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Showing 4 results for Nadi

Ms Shirakdehi, M Rezaei, E Nadi, H Mahjoub, Mt Goodarzi,
Volume 8, Issue 1 (spring[PERSIAN] 2014)
Abstract

Abstract Background and Objective: Oxidant-antioxidant imbalance plays a key role in pathogenesis of chronic obstructive pulmonary disease (COPD). This study aimed to evaluate homocystiene and total antioxidant capacity in COPD patients, compared to smoker and non-smoker healthy people. Material and Methods: We measured total antioxidant capacity with Cayman Kit, uric acid with Pars Azmoon kit٫ homocysteine with ELISA Kit and inflammatory cells (leukocytes) in 29 COPD patients, 29 smokers and 29 non-smokers. Results: Uric acid was significantly higher in COPD patients compared to healthy smokers and healthy non-smokers (p<0.05). Total antioxidant capacity was significantly lower in COPD patients compared to healthy, non smokers (p=0. 003). In COPD patients, homocysteine and leukocytes levels were significantly higher than those in healthy smokers (P<0.05) and healthy non- smokers (p<0.001). Conclusion: According to high inflammatory cells and low antioxidant capacity in COPD, early administration of appropriate medication is recommended to reduce systemic and topical inflammation. Reduction in the exposure to oxidizing compounds can slow the process of degradation and damage to lungs. Keywords: Chronic Obstructive Pulmonary Disease Homocysteine Oxidative Stress
Asghari Estiar, M, Rafi, A, Heidarzadeh, S, Ohadian Moghadam, S, Mahboubi, R, Monadi Sefidan, A, Allafzadeh, J, Nik Khah, H,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract

Abstract Background and Objective: One of the main causes of increased mortality in cancer patients is bacteremia. On the other hand, antibiotic resistance is the major cause of treatment failure in malignant diseases especially in hematological malignancies. The aim of this study was to diagnose the bacterial strains isolated from blood specimens of cancer patients and to determine their antibiotic susceptibility. Material and Methods: In this cross-sectional study, 0.5 ml of venous blood was taken from 613 cancer patients especially leukemia, and blood cultures and antibiotic susceptibility tests were performed using standard methods. Using disc diffusion method, antibiotic susceptibility was performed with a wide range of antibiotics. Results: Out of 613 cultured specimens, 153 (25%) were found to be positive including 76.47% of gram negative and 23.53% of gram positive bacteria. The most common isolated bacteria were E. coli, coagulase-negative Staphylococci, Klebsiella, Staphylococcus aureus and Pseudomonas aeroginosa, respectively. Conclusion: It seems that Ceftriaxone is the best choice for the treatment of gram negative caused bacteremia and Gentamicin for bacteremia caused by gram positive agents. Given the high level of resistance to the commonly used antibiotics, it seems reasonable to avoid of early and inappropriate use of antibiotics to prevent the development of drug resistant bacteria. Keywords: Cancer, Blood Cultures, Bacteremia, Antibiotic Resistance


Monadi, M, Kargar, M, Naghiha, A, Najafi, A, Mohammadi, R,
Volume 9, Issue 1 (March, April[PERSIAN] 2015)
Abstract

Abstract Background and Objective: Salmonellosis is the most common type of food poisoning in developed and developing countries that is caused by Salmonella serotype. Hence, we aimed to identify the Salmonella serovars in eggs obtained from Kohgiluyeh and Boyerahmad province and to evaluate antibiotic resistance of the isolated strains. Material and Methods: In this study, 210 eggs were collected from Kohgiluyeh and Boyerahmad Province. The bacteria were isolated and identified using biochemical tests. After extraction of genomic DNA, Salmonella gender, Salmonella enteritidis and Salmonella typhimurium were investigated by invA, fliC and sefA primers, respectively, using Multiplex PCR method. Results: Of 210, 14 (6.66%) were contaminated with Salmonella. Of these, 12 (5.71%) were Salmonella typhimurium and 2 (0.95%) were related to Salmonella spp. None of the samples were contaminated with Salmonella enteritidis. The highest resistance was related to penicillin (100%) and neomycin (78.57%). Conclusion: Salmonella typhimurium is the predominant serovar causing contamination in the eggs of this Province. Given the wide spread of antibiotic resistance in different serotypes of Salmonella, we recommend avoiding of indiscriminate use of antibiotics in livestock and poultry. Keywords: Salmonella, Drug Resistance, Antibiotic, Multiplex PCR, Kohgiluyeh and Boyerahmad
Shadi Beladi Ghannadi , Maryam Ghane , Laleh Babaeekhou ,
Volume 13, Issue 2 (Mar-Apr 2019)
Abstract

ABSTRACT
             Background and Objectives: The emergence of extended-spectrum β-lactamase (ESBL)-producing Shigella spp. is becoming a health concern worldwide. This study aimed to investigate antibiotic resistance pattern and frequency of blaCTX-M, blaSHV, and blaTEM genes among Shigella isolates from patients in hospitals of Tehran, Iran.
             Methods: In this cross-sectional study, 52 non-repeated Shigella strains were isolated from hospitalized patients in Milad, Emam Khomeini and Shariati hospitals in Tehran (Iran) from November 2015 to December 2016. Bacterial identification, serotyping, and antimicrobial susceptibility testing were performed according to the standard guidelines. The blaCTX-M, blaSHV, and blaTEM resistance genes were identified using multiplex polymerase chain reaction.
             Results: Among 52 Shigella isolates, S. sonnei (44.2%) was the predominant species, followed by S. flexneri and S. dysenteriae (23%). Over 67% of the isolates were multidrug resistant. The highest rates of resistance were observed against cefalotin (67.3%), tetracycline (67.3%), amikacin (63.5%), trimethoprim-sulphamethoxazole (48.1), and ampi­cillin (42.3%). The lowest resistance rate was against ciprofloxacin (1.9%). We detected the blaTEM and blaCTX-M genes in 61.5% and 19.2% of the isolates, respectively. However, the blaSHV gene was not detected in any of the isolates. In addition, 16.4% of the isolates harbored the blaTEM and blaCTX-M genes simultaneously. Ciprofloxacin was the most effective antibiotics according to the ESBL genes distribution.
             Conclusion: Our findings indicate the high prevalence of multidrug resistance and ESBL genes in Shigella isolates, which elucidates the need for appropriate infection control measures for limiting the spread of resistant strains.
             Keywords: Shigella, Multiplex Polymerase Chain Reaction, Drug Resistance.


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