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Showing 3 results for Maroufi

Soltan Dallal. M.m, Rahimi Forushani, A., Sadigh Maroufi, S, Sharifi Yazdi, K,
Volume 5, Issue 2 (Autumn – Winter 2011[PERSIAN] 2011)

Abstract Bachground and objectives: Salmonella is one of the most important agents of gastrointestinal infection and diarrhea in our country. Misdiagnosis of these bacteria leads to cure failure. The aim of this study was to make a comparison between PCR and the API-20E and conventional biochemical tests carried out for the identification of Salmonella. Material and Methods: In this study 470 specimens taken from children, with acute gastroenteritis, referred to teaching hospitals called Imam, Shariati and children medical centre. The specimens were transferred to microbiology laboratory in public health school for identification of Salmonella with PCR and API-20E methods. Results: Of 470 specimens, 65(13.8%) are positive for salmonella in hospital laboratory, while 37 (7.9%) for API-20E and 39 (8.3%) for PCR are positive. The results of antibiotic sensitivity tests on 39 salmonella isolated from diarrhea specimens show that 73.3% of them are resistance to at least one of the sixteen antibiotics tested. Conclusion: Based on the the results, there is significant difference (P<0.05) between conventional method, API-20E and PCR Key words: Salmonella, conventional identification, molecular identification
Hamed Noormohammadi , Yahya Maroufi , Mansour Dabirzadeh , Abdolhossein Miri ,
Volume 11, Issue 6 (Nov - Dec 2017)

            Background and Objectives: Leishmaniasis is a public health problem caused by the protozoan Leishmania. Pentavalent antimonials are currently used for treatment of leishmaniasis, but they have serious side effects. Nerium oleander L. has been used in traditional medicine due to its various health-protective properties. This study aimed to investigate anti-leishmanial activity of N. oleander L. leaves extract against Leishmania major promastigotes and amastigotes in vitro.
            Methods: L. major promastigotes were cultured in RPMI 1640 medium supplied with 10% fetal bovine serum. Different concentrations were prepared from the extract and added to L. major promastigotes seeded in 96-well plates. Viability percentage was evaluated by direct counting and MTT assay after 24, 48 and 72 hours. To investigate the cytotoxic effect of N. oleander L. on L. major amastigotes, the plant extract was added to amastigotes cultured in intraperitoneal macrophages. The mean number of amastigotes was calculated by direct counting after 24 and 48 hours.
             Results: All concentrations of the extract significantly reduced the viability of promastigotes when compared with the controls. Half-maximal inhibitory concentration was estimated to be 22.21 µg/ml after 24 hours. Percentage of cytotoxicity in amastigotes exposed to 20 μg/ml of the extract was 53.61% and 53.27% after 24 and 48 hours, respectively. In addition, percentage of cytotoxicity in amastigotes exposed to 80 μg/ml of the N. oleander L. extract was 53.77% and 55.48% after 24 and 48 hours, respectively.
            Conclusion: The N. oleander L. extract exerts anti-leishmanial activity on L. major promastigotes in a time- and dose-dependent manner.
             Keywords: Leishmania major, Nerium.

Asghar Farghi Yamchi , Mansour Dabirzadeh, Yahya Maroufi,
Volume 12, Issue 5 (Sep-Oct 2018)

           Background and objectives: Leishmania major is a flagellated parasitic protozoan that causes cutaneous leishmaniasis. Pentavalent antimony compounds are considered the first-line drugs in the treatment of cutaneous leishmaniasis. However, the use of these drugs is associated with numerous limitations and side effects. Therefore, there is a need for herbal and natural alternatives for these compounds with fewer side effects. In this study, we evaluated the in vitro activity of methanol extract of Quercus infectoria (oak galls) against promastigotes and amastigotes of L. major.
           Methods: In this experimental study, the effect of 10, 100, 500 and 1000 µg/ml of methanolic extract of oak galls and 100, 500, 1000 and 10000 µg/ml of Glucantime was evaluated against L. major promastigotes using direct cell counting and MTT assay. Moreover, the effect of different concentrations of the extract and Glucantime was investigated on the mean number of amastigotes in macrophages after 24 and 48 hours. Data were analyzed using SPSS 16 and one- way analysis of variance.
           Results: The half-maximal inhibitory concentration of the oak gall extract and Glucantime was 75 µg/ml and 221 µg/ml after 24 hours, respectively. After 24 hours, the mean number of amastigotes per macrophage was lowest at concentrations of 1000 µg/ml of the extract (0.9) and 10000 µg/ml of Glucantime (0.85).
           Conclusion: Considering the inhibition of intracellular and extracellular growth of L. major, the oak gall extract might be used as an efficient and safe agent for treatment of cutaneous leishmaniasis.
           KEYWORDS: Leishmaniasis, Cutaneous, Quercus.

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