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Amir Mohammadi , Masoume Mazandarani , Jila Asghari (phd),
Volume 10, Issue 3 (May-Jun 2016 2016)
Abstract

ABSTRACT

          BACKGROUND AND OBJECTIVE: Stachys inflata Benth. is used as an anti-inflammatory and antiseptic agent in traditional medicine in most mountain villages of Golestan province. Therefore, this study aimed to investigate the antioxidant, ethnopharmacological and phytochemical properties of extract from different parts of S. inflata, collected from Chahar Bagh Mountain.

         METHODS: Flowering branches and root of the plant were collected from Chahar Bagh Mountain (2100 m) in July 2013. At the same time, the most important information about traditional uses of the plant (ethnopharmacology) was recorded by questioning local people. Phytochemical evaluation (total phenolic, flavonoid and anthocyanins content) of ethanolic extract of plant organs was done using spectrophotometry and folin-ciocalteu. The antioxidant activity of the extract was evaluated by DPPH test. P ≤0.05 was considered as statistically significant.

       RESULTS: The amount of chemical compounds in the extract of flowering branches and root extract was significantly different. The total phenolic (129.96 ± 5.6 mgGAE/g), flavonoid (29.62 ± 1.4 mgQUE/g) and anthocyanin (0.021 ± 0.001 µg/g) content in the extract of aerial parts of the plant was approximately 1.5 to 3 times higher than those in the root. Due to higher production of active compounds, the antioxidant activity of the aerial parts’ extract showed  a greater potential in free radical scavenging (IC50= 76.33 ± 4.2 µg/ml) compared to the root extract.

        CONCLUSION: Phytochemical findings and antioxidant activity of the extract of aerial parts of the plant in free radical scavenging, confirm the traditional applications of this plant as analgesic, anti-inflammatory and antiseptic agent in treatment of rheumatism, wounds, burns and diarrhea. It is recommended that further evaluation of the plant’s traditional applications be conducted in vivo and in vitro.

       


Seyedeh Masoumeh Mirtaghi, Parisa Torbati Nejad , Masoumeh Mazandarani, Fasiheh Livani, Hanieh Bagheri ,
Volume 10, Issue 5 (Sep-Oct-2016 2016)
Abstract

Background and Objective: Nowadays, incidence of antibiotic-resistance among pathogenic bacteria has increased due to indiscriminate use of antimicrobial drugs for treatment of diseases, especially urinary tract infections. Medicinal plants are also of great importance as antibacterial agents. Therefore, the aim of this study was to determine the antibacterial effect of ethanolic extract of nettle (Urtica dioica L.) leaves using two methods of disk diffusion and well diffusion.

         Methods: Ethanolic extract of nettle leaves was prepared by the percolation method. Effect of different concentrations of the extract on Escherichia coli (PTCC1399), Staphylococcus aureus (PTCC 1431), Staphylococcus epidermidis (PTCC 1435) and Staphylococcus saprophyticus (PTCC1440) was evaluated using the disk diffusion and well diffusion methods by measuring diameter of growth inhibition zone. Gentamicin and propylene glycol were used as positive and negative control, respectively.

        Results: In both methods, especially in the well diffusion, the ethanolic extract of nettle leaves had favorable inhibitory effect on the growth of S. aureus, S. epidermidis and S. saprophyticus. In the well diffusion method, the highest rate of susceptibility to the extract (89%) was related to S. saprophyticus and S. epidermidis.

         Conclusion: The ethanolic extract of nettle leaf has good inhibitory effect on the growth of S. aureus (especially in the well diffusion method), which confirms the traditional use of this plant for the treatment of urinary tract infections.

          Keywords: Antibacterial Effect, Staphylococcus, E. Coli, Ethanolic Extract, Nettle (Urtica dioica L.).


Masoumeh Mazandarani, Leili Monfaredi ,
Volume 11, Issue 1 (Jan-Feb- 2017 2017)
Abstract

ABSTRACT
          Background and Objectives: Many aromatic plants from the genus Satureja have been used in traditional medicine in north of Iran. This study aimed to determine the ecological requirements for the growth of Satureja mutica Fisch. & C.A. Mey, and evaluate antioxidant and antibacterial activity of ethanolic extract of S. mutica collected from North Khorasan Province, Iran.
         Methods: Aerial parts of S. mutica were collected in blooming stage. Ecological requirements and the traditional uses of the plant were recorded. Ethanol extract of the plant was prepared by maceration. Antioxidant capacity of the extract was measured by three methods of total antioxidant capacity, reducing power and 2,2-Diphenyl-1-picrylhydrazyl, and then compared with standard antioxidants (butylated hydroxyanisole and butylated hydroxytoluene). Antibacterial activity of the extract was studied against nine Gram-positive and Gram-negative bacteria by agar dilution method and determining the minimum inhibitory concentrations (MICs).
         Results: S. mutica is the most common wild aromatic annual herb in north slob and sunny areas around mountains of Bojnord (1020-1300 m). The ecological features of this region are as follows: annual rainfall 308 mm, average temperature 11.5 oC, semi dry cold climate in the sandy clay loam soil, Ec=0.7 desizimence, and pH= 7.30. Ethnopharmacological data showed that this plant has been widely used by rural people as an anti-infective, antispasm and sedative agent that could treat rheumatic pain, migraine, toothache and diarrhea. The ethanol extract of S.mutica had relatively high antioxidant activity with IC50 value of 11.2 mg/ml. The extract also had high antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus and Enterococcus faecalis, with inhibition zone diameters ranging between 15.1±0.5 and 27.7±0.8 mm and MIC values of 60, 68, 53 and 83 mg/ml, respectively.
         Conclusion: It can be concluded that the extract of S. mutica has favorable antibacterial and antioxidant activity, which could be used as natural anti-microbial agent for treatment of some infection diseases.
          Keywords: Antioxidant, Antimicrobial, Bojnord, Ecological Requirements, North Khorasan, BHT, BHA.

Masoud Sheikh , Hamid Reza Eshraghi, Masoud Khoshnia, Masoumeh Mazandarani, Abdolvahab Moradi,
Volume 11, Issue 4 (Jul-Aug 2017)
Abstract

ABSTRACT
         Background and Objectives: Capparis spinosa has been used in traditional medicine for various applications including treatment of liver disorders and cancer. We studied the effects of this plant on cell proliferation and morphological characteristics of PLC/PRF/5 liver cancer cell line.
        Methods: After preparing ethanolic extract of the plant, the inhibitory effect of the extract was assessed using MTT assay, and morphological changes were assessed by an inverted microscope.
       Results: C. spinosa ethanolic extract exhibited anti-cancer effects in a concentration-dependent manner. Half-maximal inhibitory concentration of the extract was 1051±4.21 mg/mL. Morphological changes including cell shrinkage, reduction of cell volume and nuclear condensation confirmed the inhibitory effect of C. spinosa on PLC/PRF/5 cells.
        Conclusion: According to the results of this study, extract of C. spinosa seems to be suitable for prevention and treatment of liver cancer. Further studies on animal models could verify the efficiency of the extract against cancer cells.

KEYWORDS: Plants, Medicinal, Capparis spinosaLiver Neoplasms.       



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