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Showing 3 results for Najar

Nazari, H, Habibeh Najar, H, Emadi, A, Abasi, Y, Salehiyan, A, Monem, M, Ghods, F, Mohammadi, A., Khaleghian, A,
Volume 5, Issue 2 (Autumn – Winter 2011[PERSIAN] 2011)
Abstract

Abstract Background and objectives: Glucose-6-phosphate dehydrogenase (G6PD) is an enzyme in the pentose phosphate pathway. G6PD deficiency (an X-linked recessive hereditary disease) is an inherited condition affecting approximately 3% of the people globally. This deficiency can cause hemolytic anemia and jaundice in neonates. The goal of this study is to detect the prevalence of G6PD deficiency in neonates referred to Semnan province screening lab Material and Methods: This cross sectional study, from 2007 to 2010, was conducted on the basis of country planned program and in line with neonatal screening tests. Blood samples were taken from the heels of 3-5 day neonates. Assessment of G6PD was done by rapid fluorescent spot test. The Prevalence of G6PD deficiency in boys and girls was compared by chi square (p<0.05(test. Results: Of 9353 newborns referred to semnan province screening Lab., 4820(51.53%) are males and 4533(48.47%) females. Three hundred (3.2%) of them suffer from G6PD deficiency. Of these, 263 (5.45%) are males and 37 (0.81%) females. The ratio of male to female is 7 to 1. Conclusion: The prevalence of G6PD deficiency is detected 3.2% in Semnan province and its frequency in boys is more than that of girls, which is expected. Key words: Glucose -6-phosphate dehydrogenase, Neonatal screening, Fauvism
Derakhshan, S, Najar Peerayeh, Sh, Fallah, F, Bakhshi, B,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract

Abstract Background and Objective: Multiple drug resistance has increased in recent years in Klebsiella pneumoniae isolates. The Integrons are mobile genetic elements that carry antibiotics resistance genes. The aim of this study was to determine antibiotic susceptibility and the prevalence of class 1, 2, and 3 integrons in clinical Klebsiella pneumoniae isolated from clinical specimens. Material and Methods: A total of 108 K. pneumoniae isolates were collected between April and December 2011 from different clinical specimens of Loghman hospital in Tehran and identified by biochemical tests. Susceptibility of isolates to 14 antibiotic disks was determined by disk diffusion method. The template DNA was extracted by freeze-thaw method and the presence of class 1, 2, and 3 integrons was investigated by PCR method. Level of resistance to antibiotics in integron-positive and integron-negative isolates was determined. Results: The highest level of resistance was seen for cefotaxime, ceftriaxone, and amoxicillin-clavulanic acid (55.5%). In 79 isolates (73.14%) class 1 integron and in 57 of 79 isolates (72.15%) resistance to at least two classes of drugs were seen. The class 2 and 3 integrons were not detected. Among integron-negative isolates, 8 isolates (27.58%) had resistance to at least one antibiotic. Conclusion: The prevalence of class 1 integron in resistant K. pneumoniae is high therefore, the monitoring of drug resistance and limiting the use of antibiotics are necessary. Keywords: Klebsiella Pneumoniae, Integron, Multi-Drug Resistance


Shima Najar, Nafiseh Sadat Naghavi ,
Volume 12, Issue 4 (Jul-Aug 2018)
Abstract

ABSTRACT

           Background and Objectives: Hepatitis A virus (HAV) enters sewage through the stool of infected patients and contaminates freshwater and seawater. The aim of present study was quantitative molecular detection of HAV in raw and treated urban sewage samples from Isfahan, center of Iran.
           Material and methods: Sewage samples were collected from six urban treatment plants in cold and warm weather, between December 2016 and Jun 2017. Physicochemical indicators were measured immediately after sampling. Viral particles were isolated and concentrated by modified adsorption-elution method using Al3+-coated filters. Quantitative PCR was done for amplification of a fragment in the highly conserved 5′ noncoding region of the HAV genome.
           Results: Although the values of the indicators were lower in 67-83% of sewage samples in warm weather, no significant difference was detected between the physicochemical indicators of sewage samples. HAV was detected in three raw sewage samples collected in cold weather (copy number of 32590, 28400 and 14200 per µl) and in one treated sewage sample (copy number of 6500 per µl) collected in cold weather. Positive HAV sewage samples had been treated by activated sludge and aerated lagoon.
           Conclusion: We detected high frequency of HAV in raw urban sewage samples from Isfahan, Iran. This highlights the importance of paying extra attention to irrigation of farms with raw sewage. Based on the results of this study and previous studies, it can be concluded that the activated sludge system may be more effective for elimination of enteric viruses, such as HAV.
           Keywords: Hepatitis A virus, Sewage, Weather.


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