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M Pourhossein, I.s Roberts,
Volume 2, Issue 1 (Spring - Summer 2008[PERSIAN] 2008)


Background and objectives:

important virulence factor for many invasive bacterial pathogens of humans.

Escherichia coli offer a model system to study the mechanisms by which

capsular polysaccharides are synthesized and exported onto the cell surface of

bacteria. Biosynthesis of the E

consists of the repeat structure -4) GlcA- (1, 4)-GlcNAc- (1-, requires the

KfiA, KfiB, KfiC, and KfiD proteins. Until now , the role of all proteins

except KfiB have been kown.

Capsular polysaccharide expression is an. coli K5 capsular polysaccharide, which

Material and Methods

(pMA1) and several derivatives of KfiB expression construct (pMA2-6) were

made with a 6Xhis-tag at the N-terminus. The presence of the hexa histidin tag

facilitated purification of the KfiB protein using Ni

: To study the role of the KfiB protein, a full-length2+-NTA chromatography.


pPC6::23 and restore capsule production. Successful complementation by

pMA1, showed that the fused hexa-histidine tag did not interrupt the function

of KfiB and that the full-length KfiB is required for complementation.

All plasmids except pMA1 failed to complement the mutation in

Roberts, I.S.


cytoplasmic membrane.

Localization studies revealed that KfiB is associated with the

Key words:

Escherichia coli, Capsular polysaccharide, KfiB, K5

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