Showing 3 results for Nasrolahi Omran
Nasrolahi Omran A, Bay A, Poorshamsian Kh, Karimi Kh, Hashemi M, Maghsodlo B,
Volume 5, Issue 1 (spring-summer[PERSIAN] 2011)
Abstract
Abstract Background and objectives: Microbial, physical and chemical properties of drinking water, considering type and quantity of the drinking water, are the factors for determining the acceptability of water. The effect of each of the above components on increasing the quality of water or threatening the health of consumers is also considerable. We aimed to investigate the trace amounts of microbial, physical and chemical parameters of drinking water in Gorgan, comparing to national and international standard. Material and Methods: In this cross sectional study, 50 samples were taken, per month, from four pilot stations in urban water network of Gorgan during one year (2009- 2010). We used instant sampling and performed Enumeration of Coliforms in water culture with most probable number (MPN).Samples taken from the stations during the month were investigated in two general categories of device test and heading-meter. All experiments were performed using standard reference method. Results: of 598 water samples 551 (92.2%) have not Coliforms, 47 (7.8%) contain Coliforms and 16 (2.7%) of them have thermo-tolerant Coliforms. Mean water hardness is 413mg/L, base on carbonate calcium. The amount of fluoride about 0.35 mg and the residual chlorine about 0.6 mg is less than national standard amount. Conclusion: The results show that Gorgan water network is, in view of microbial contamination, safe during almost all days. All the tested samples in terms of physical and chemical parameters are in the range of nation and international standards. Hardness of drinking water of Gorgan is one of the highest. Key words: Bacteriological, Physiochemical, Drinking Water, Gorgan.
S Ahmady- Asbchin, A Nasrolahi Omran, N Jafari, Mj Mostafapour, S.m Kia,
Volume 6, Issue 2 (Autumn- Winter [PERSIAN] 2012)
Abstract
Abstract
Background and Objectives: Concurrent with the development of new chemical drugs and antibiotics, their harmful effects are gradually emerged. Due to lack of harmful effects, herbal medicines have been used in the pharmaceutical industry. The aim of this study was the use of lavender essential oil as an herbal medicine for the replacement of antibiotics and chemicals.
Material and Methods: In this study, the plant essential oil was isolated by drying and distillation method using Clevenger apparatus. The antibacterial effect of this plant was evaluated by using disc diffusion method and successive dilutions. In order to control the standard of the method, antibiotic discs and standard bacterial strains were used.
Results: Based on the results, Proteus mirabilis and Enterococcus faecalis are , respectively , the most sensitive and most resistant bacteria to dilutions of 1, 1/2 and 1/4. Escherichia coli and Enterococcus faecalis, respectively, are the most sensitive and most resistant bacteria to the dilution of 1/8, 1/16, 1/32 and 1/64. MIC and MBC methods also show that all bacteria have the same minimum inhibitory and fatality concentrations except Enterococcus faecalis with minimum inhibitory concentration of 16/1 and minimum concentration fatality of 8/1. Evaluating the results of the disk diffusion method with antibiotic discs, we can observe the better effect of this plant in comparison with gentamicin and streptomycin discs on the growth of five strains of Staphylococcus aureus ATCC1885, Staphylococcus epidermidis ATCC 2405, Enterococcus faecalis ATCC2321, Escherichia coli ATCC 1652 and Proteus mirabilis ATCC 2601.
Conclusion: the essential oil of Lavender can be used instead of chemical drugs to treat bacterial infections.
Keywords: Lavandula, Anti-bacterial effects, Essential oils, Bacterium
Zahra Khozein , Ayatollah Nasrolahi Omran , Aylar Jamali ,
Volume 9, Issue 5 (Nov,Dec-2015 2015)
Abstract
Abstract
Background and Objective: the Formation of urinary infection by uropathogenic E.coli needs numerous virulence factors and biofilm formation is among these factors. Bacteria that form biofilms express phenotype traits that appear according to the bacteria type. Cellulose is an important compound on the outside of E.coli causing bacterial cell-cell reactions and connection to nonliving surfaces. Curli pili cause the reaction between cell-cell and surface-cell in biofilms and lead to bacteria aggregation. Microorganisms’ ability to form biofilm on a surface depends on the surface nature and its conditions. This study aimed at determining the production ability of cellulose polysaccharide and curli pili in UPEC strains, and its correlation with formation and intensity of biofilm.
Methods: In this study carried out to compare the ability of cellulose and pili curli production ability in 40 uropathogenic E.coli isolates ,by morphotype method in Congo Red medium (CR), each isolate was incubated at 37 oC, for 24 hours. After 24 hours, all colonies’ morphology characteristics were studied
Results: It was shown that 67.5% of strains produced cellulose and 72.5% produced curli pili. In addition, 92.6% and 89% of isolates that produce cellulose and curli, respectively, had a moderate to strong biofilm. Moreover, it was shown that there is a significant correlation between cellulose and / or curli pili production with biofilm intensity.
Conclusion: About 70% of E.coli isolates from patients' urine are able to produce cellulose or curli pili; therefore, it can be concluded that the production of these two combinations is effective in amount and intensity of biofilm formation.
Keywords: Escherichia coli; Cellulose Polysaccharide; Curli Pili; Biofilm.
