RFLP. The preliminary results for a number of samples were confirmed by sequencing.
        Results: The results of this study showed that there was a significant relationship between intractable epilepsy and presence of C allele.
        Conclusion: Similar to previous study, we found a significant association between CCL2 gene polymorphism and drug-resistant epilepsy.
        Keywords: Epilepsy, Drug Resistance, Polymorphism, CCL2.
Ali Salehi, Hajar Abaszadeh, Parvin Farzanegi,
Volume 13, Issue 4 (Jul-Aug 2019)

Background and objectives: Pyruvate dehydrogenase kinase 4 (PDK4) is an enzyme involved in the regulation of glucose and fatty acid metabolism and homeostasis. The purpose of this study was to investigate effects of continuous exercise and resveratrol supplementation on insulin resistance and expression of PDK4 in the soleus muscle of elderly rats with type 2 diabetes.
Methods: After inducing diabetes in 42 old male rats (mean weigh: 250-300 g, aged about two years), the rats were randomly divided into six groups: healthy control, diabetic control, continuous exercise, supplementation, continuous exercise + supplementation and sham. The continuous exercise group was subjected to eight weeks of running, five sessions a week. Daily 20 mg/Kg body weight resveratrol supplementation was administered to the supplementation and supplementation + exercise groups. To evaluate insulin resistance, the HOMA-IR insulin resistance index was calculated as the product of insulin (μIU/mL) and glucose (mmol/L) divided by 22.5. Expression of the PDK4 gene in the soleus muscle was analyzed by RT-PCR. Data were analyzed with IBM SPSS Statistics 23 using one-way ANOVA and Tukeychr('39')s post hoc test at a significance level of 0.05.
Results: Resveratrol alone or combined with continuous exercise significantly increased PDK4 expression and slightly decreased insulin resistance in the subjects.
Conclusion: Our results show that diabetes is associated with reduced PDK4 expression and increased insulin resistance. These effects can be effectively reversed in the soleus muscle of elderly diabetic rats by resveratrol supplementation alone or combined with continuous exercise.
Mina Eghbali, Majid Baserisalehi, Masood Ghane,
Volume 14, Issue 3 (May-Jun 2020)

Background and Objectives: Moraxella catarrhalis is considered as an emerging pathogen and a new nosocomial infection agent. This study was conducted to isolate and identify M. catarrhalis from clinical samples (respiratory tracts) and assess them for antimicrobial susceptibility patterns.
      Methods: In total, 280 samples were collected from patients with respiratory tract infection, and 120 samples were obtained from healthy individuals in the control group. The isolates were identified by phenotyping and genotyping methods, and their antibiotic susceptibility was  evaluated using disk diffusion methods. The presence of β-lactamase and efflux pump activity were specified via phenotypic methods. Finally, Bro and acrA genes in the isolates were detected by PCR technique.
      Results: The frequency of this bacterium was 9.64% (27 out of 280) in patients with respiratory tract infection and 4.16% (5 out of 120) in the control group. Although the isolates were resistant to penicillin, they had various responses against other antibiotics. The results obtained from molecular method showed that 90.6% and 84.3% of the isolates possessed Bro and acrA genes, respectively. There was a significant relationship (P<0.05) between the presence of Bro and acrA genes and antibacterial resistance to ampicillin, amoxicillin, cefazolin, cefuroxime, and chloramphenicol.
      Conclusion: Our findings confirmed the existence of M. catarrhalis in patients with respiratory diseases and the high prevalence of antibiotic resistant genes in M. catarrhalis isolates. Therefore, timely diagnosis and successful treatment can play important roles in preventing their spread.

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