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Showing 9 results for Rostami

M Rostami, M Jarfi,
Volume 3, Issue 2 (Autumn – Winter 2010[PERSIAN] 2009)

Abstract Background and objectives: Smokers are exposed to significant quantities of ROS (Reactive Oxygen Species) and The Level of Nitric Oxide (NO), the primary vasodilator produced by endothelial cells, is changed by cigarette smoking. Cigarette smoking is associated with impaired endothelium-dependent vasodilatation and cardiovascular disease (CVD). The aim of this study is to determine the level of serum nitrite, nitrate and malondialdehyde (MDA) levels in smokers. Material and Methods: In this descriptive analytical study, 60 healthy male smokers and 60 male non-smokers (control group) were selected by a Purposive sampling and then serum levels of nitrite, nitrate and MDA in all patients were determined and compared to together. Results: Serum nitrite and nitrate level in smokers are 10.4±3.1 and 19.6±5.9 and in non-smokers is 14.6±4.4 and 29.3±6.7 (p<0.00001) μmol/L, respectively. The results show that smokers’ are significantly lower than non-smokers’. Serum MDA level in smokers (11.7±2.6 μmol/L) is significantly higher (p<0.00001) than non-smokers (8.3±1.9 μmol/L). Conclusion: Based on the results, serum level of nitrite and nitrate are lower and MDA is higher in smokers. This difference can be related to CVD in smokers. Keywords: Smokers, Non-smokers, Nitrite, Nitrate, Malodialdehyde (MDA), cardiovascular disease (CVD).
M Kadkhodaei Elyaderani, M Rostami, B Keikhaie, M Pedram,
Volume 4, Issue 1 (Spring - Summer 2010[PERSIAN] 2010)

73/ Spring summer 2010, Vol.4, No. 1 Medical Laboratory Journal Correlation between Nitric oxide (NO) & Asymmetric dimethylargininie (ADMA) Hemoglobin Concentration in sickle cell patients Abstract Background and objectives: The importance of Nitric oxide (NO) and Asymmetric dimethylargininie (ADMA) in pathophysiology of Sickle cell disease (SCD) is being increasingly clarified. Since very few of the studies have been conducted in the word and no study has been carried out in Iran, especially in Khuzestan province where is the main center of Sickle Cell disorder (SCD) in Iran, We decided to conduct the present study. Material and Methods: EDTA anticoagulated plasma samples were obtained from 35 healthy controls (Hb AA), 35 heterozygous (HB AS) and 35 homozygous (HB SS) sickle cell anemia patients. Plasma concentration of NO was measured by Colorimetric and Griess reaction and the concentration of ADMA by employing ELISA method. Then the results were analyzed by tstudent test and OneWay ANOVA. Results: There is a positive significance correlation between Hemoglobin (Hb) and NO in SS (r=0.703) and AS (r=0.366) groups. Also, a negative correlation between Hb and ADMA in SS (r=-0.786) and AS (r=-0.478) groups is seen. No correlation is found between these parameters in AA group. Conclusion: The prevention of Hb concentration decrease and prescription of NO donors and (or) ADMA disintegrators can be helpful for improving clinical signs of sickle cell patients. Key words: Nitric oxide (NO), Asymmetric dimethylargininie (ADMA), Sickle cell disease (SCD). Kadkhodaei ElyaderaniM (PhD) Associateprof. of Biochemistry, Dept of Clinical Biochemistry and Research Center of Thalassemia & Hemoglobinopathy, School of Medicine , Jundishapur University of Medical Sciences, Ahwaz , Iran Rostami M (MSc) MSc of Biochemistry, Dept of Clinical Biochemistry, School of Medicine , Jundishapur University of Medical Sciences, Ahwaz , Iran . Keikhaie B (PhD) Assistant Professor of Pediatric Hematology and Oncology, Shafa Hospital and Research Center of Thalassemia& Hemoglobinopathy, Jundishapur University of Medical Sciences, Ahwaz, Iran. PedramM (PhD) Professor of Pediatric Hematology and Oncology, Shafa Hospital and Research Center of Thalassemia & emoglobinopathy, Jundishapur University of Medical Sciences, Ahwaz, Iran. Corresponding: Kadkhodaei ElyaderaniM Email:
M Rostami, F Tohidi, M Sharbatkhori, H Taherkhani, A Eteraf, R Mohammadi, F Maghsoodloorad,
Volume 6, Issue 2 (Autumn- Winter [PERSIAN] 2012)

Abstract Background&Objectives: High prevalence of parasitic infections can be the result of the subclinical infections. Some regular and ongoing epidemiological studies are needed to combat and control these infections. This study was conducted on elementary school students to achieve an overview of the spread of parasitic infections in Gorgan. Material & Methods: In this cross-sectional study, using random cluster sampling, 800 of 7-12 year-old students were selected from 18 primary schools in Gorgan. The stool samples taken on three consecutive days were examined by two methods including direct smear and formalin-ether concentration. Results: Overall, 230 (28.8%) are infected by intestinal parasites. The protozoa are Blastocystis hominis (122 15/2%), Entamoeba coli (93 11/6%), Giardia intestinalis (79 9.9%), and Entamoeba histolytic (8 1%). The Helminthes are Hymenolepis nana (12 1.5%), Entrobius vermicularis (10 1.2%), Ascaris lumbricoides in 4 (0.5%) and hook worms (3 0.4). Conclusion: Based on the results, the prevalence of intestinal parasites is quite high among primary school students especially in families with low education level, income and sanitary level. Hence, it is a necessity to promote the hygienic knowledge of the students . Key words: Prevalence, Intestinal parasites, Primary schools students, Gorgan
M Alimohammadi, M Rostami, M Jorfi,
Volume 6, Issue 2 (Autumn- Winter [PERSIAN] 2012)

Gestational diabetes mellitus (GDM) is the most common metabolic disorder during pregnancy which is associated with 3 to 4 folds of increase in the risk of maternal and fetal morbidity, if it is not diagnosed early (1). Gestational diabetes occurs from 1% to 14%, and 90% of pregnancy-related diabetes has been related to gestational diabetes (2). Glucose challenge test (GCT) and glucose tolerance test (GTT) are recommended by American Diabetes Association (ADA) for screening and diagnosing of gestational diabetes (3). In this cross-sectional study, the subjects were the pregnant women during 24-28 weeks of gestation referred to Khatam-o-allanbia laboratory of Arak city in 2009. First, we explained the aim of the project and then obtained the written consent. After being fast for at least 8-12 hours, five ml of their venous blood was drown. For GCT, the blood samples were obtained one hour after consumption of 50g of glucose powder. The Individuals whose GCT was equal or higher than 130 mg/dl were introduced for GTT in the future days. After 8-12 hours of being fast, blood samples were taken to perform GTT. Then after eating 100 grams of glucose powder, their 1, 2 and 3 hour -venous blood samples were drown to perform glucose test, using glucose testing kit of Pars Azmun Company and Mindary BS-300 autoanalyser. The average age of referred pregnant women is 27.37±10.24 year. Of 417, 403 (96.64%) have normal fasting plasma glucose (≤ 99 mg/dl) and 14 (3.36%) have abnormal fasting plasma glucose (≥ 100 mg/dl). When normal GCT is considered ≥140 mg/dl and ≥130 mg/dl, 357 (85.61%) and 308 (73.56%) have normal GCT and 60 (14.39%) and 109 (26.14%) have abnormal GCT, respectively. The Average of fasting plasma glucose (FPG) and GCT are 83.86 (73-243) and 118.32 (140-351) mg/dl, respectively. In 9 (64.29%) of women with abnormal FPG, the GCT test is also abnormal. By performing GTT for the women whose GCT was equal or higher than 130 mg/dl, 24 (5.76%) were identified with gestational diabetes and introduced for treatment. If 140 mg/dl was considered as a cutoff point of GCT, four women (0.94%) would falsely be non-diabetic. Based on this study, the prevalence of GDM is 5.74% that is not so high, compared to the range of 4.8 to 7.4% of the country (4). A study conducted by Soheilykhah et al. on 1071 pregnant women with GCT of ≥ 130 mg /dl, 31.9% of the women had abnormal GCT. After doing GTT, 110 patients (10.2%) have been diagnosed with gestational diabetes (2) that is higher than the prevalence of other studies in Iran. Due to results of this study and other studies, it seems that the GCT is not a reliable test for screening gestational diabetes. On the other hand, if GCT is going to be used, further studies are required to review the current Cutoff point of this test. Key words: Glucose challenge test (GCT), Fasting plasma glucose (FPG), Gestational diabetes.
A Choupani, Z Rostami, Aa, A Abdullahi,
Volume 7, Issue 1 (spring[PERSIAN] 2013)

Abstract Background and Objective: Helicobacterpylorus is the major cause ofinflammation andulcer instomach, and immunoglobulin IgG is one of the antibodies produced against it, which is important in the course and diagnosis of the previoussufferers. The awareness of the prevalence of this disease can be helpful for the physicians to choose the way of treatment. Material and Methods: In these cross-sectional study, 516patients referred to laboratory was studied. After separating the serum, Antibody Helicobacter pylori IgGtest wasdone by ELISA method. Results: of 516, 156 (30.2 %) of the patients have a positive result, 51(32.7%) are males and 105 (67.3 %) are females. Positive percent of males (43.5%) is greater than females (26.5%).Over-45-year-old women (9.8 %) have the highest percentage of disease titers. Conclusion: The percent of positive cases in men is more than that the women. Over-45-year-old women (9.8 %) have the highest percentage of positive case. Keywords: Helicobacter pylori IgG, Tehran, ELISA
F Niknazar, M Aberumand, R Rostami, M Seyed Tabib,
Volume 7, Issue 1 (spring[PERSIAN] 2013)

Abstract Background and Objective: Some studies show that, there is a relationship between decrease of serum magnesium (Mg) level and the insulin resistance in diabetic type II patients. The aim of this study was to compare the Mg level in in type II diabetic with non-diabetic individuals. Materials and Methods: Diabetic patients(n=35) and control group (n=35),<35 years old, were randomly selected from the hospital of Imam Ali, Andimeshk. To compare diabetic patients and control group, the t-student statistical method and SPSS software, were used. Results: Mg levels in diabetic and non diabetic groups were 1.33±0.27 and 1.62±0.21 mg/dl, respectively, and the difference was statistically significant (p=0.0000014). A reversed significant relationship was seen between FPG and Mg among type II diabetic patients, whereas no significant relationship was seen between Mg and other variables, (TG, Chol and HDL). In non-diabetics group, there was a reversed significant relationship between the Mg and Chol, Whereas, no relationship was seen between other variables and magnesium. Conclusion: In diabetic patients, with increased level of FPG, serum Mg levels were decreased significantly. Moreover, serum Mg levels in diabetic patients were significantly lower than non-diabetics. Keywords: Magnesium, Diabetes type 2, Cholesterol, Triglyceride, High density lipoprotein
Ebrahimzad, Mousavi Moh, Jamshidi Ali, Mohammadzadeh Rostami F (msc),
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)


Background and Objective: Oral candidiasis is a common opportunistic infection in diabetic patients. Candida albicans is colonized on different oral surfaces such as tongue, palate, dental caries and plaques.  This study aimed to determine the prevalence of   Candida albicans in the mouth of   diabetic patient.

Material and Methods: This cross - sectional study was conducted on 120 diabetic patients. After completing the questionnaire, the   saliva samples were obtained and cultured   on sabouraud agar medium containing 0.005% chloramphenicol. For final diagnosis, Germ tube test and Sugar absorption test (with API 120 kit) were used.

Results:  Of 120 patients, 61 (50.8%) were positive for Candida.  Candida infection rate was relatively higher in older patients and in women.  The rate was significant in the patients using dentures (P< 0.001) and   the ones keeping on smoking (P <0.038).  The infection in the patients with type II diabetes was higher than the cases with Type I.

Conclusion: Given the high prevalence of Candidiasis and Candida colonization in the mouth of diabetic patients, it is a necessity to identify and treat these infections in order to prevent further complications.

Keywords: Candidiasis, Diabetes Mellitus, Fungal Infections

Farzaneh Mohammadzadeh Rostami , Milad Shahsafi ,
Volume 12, Issue 6 (Nov - Dec 2018)

           Background and Objectives: Halophilic bacteria can grow and survive in environments with a wide range a wide range of salinities. In this study, we aimed to isolate halophilic bacteria from the Meyghan Lake in Arak (Iran) and evaluate their enzymatic activity.
           Methods: Samples were taken from four different areas of the lake. Halophilic bacteria were isolated by culture in moderate halophilic medium, sea water nutrient agar and nutrient agar containing different salt concentrations. Purification was done via consecutive culture methods, and production of extracellular hydrolytic enzymes including amylase, protease, lecithinase, DNase and lipase was evaluated.
           Results: Among 74 bacteria isolated from the lake water samples, 24 produced amylase, 27 produced lipase (Tween 40 and 80), 68 produced protease, three produced DNase and 61 produced lecithinase.
           Conclusion: In this study, we isolated halophilic bacteria with enzymatic activity and potential industrial applications.
           Keywords: Biological Diversity, isolation and purification, Halobacteriales, Meyghan Lake, Arak.

Farzaneh Mohammadzadeh Rostami, Saman Shalibeik, Morteza Rabi Nezhad Mousavi,
Volume 14, Issue 1 (Jan-Feb 2020)

          Background and objectives: Nosocomial infections caused by antibiotic resistant bacteria is a life threatening health challenge. This study aimed to determine the frequency of antibiotic resistance genes in clinical isolates from hospitals of Zahedan, southeast of Iran.
           Methods: Overall, 818 isolates were collected from different hospital wards. The isolates were identified using conventional microbiological and biochemical tests. Antibiotic susceptibility pattern was assessed by agar disc diffusion method and determination of minimum inhibitory concentration of a number of antibiotics. Multiplex PCR was performed using specific primers for the detection of resistance genes.
           Results: The most common species were Staphylococcus aureus (25%), Klebsiella pneumoniae (22%) and Pseudomonas aeruginosa (14%). The rate of methicillin resistance among S. aureus, S. epidermidis and S. saprophyticus was 60%, 43% and 24%, respectively. In addition, 28.5% of enterococci isolates were vancomycin resistant. Among gram-negative bacteria, 45% of A. baumannii and 24% of P. aeruginosa were identified as ESBL. A high level of resistance to ampicillin (96%), cefotaxime (89%), gentamicin (89%) and sulfamethoxazole-trimethoprime (60%) was observed in K. pneumoniae.
           Conclusion: Our results highlight the urgent need for an eradication program and a surveillance plan for preventing increased emergence of antibiotic resistant bacteria in the study area.
           Keywords: Bacterial Infections, Drug resistance, Zahedan.

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