Search published articles

Showing 7 results for Khaki

A Ghazavi, M Khaki, N Mosakhani,
Volume 3, Issue 1 (Spring - Summer 2009[PERSIAN] 2009)

Abstract Background and objectives: Since Rubella is an infectious disease with a few clinical symptoms, it is hardly diagnosed. Especially in the first trimester of pregnancy, congenital rubella is one of the major causes of neonatal mortality and permanent disablement in children. The aim of this study was to determine the sero immunity Level of The single Female studentchr('39')s after national rubella vaccination program in Iran. Material and Methods: This descriptive seroepidemiologic study was conducted on 129 single medical students. After taking written consent form, blood Sample was drawn. The anti-rubella IgG titer was evaluated using ELISA method. Statistical analysis was performed using SPSS (version10). Results: All Subjects had protective titer of anti-rubella antibodies. One hundred and eighteen students (91.5%) were immunized by taking rubella vaccine in the national vaccination program and only 11(8.5%) students reported that they suffered from natural rubella infection in the past. Conclusion: Based on the results of our study, vaccination against rubella in national vaccination program along with natural immunity caused by natural rubella infection in children could produce a protective immunity against rubella. Key words: Rubella, ELISA, vaccination, medical students
A Khaki,
Volume 8, Issue 2 (summer 2014[PERSIAN] 2014)

Abstract Background and Objectives: Ciprofloxacin is a synthetic antibacterial agent belonging to Fluoroquinolone drugs affecting effectively on gram-negative bacterial infectious. The aim of this study was to assess the effect of ciprofloxacin in the spermatogenesis period. Material and Methods: The subjects were 20 male wistar rat randomly divided in to control (n=10) and experimental group (n=10), given 12.5mg/kg ciprofloxacin (soluble in drinking water) in spermatogenesis period. On the day of 28, the sperm was collected from cauda epididymis and sent for analysis. Results: Based on light microscopic observation and statistical analysis, the majority of seminiferous tubules of control group were healthy, in Sc 8-9 stage. But in test group, sertoli cell degeneration and absence of sex cells were confirmed, and in some parts, just basal layer of seminiferous tubule was remained in Sc 3-5 stage. Conclusion: Sex hormones (LH and FSH) and spermatogenesis (sperm count, motility and viability) were significantly decreased in test group compared to those of controls (P<0.05). Ciprofloxacin has some adverse effects on sperm related variables in 28 day period. Keywords: Ciprofloxacin, Sperm, Sex Hormones
A Zahedi, A Khaki,
Volume 8, Issue 3 (Autumn[PERSIAN] 2014)

Abstract Background and Objective: Omega-3s are considered essential fatty acids that cannot be synthesized by human body and should be obtained from foodstuff. This study aimed at evaluating the beneficial effect of Omega-3s ,exposed in Electromagnetic Field (EMF), on sexual behavior ,Serum total testosterone level, malondialdehyde (MDA) level and total antioxidant capacity(TAC) in male rats. Material and Methods: 32 Wistar rats (males=24, females=8) were allocated to four groups of Control, EMF, omega 3 and omega 3 & EMF. Estradiol benzoate was injected to the female rats subcutaneously. Then after taking biopsy from epididym and testis of each groups, tissue preparation was performed to look through via light microscope on 28th day of study. Serum MDA, TAC and Testestrone were measured in male rat by Radio Immune Assay (RIA) method. Results: The highest degree of testicular tissue destruction and MDA level were observed in electromagnetic field group and the lowest in omega 3 group. The sexual behavior, testosterone hormone and TAC level were lowest in electromagnetic field group and highest in omega 3 group. Conclusion: Based on the Results, administration of omega 3 can significantly lower the adverse effects of EMF and have beneficial influences on sexual behavior in male rat. KeyWords: Electromagnetic Field Effect (EMF), Rat, Omega 3, Sexual Behavior
Roohi, Z, Moradi Bidhendi, S, Khaki, P,
Volume 9, Issue 1 (March, April[PERSIAN] 2015)

Abstract Background and Objective: Leptospirosis is a zoonosis infectious disease that is prevalent in tropical and subtropical regions and is caused by the pathogenic serovars of leptospires. Hence, we aimed at investigating the prevalence of antibodies against these bacteria in the blood samples of suspected leptospirosis. Material and Methods: the human serum samples (N = 130) were obtained from patients clinically suspected leptospirosis. The Serum level of IgM antibodies were studied by ELISA kit (PrioCHECK) in Razi Vaccine and Serum Research Institute (Karaj), 2011-2012. Results: Anti-leptospira IgM class was observed in 21(16%) samples. The relative distribution of the disease was reported in men (80.95%), women (19.04%), and farmers (30.95%) and in 20-40-year group (57.14%). Contact with contaminated water was the most common cause of infection (52.38%) and fever was the most common sign of Leptospirosis (72.2%). Conclusion: Due to the occurrence of anti-leptospira antibodies in 16% of suspected cases, it is recommended that routine ELISA be done at least in major diagnostic centers. Keywords: Leptospira, Leptospirosis, Human, ELISA
Arash Khaki ,
Volume 9, Issue 4 (sep,Oct 2015 2015)


         Background and Objective: According to Iranian, Asian, Indian, and Chinese traditional medicine, basil is used in the treatment of different diseases. In this study, the effect of basil on lowering the blood glucose was examined in healthy and diabetic rats.

        Methods: Sixty Wistar rats were divided into a control group(n=10), a basil receiving group (n=20) and a diabetic group (n=30)(60 mg/kg Streptozotocin- Intraperitoneal injection) and the rats in each group received basil orally in pairs. Forty-eight hours after injection of streptozotocin, 2 and 4 mg/kg doses of basil were prescribed orally for the diabetic group daily for 45 days. in end of study for record serum testosterone ,5 cc blood was sampled from each rat.

       Results: There was a significant correlation between the oral administration of 2 and 4 mg/kg doses of basil and reduced blood glucose level and testosterone changes in the diabetic groups (P<0.05). Twenty-four hours after the first administration, basil caused a significant decrease in the blood glucose level and this  effects continued for 24, 48, and 72 hours, respectively. In contrast, basil had no hypoglycemic effect in the healthy rats.

         Conclusion: The results indicated that 2 and 4 mg/kg doses of basil had significant hypoglycemic effects on the diabetic groups and compensated for the testosterone level in diabetic rats, but had no effect on the healthy rats.

        Keywords: Streptozotocin, Testosterone, Ocimum basiilicum, Diabetes Mellitus.

Younes Anzabi, Arash Khaki,
Volume 10, Issue 6 (Nov-Dec-2016 2016)


         Background and Objectives:  Many species of  Ziziphora have been used in traditional medicine in the Azarbaijan region, northwest of Iran. This study aimed to determine antibacterial activity of essential oil and methanol extract of Ziziphora tenuior on some pathogenic bacteria isolated from urogenital tract infections.

         Methods: The essential oil and methanol extract of Z. tenuior were obtained by Clevenger and maceration methods. Under sterile conditions, the required amount of urine was taken from patients referred to a number of clinics in Tabriz during 2014. After identification of the isolates using standard microbiological methods, antimicrobial effects of the essential oil and methanol extract of the plant on the isolates were evaluated by determining minimum inhibitory concentration (MIC), minimum bactericidal concentration, and antibiogram test.

          Results: The MIC for essential oil of Z. tenuior was 250µg/mL for most Gram-negative bacteria except Pseudomonas aeruginosa. Moreover, the MIC for Staphylococcus aureus was 250µg/mL and 500µg/mL for other strains of Staphylococcus.

           Conclusion: Comparison of the inhibitory and bactericidal effects of the essential oil and methanol extract of Z. tenuior showed that the essential oil is able to inhibit growth of the bacteria tested even in low concentrations. Further studies are required in this regard using animal models.

         Keywords: Antibacterial Agents, Ziziphora tenuior, Plant Extracts, Urinary Tract Infection. 

Elaheh Rezaei , Pejvak Khaki , Soheila Moradi Bidhendi , Mojtaba Noofeli ,
Volume 13, Issue 1 (Jan-Feb 2019)

            Background and Objectives: Leptospirosis is a widespread zoonotic disease that is transmitted directly or indirectly from animals to humans. Humans mainly acquire pathogenic leptospires through mucosal or percutaneous exposure to environment contaminated with urine from an infected animal. We aimed to identify pathogenic leptospiral serovars by detection of the ompL37 gene using polymerase chain reaction (PCR).
            Methods: Sixteen pathogenic leptospiral serovars and a saprophytic serovar, L. biflexa were cultured in modified semisolid Ellinghausen-McCullough-Johnson-Harris medium containing 5% rabbit serum. Genomic DNA extraction was done using the phenol-chlorophorm method. The ompL37 gene was amplified using specific primers. PCR products were analyzed by agarose gel electrophoresis.
            Results: The ompL37 gene was amplified only in the pathogenic leptospiral serovars. We detected no amplified fragment for the saprophytic serovar.
Conclusion: Leptospirosis may be confused with other infectious diseases, and therefore, its early and accurate diagnosis is crucial. We showed that molecular detection of pathogenic leptospires based on the ompL37 gene could be used for laboratory diagnosis of leptospirosis.
            Keywords: Leptospirosis, PCR, ompl37 Gene, Pathogenic Leptospires.

Page 1 from 1     

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.