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Comparison of Serum Vitamin D and Interleukin-6 Levels in Patients with Preeclampsia and Healthy Pregnant Women
Negin Rezavand, Asad Vaisi-Raygani, Firozeh Vaisi, Maryam Zangneh , Azin Hoshiar , Fariborz Bahrehmand, Amir Kiani, Gholamabass Dinarvand ,
Volume 10, Issue 4 (Jul-Aug 2016 2016)
Abstract

ABSTRACT

        Background and Objective: Nutritional factors and activation of inflammatory pathways are thought to be involved in pathogenesis of preeclampsia in pregnant women. The present study aimed to compare the serum levels of vitamin D and interleukin-6 in healthy pregnant women with those of preeclampsia ones.

        Methods: This case-control study was performed on 120 healthy pregnant women and 120 women with preeclampsia referred to Imam Reza Hospital in Kermanshah. The serum levels of vitamin D and IL-6 were measured by ELISA method. The data was analyzed by SPSS software (version 20) using independent t-test, the P-value of <0.05 was considered as statistically significant.

        Results: There was no statistically significant difference between the mean level of vitamin D in the patients (37.64 ± 29.50 ng/ml) and the controls (40.06 ± 33.20 ng/ml). the serum level of IL-6 in patients with preeclampsia (21.71 ± 32.24 pg/ml) was significantly higher compared to that of  control group (15.04 ± 28.6 pg/ml) (P <0.001).

       Conclusion: Based on the findings of this study, inflammatory factors and cytokines such as IL-6 can be considered as risk factor for preeclampsia. However, more studies with larger sample sizes are required to further evaluate the association of vitamin D levels and risk of preeclampsia.

           Keywords: Pregnancy, Pre-Eclampsia, Vitamin D, Interleukin-6.


Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island
Maedeh Kiani Abri , Monir Doudi , Ali Mohammad Ahadi ,
Volume 12, Issue 3 (May-Jun 2018)
Abstract
ABSTRACT
          Background and Objectives: Keratinase is an enzyme commonly used in the production of detergents, cosmetics, drugs, leather, and other industries. Considering the high cost of traditional methods for decomposition of feather, hair, hooves, nails, and wool that contain high levels of keratin, their biodegradation with keratinase-producing bacteria can be a valuable solution. The present study aimed for isolation and molecular identification of keratinase-producing bacteria in Qeshm Island and Peyposht village in Iran.
          Methods: Water and sludge samples from the Qeshm Island and Peyposht village were collected. The bacteria isolates were screened for keratinase production using the Lowry method. Effect of pH and temperature was assessed on the production of keratinase and on the growth of the isolates. Colony-polymerase chain reaction was used for molecular identification of the isolates.
          Results: Bacillus berevis and Enterobacter cloacae were isolated in this study. Keratinase production in B. berevis was highest at pH 7.5 and 35 °C. In addition, the highest level of enzyme production by E. cloacae was observed at pH 7 and 37 °C.
          Conclusion: It seems that the bacterial strains isolated from sludge in the study area have relatively favorable keratinase production capacity.
          Keywords: Bacteria, Colony PCR, Identification, Keratinolytic protein, Sewage.
 
 

Evaluation of in Vitro Antimicrobial Effects of Aqueous Extract of Tribulus terrestris Against Oral Bacteria
Zahra Kiani, Pouria Mohammad Parast Tabas, Khashayar Khalilpour, Mahjoube Goldani Moghadam, Majid Zare_bidaki,
Volume 15, Issue 5 (Sep-Oct 2021)
Abstract

Background and objectives: Medicinal plants have long been considered as one of the most important pillars of traditional medicine. Existing challenges in the treatment of diseases, particularly infectious diseases, are major drivers for herbal medicine studies. Tribulus terrestris has been widely used in traditional medicine to treat various diseases. This study aimed to investigate in vitro antibacterial effect of the aqueous extract of T. terrestris on several oral bacteria.

Methods: In this experimental study, after preparing the aqueous extract of T. terrestris, minimum inhibitory and bactericidal concentrations (MIC and MBC) of the extract were determined against standard strains of Streptococcus mutans, Staphylococcus aureus, Klebsiella pneumoniae and Streptococcus pyogenes using the broth microdilution method. The experiments were repeated three times and the results were analyzed with SPSS 22 using the one-way analysis of variance (ANOVA) and LSD statistical tests with the significance level set at 0.05.

Results: The aqueous extract of T. terrestris had the highest inhibitory effect on S. pyogenes and S. mutans, and the difference between the MIC and MBC values was significant (P <0.05). However, no such effect was observed against S. aureus and K. pneumonia at concentrations below 50 mg/ml when compared to ampicillin and chlorhexidine.
Conclusion: The aqueous extract of T. terrestris has significant antibacterial effects against S. pyogenes and S. mutans. Therefore, it can be incorporated into topical formulations such as toothpaste and mouthwash products after further in vivo and toxicity experiments.

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