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Showing 7 results for Pordeli

S M Hashemi, A Nasrollahi Omra, Hr Pordeli, A Hosenian,
Volume 4, Issue 2 (Autumn – Winter 2011[PERSIAN] 2010)

Abstract Bachground and objectives:Streptomyces is the most important genus in Actinomycetes family.The Streptomycetes are widely used in industry producing numerous chemical compounds including antibiotics, enzymes and anti- tumor agents. The aim of this study was to isolate soil-borne Streptomyces producing antimicrobial substances from soil of Golestan province of Iran and to survey anti-fungal metabolities produced by this organism. Material and Methods:In this study various soil samples were collected: ( forest areas of Naharkhoran in Gorgan and Kordkuy’s Derazno, Aghala’s deserts and agriculture lands of Aliabad ) were cultured on Actinomycet isolation agar and Starch casein agar were identified and purified by morphology and biochemistry tests. The activity of isolated Streptomyses against:Aspergillus flavus, Aspergillus, Candida albicans and Malasesia fur fur were studied by Agar Diffusion. Results:Of 120 samples, 24 are Streptomyces(20%) .The frequency of Streptomyces are reported in Aghala (10,41.6%),Derzno (8,33.3%) ,Nahar khoran(4,16.6%) and Aliabad(2,8.3%).Of 24 isolated Sterptomyses,two isolates have strong anti-fungal and six of them have moderate effect.We also see Streptomyses,isolated from desert area, have higher anti-fungal activity. Conclusion:It is recommended two isolated of Streptomyses be identified ana purified. Key words:Streptomyces , antifungal activities, antibiotic

Hr Pordeli, Sj Hashemi Hazaveh, M Jamshidian, M Bayat,
Volume 7, Issue 2 (summer[PERSIAN] 2013)

Abstract Background and objective: Soil bacteria, particularly Bacillus genus have the potential of producing a range of bioactive substances with antimicrobial and antifungal properties. They have the ability to produce hundreds of active and effective biologic compound against microorganisms. Therefore, it seems to be a proper candidate in the biocontrol of fungal pathogenesis. Material & Methods: In this study, soil samples were collected from different parts of Gorgan in order to isolate Bacillus and to determine their antifungal activity against T.mentagrophytes. The Isolates that had the highest antifungal effects were analyzed by PCR and 16s rRNA sequencing. Results: of 54 strains, 14 have antifungal activity. The Isolates, S4 and S12, identified as B.cereus and B.thuringiensis respectively show the highest antidermatofit effect. These isolates based on 16s rRNA sequence analysis show 97% homology with Bacillus cereusstrain KU4 and Bacillus thuringiensisstrain ucsc27. Conclusion: According to the results, it seems that the soil Bacilli have biocontrol potential against dermatophytic agents such as T.mentagrophytes. Keywords: Antifungal effects, Bacillus, Rhizospheric soil, T.mentagrophytes
A Shariati, Hr Pordeli, Me Tajari, E Yazarloo, S Kaghazloo, N Ebrahimi,
Volume 7, Issue 3 (Autumn 2013)

Abstract Background and Objective: Understanding the resistance patterns of bacteria and their sensitivity and attempting to find new compounds with broad effective spectrum would be significant in controlling infection. This study aimed at evaluating antibacterial potential of three honey samples produced in Golestan province, Iran, against Enterococcus faecalis strains. Material and Methods: After the isolation and identification of bacterial strains, their antibiotic resistance was determined using Kirby-Bauer method. Then, seven isolates of Enterococcus faecalis with multidrug-resistance were selected and antibacterial activities of honey samples assessed by disk-diffusion, well-diffusion and minimum inhibitory concentration test (MIC). Results: The highest diameter of inhibition zone in disk-diffusion and well diffusion methods is 20 mm and 26 mm, respectively. Also the MIC is measured 62.5 for all samples. Conclusion: In prohibiting microbial growth, all three samples were successful and they could prevent growing bacterial infection in spite of remarkable resistance of Entrococcus. Hence, further research should be conducted to assess the effects of honey samples against other bacteria. Keywords: Multidrug-Resistance Enterococcus spp Honey Golestan Province
Aj Eiri, Aa Nasrollahi Omran, Hr Pordeli,
Volume 7, Issue 5 (supplement Issue( Bacteriology)[PERSIAN] 2014)

Abstract Background and Objective: Chitin, which is a linear polymer of N-acetyl glucosamine residues, has been the most abundant polymer in nature after cellulose. In recent decades, Chitinases have received increased attention because of their wide range of applications, especially in biological control against fungi. Material and Methods: the isolation of bacilli producing chitinolytic enzymes was performed by collecting 40 soil samples from various regions of Gorgan, northern of Iran. The chitinolytic potential of the isolates was indicated by observation of clear zone in colloidal chitin agar medium. Identification of selected strains was performed by polyphasic taxonomy, and subtler identification and sequensing were carried out by extraction DNA. Antifungal effect was evaluated by well method against Candida albicans (ATCC 10231) Aspergillusniger (ATCC 2029)،Aspergillusflavus (IR6) Fusariumoxyporum (PTCC 5115) and Alternariaalternata (PTCC 5224). Results: Nine colonies of chitinase positive bacillus were isolated on choloidal Chitin Agar (CCA) and five of them had antifungal effect. R6 strain had the highest, and R2 and R3 had the lowest effect on fungi. The 16S rRNA sequence of these isolations in comparison with the known bacteria has 95-97% similarity. Conclusion: Some of the soil bacteria can have antagonestic effects on human and phytopathogenic agents existed in soil. Keywords: Bacillus Chitinase Soil Antifungal
Hamidreza Pordeli, Hadi Shaki, Ania Ahani Azari, Muhammad Sadeqi Nezhad ,
Volume 12, Issue 4 (Jul-Aug 2018)



            Background and objectives: Silver nanoparticles (AgNPs) are major nanomaterials with a variety of applications. The synthesis of nanoparticles by conventional methods is challenging and often requires use of hazardous chemicals. Therefore, there is a growing need for development of environmentally and economically friendly processes for the synthesis of nanoparticles. This study aimed at biosynthesis of AgNPs using a filamentous fungus; Fusarium solani.
            Methods: Twenty-four Fusarium isolates were found from several soil samples collected from depth of 1-10 cm. All isolates were identified as F. solani based on morphological characteristics. The synthesis of nanoparticles were evaluated after 24, 48, 72 hours of culture. AgNPs were characterized using UV-visible spectroscopy and transmission electron microscopy.
            Results: The synthesized AgNps showed maximum absorbance peak at 420 nm after 72 hours. Moreover, most AgNps were spherical with diameter of between 20 and 40 nm.
            Conclusion: In this study, we introduced a simple biological process for biosynthesis of AgNPs using F. solani isolates from soil samples. The results indicate that fungi may be suitable for safe and cost-effective production of AgNPs.
            Keywords: Fungi; Fusarium; Nanoparticles; Nanotechnology.

Mohammad Habibi Juybari , Hamidreza Pordeli , Saeid Mikaeili ,
Volume 13, Issue 3 (May-Jun 2019)

            Background and Objectives: Schiff base ligands are prepared via the condensation reaction of 1, 10- dimethyl–phenantroline aldehyde derivative with some nitrogen donor ligands, such as benzene ring that have different functional groups (-OH, -SH, -OCH3,-CH2OH, -Br) in acetonitrile. Recent studies suggest that Schiff bases might have antibacterial activity. Therefore, we aimed to synthesize new Schiff base complexes and evaluate their antibacterial activity against a number of Gram-positive and Gram-negative bacteria.
            Methods: Schiff base ligands and their complexes were characterized by mass spectrometry, infrared spectroscopy and nuclear magnetic resonance spectroscopy. The in vitro antibacterial activity of the Schiff base ligands and metal ions against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa was evaluated by determining minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) using the broth dilution method.
            Results: All synthesized Schiff bases exhibited favorable antibacterial activity against the tested microorganism, but the antibacterial effect of compounds 3OH and 3SH was more significant than that of other compounds.
            Conclusion: Compound 3EOH has favorable antibacterial activity against the tested bacteria.
            Keywords: Schiff bases, antibacterial effect, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa.

Leila Fozouni, Hamideh Askari, Hamid Reza Pordeli,
Volume 13, Issue 4 (Jul-Aug 2019)

            Background and Objectives: Enterococcus faecalis is a major cause of bacterial prostatitis, which can increase the risk of developing prostate cancer if mistreated or left untreated. The aim of this study was to evaluate resistance of E. faecalis strains isolated from patients with prostatitis to three fluoroquinolones.
            Methods: In this cross-sectional study, we collected urine specimen from 164 patients hospitalized in six hospitals in the Golestan Province, Iran. Biochemical and bacteriological tests were carried out to identify E. faecalis strains. Pattern of resistance to ciprofloxacin, levofloxacin and norfloxacin was studied using the agar disk diffusion method (Kirby-Bauer method). The broth microdilution test was performed to determine minimum inhibitory concentrations (MICs) of fluoroquinolones according to the CLSI M100-S25 (2015) criteria.
            Results: Of 164 isolates, 39 (23.8%) were identified as E. faecalis. Frequency of resistance to ciprofloxacin, norfloxacin and levofloxacin was 12.8%, 12.8% and 2.6%, respectively. The MIC90 of ciprofloxacin against the isolates was 4 μg/ml, which was 4-fold lower than that of norfloxacin (MIC90=16μg/ml) and 2-fold lower than that of levofloxacin (MIC90=8μg/ml). We found no significant difference between the isolates in terms of resistant to the fluoroquinolones (P>0.01). 
            Conclusion: Our results show that E. faecalis is one of the most common causes of bacterial prostatitis, and fluoroquinolones are still effective for treating the infection despite the reports of fluoroquinolones resistance in Iran. Moreover, levofloxacin may be a more suitable and effective antibiotic than ciprofloxacin and norfloxacin for treatment of this infection.
            Keywords: Enterococcus faecalis, Prostatitis, Drug Resistance, Iran.

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