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Showing 12 results for Kazemi

R Golsha, Hr Bazrafshan, V Kazemi Nejad, Mr Rabie,
Volume 3, Issue 1 (Spring - Summer 2009[PERSIAN] 2009)
Abstract

Abstract Background and objectives: In diabetic patients, infectious disease is one of the most important cause of admission, and sometimes a life threatening condition. The aim of this study was to determine the clinical features of infection in the hospitalized diabetic patients. Material and Methods: In This retrospective study, we evaluated the files of 122 diabetic patients aged over 20 years in 2004-2005. According to medical document coding, the data was collected by a checklist and then descriptive statistical analysis performed. Results: The subjects’ age is 57+11.76 years (23-83). The Mean time of diabetes diagnosis is 12.62+9.27 years. Sixty-six of them are male (54.5%).and the rest female. most of them (62.2%) were used oral hypoglycemic drugs on admission. Their median fasting blood sugar was 224mg/dl and 164mg/dl for the first and second measuring, respectively. The etiologies are soft tissue infection and diabetic foot ( 49.3%), sepsis(36%),pneumonia(5.7%),urinary infection(3.2%) and unknown(5.7%). None of diabetic foot patients was given specific education for taking care of their feet. Conclusion: in sixty -five percent of the subjects, fasting blood sugar was not in normal limit. Soft tissue infection and diabetic foot was the most infectious cause of admission. It seems that frequent control of blood sugar and foot- care related education can prevent from the most common cause of hospital admission in these patients. Key words: Diabetes, Clinical manifestations, Infection
A Moradi, E Mobasheri, A Tabarraei, S Bakhshandeh Nosrat, V Kazemi Nejad, R Azarhosh, Sh Alizadeh, M Bazori,
Volume 3, Issue 1 (Spring - Summer 2009[PERSIAN] 2009)
Abstract

Abstract Background and objectives: Breast cancer is the most prevalent one in women. Some of the common causative factors are genetic background, nutritional and environmental factors. Viruses are believed as a risk factor in this cancer, too. Recent studies reported that Human Papillomaviruses can be one of the possible risk factors of breast cancer. This study focused on investigation of the papillomavirus genome in tissues of breast cancer in Golestan province, Iran. Material and Methods: This descriptive analytical study was done from 2005 until 2008. The Samples were obtained from women admitted to the hospitals in Gorgan and Gonbad cities. All breast biopsy or mastectomy tissues were confirmed by the pathologists for breast cancer. DNA was extracted and PCR done by using general primers (GP5 + / GP6 + and MY09/MY11) for detection of papillomavirus genomes. Results: The Subjects are 231 patients aged 47± 12/72, the youngest 20 and the oldest 84. They are from Gorgan (N=122)and Gonbad (N=109) The result Shows That The Subjects Suffer from infiltrating ductular Carcinoma(31.4%), infiltrating duct Carcinoma (60.1%)and intraductal Lobular Carcinoma (4.3%) and The rest from other kinds of Cancer. Papilloma Virus genome is not found in These Samples. Conclusion: Based on paradoxical results from different parts of the world, upon the presence or absence of papillomavirus genome in breast cancer samples, to show the role of this virus in the development of breast cancer more studies are needed. Key words: Breast Cancer, Papillomaviruses, Golestan Province
F Safarnezhad Tameshkel, Mr Khatami Nejad, A Nasrollahi, P Rahdari, F Gholam Hossein Poor, S Kazemi Afarmejani,, A Rahnavard,
Volume 6, Issue 2 (Autumn- Winter [PERSIAN] 2012)
Abstract

Abstract Background and objectives: Giardiasis is a parasitic infection of small intestine, with a worldwide distribution and the prevalence of Giardia in different parts of the world varies between 1 to 25%. Plants have the vast range of antimicrobial and antifungal activity that can be identified as alternative treatments for bacterial and parasitic pathogens, the same as Giardia. In this study, the methanol extracts of eucalyptus plants, Satureia hortensis and Heracleum glabrescens, on Giardia cysts were studied in vitro. Material and Methods: The cysts were isolated from the feces using a modified Bingham. After counting by Hemusytumetr, they were placed near by 200 mg / ml, 100 mg / ml and 10 mg / ml of the extracts prepared by DMSO for 30 and 60 minutes. Then, the number of dead and live cysts was counted under a microscope. Results: the fatality effect of the extracts in 60 minutes is higher than those of 30 minutes. The methanol extracts of Satureia hortensis, Eucalyptus and Heracleum glabrescens with the dilution of 200 mg/ml in 60 mins have the fatality effect of 84/3%, 63/3% and 44%, respectively. The highest fatality(84.3%) on Giardia cysts is related to Satureia hortensis with the dilution of 200 mg/ml in 60 mins and the Lowest(27%) is related to Heracleum glabrescens with the dilution of 10 mg/ml in 30-minute period. The significant relationship between the plant type and the fatality of methanol extracts is observed. Conclusion: the methanol extracts of Eucalyptus, Heracleum glabrescens and especially Satureia hortensis have anti-parasitic effects in the laboratory conditions. Thus, they can be used in the future, instead of the chemical antiparasitic drugs. Key words: Antibacterial Giardia lamblia cysts, Eucalyptus, Satureia hortensis, Heracleum glabrescens, Tonekabon


V Kazeminejad, R Azarhoosh,
Volume 7, Issue 4 (winter[PERSIAN] 2014)
Abstract

Abstract Background and Objective: Identification of malignant cells and the type of malignancy in Effusionsis very important. The main aim of this study was to differentiate between reactive mesothelial cells and malignant cells and to determine the type of the tumor cells in effusions with the aid of tumor markers Creatine Kinase (CK), EMA and CEA. Material and Methods: Forty serous fluid cytology samples delivered to pathology laboratory of Panje- Azar Hospital (15 were malignant and 25 were suspected for malignancy) were stained by immunocytochemistry technique with the aid of aforementioned tumor markers, CK, EMA and CEA. Results: Of 15 malignancy cases, 13 were positive for three markers and the rest were negative just for CEA. In 25 of suspected to malignancy for EMA: 15 were strongly and 6 weakly positive and 4 were negative for CK : 10 were strongly and 5 weakly positive and 5 cases were negative and for CEA : 5 were strongly and 5 weakly positive and 15 were negative. Conclusion: Totally, % 87.5 of malignant fluid were positive for CK marker and %90 for EMA marker. EMA and CK were found to be the most reliable epithelial markers and very useful in differentiating carcinoma cells from reactive mesithelial cells. In Ten (40%) of the samples suspected to adenocarcinoma, CEA was positive and this indicate that CEA can be an important reference for identifying malignant effusions. Keywords: Monoclonal Antibody Cytokeratin Epithelial Memberane Antigen Carcinoemberyonic Antigen
R Golsha, V Kazeminejad, A Barzegari, S Besharat, F Ghasemi Kebria,
Volume 7, Issue 5 (supplement Issue( Bacteriology)[PERSIAN] 2014)
Abstract

Abstract Background and Objective: The excessive use of broad-spectrum antibiotics will lead to drug resistance of microorganism and specially nosocomial organisms. Because of high incidence of antibiotic resistance in hospitals, we aimed to study antibiotic resistance to gram negative bacteria. Material and Methods: This cross-sectional study was conducted on the data of biological samples (2006-2008), with positive culture result. Using antibiogram, microbial resistance to isolated microorganism was determined, and after culturing the samples, bacteria were identified by using differential media and antiserum. Then, antibiotic resistance was performed by disk diffusion. Results: The most common gram-negative microorganism obtained from all cultures was Ecoli with the lowest drug resistance to Nitrofurantoin. Conclusion: Based on the results, antimicrobial resistance pattern is not the same in different places and furthermore it is ever changing. Therefore, further research is needed to be done to have an accurate pattern of antibiotic resistance to provide effective treatment regimens. Key words: Antibiotic Resistance Disk Diffusion Gram Negative Bacteria Gorgan
A Kazemi, K Esmaili Torkanpouri,
Volume 8, Issue 3 (Autumn[PERSIAN] 2014)
Abstract

Abstract Background and Objective: Nowadays, effective human resources management plays an important role in success of organizations. In order to achieve high efficiency of limited resources, managers of organizations try to allocate the best possible human resources for every section. This kind of decision is one of the challenges that human resource managers are facing with. Material and Methods: Using analytic hierarchy process and considering qualitative and quantitative criteria, we carried out the most suitable allocation in a medical laboratory and selected the best possible team of experts for each section. Results: Regarding specific attributes eight experts were allocated to three sections of a medical laboratory. Conclusion: Analytic hierarchy process is a suitable method for allocating human resources to different sections of a company. Keywords: Human Resources, Analytic Hierarchy Process (AHP), Medical Laboratory
Saadat, S, Solhjoo, K, Norouz-Nejad, Mj, Kazemi, A, Erfanian, S, Ashrafian, F,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract

Abstract Background and Objective: Vancomycin is used for treatment of methicillin-resistant S. Aureus (MRSA) infections therefore, resistance to this antibiotic is increasing. We aimed to determine the antibiotic resistance pattern and frequency of vancomycin resistant S. Areas (VRSA) strains isolated from clinical samples. Material and Methods: In this cross-sectional study, 100 S. Aureus isolates collected from hospitals in Shiraz during six months, 2012, were identified by biochemical, microbiological and molecular methods. After determination of antibiotic susceptibility pattern by disc diffusion method and vancomycin agar screening test, Minimum Inhibitory Concentration (MIC) was determined by E-test for vancomycin, thicoplanin, linezolid and quinupristin-dalfopristin. Results: The most resistant and the most sensitive antibiotic were ampicillin (%95) and quinupristin-dalfopristin (99%), respectively, and 44% of isolates were resistant to methicillin. In agar screening test, 48% of strains had reduced sensitivity and in disc diffusion 3% strains were resistant to vancomycin. In E-test method, only one isolate was resistant to vancomycin. Conclusion: given the presence of VRSA and new antibiotic resistant strains, we recommend doing some intervention to prevent from spreading these strains in hospitals. . Keywords: Clinical Specimens, Staphylococcus Aureus, Vancomycin, Antibiotic Resistance
Zare, Z, Solhjoo, K, Norooznejad, Mj, Kazemi, A,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract

Abstract Background and Objective: Increasing prevalence of methicillin resistant Staphylococcus aureus strains (MRSA) with their multidrug resistance potential causes difficulties in the treatment of infections due to these bacteria. Hence, the detection and determination of the frequency of MRSA strains via phenotypical and molecular methods is necessary in different parts of the county. Material and Methods: In this cross- sectional study, 150 Staphylococcus aureus strains were collected from different clinical samples in the hospitals located in Shiraz and Jahrom, Iran. To detect methicillin resistant Staphylococcus aureus strains, we used phenotypical methods such as disc diffusion and minimum inhibitory concentration by E-Test, and PCR molecular method for mass gene. Results: The frequency of methicillin resistant Staphylococcus aureus was 63 strains (42%) using disc diffusion and E-Test. while in PCR method, in addition to 63 strains, nine other isolates, which were sensitive to oxacillin by disc diffusion and E-Test, possessed also mecA gene. By and large, 72 isolates (48%) had methicillin resistance gene. Conclusion: Given the results of phenotypical and molecular methods, the frequency of methicillin resistant Staphylococcus aureus was relatively high in this area. Thus, the MRSA strains can be detectable as soon as possible by accurate and sensitive methods such as PCR to determinate the effective antibiotics. Keywords: Methicillin Resistant Staphylococcus Aureus, MRSA, MecA Gene, PCR
Mohammad Mohammaddoost Chakoosari, Mohammad Faezi Ghasemi, Alireza Masiha, Reza Kazemi Darsanaki, Abolfazl Amini,
Volume 9, Issue 5 (Nov,Dec-2015 2015)
Abstract

Abstract

      Background and Objective: Probiotics are living microorganisms that have beneficial effects on the health of digestive system. The aim of this study was to evaluate the antimicrobial ability of acidic and neutral supernatants (culture supernatant) of lactic acid bacteria against common bacterial pathogens.

       Methods: Four species of lactic acid bacteria (Lactobacillus plantarum PTCC1745, Lactobacillus PTCC1608, Lactobacillus Saki PTCC1712 and Lactobacillus Lactis PTCC1336) were obtained from the microbial collection of Iranian Research Organization for Science and Technology in Lyophilized form. The antimicrobial activity of neutral and acidic supernatants against bacterial pathogens was investigated using the Disk and Well Diffusion Agar methods.

      Results: Lactic acid bacteria showed good antimicrobial ability against six pathogenic bacteria with the highest inhibitory effect observed in Lactococcus lactis against E. coli PTCC1399 through well method with an average diameter of 14 mm inhibition zone. In this study, the well diffusion method was far more sensitive compared to the disk method and acidic supernatants showed higher antimicrobial efficiency compared to neutral types.

      Conclusion:  the Metabolites produced by lactic acid bacteria are able to inhibit the growth of pathogenic bacteria that can be an important and practical solution for the prevention and treatment of infections and ultimately improve human health.


Khatoon Heydari, Ramin Azarhoosh, Vahideh Kazeminejhad, Fatemeh Shakeri, Alireza Noroozi,
Volume 9, Issue 5 (Nov,Dec-2015 2015)
Abstract

Abstract

      Background and Objective: BabA2 and Hpa genes are involved in adherence of Helicobacter pylori (H.pylori) to gastric mucosal tissue. This study aimed to investigate the frequency of these genes in isolates of H. pylori from gastric biopsies and their relationship with gastritis, peptic ulcer and gastric cancer.

      Methods: Gastric biopsy samples were obtained from patients with gastritis, peptic ulcer and gastric cancer. A sample was sent to the laboratory for urease test and histopathology study, and another sample for DNA extraction. The frequency of BabA2 and Hpa genes was investigated using their specific primers by PCR.

      Results: Among the 80 analyzed biopsy samples, 51 (63%) were BabA2 positive, and the frequency of this gene in the samples of gastric cancer, gastritis and peptic ulcer was 61.1, 58.3 and 73.3%, respectively. In addition, 57 samples (71%) were Hpa positive, and the frequency of this gene in the samples of gastric cancer, gastritis and peptic ulcer was 55.5, 69.4 and 84.6%, respectively. There was no significant correlation between the presence of these genes and the type of H.pylori-related diseases.

       Conclusion: Frequency of BabA2 and Hpa genes is higher in the samples of peptic ulcer but there was no significant relationship between these genes and H.pylori-related diseases.

      Keywords: BabA2, Hpa, Gastric Cancer, Gastritis, Peptic Ulcer.


Samira Okhli , Shaghayegh Anvari, Aylar Jamali, Mohammad Javadkazemi,
Volume 10, Issue 2 (Mar,Apr2016 2016)
Abstract

ABSTRACT

      Background and Objective: Chlamydia trachomatis is one of the most common causes of genital infection in men and women. Genital chlamydial infections in women are clinically asymptomatic in 70-80% of the cases; therefore, the lack of timely diagnosis and treatment leads to complications such as infertility and ectopic pregnancy. The aim of this study was to evaluate the frequency of chlamydial infection in symptomatic and asymptomatic women in the Golestan province.

      Methods: This cross-sectional study was conducted on 150 cervical swab samples obtained from 150 women referred to the clinic, after obtaining written consent and completion of questionnaires. The swab samples were transferred to laboratory in phosphate-buffered saline solution and DNA extraction was carried out using phenol-chloroform and boiling methods. The frequency of chlamydial infection was evaluated by PCR.

       Results: None of the tested samples were found as Chlamydia-positive.

      Conclusion: The findings require that some more extensive research with larger sample sizes and dispersed population be performed to determine the true prevalence.  Considering the serious complications of chlamydial infections and its asymptomatic nature, a highly sensitive and specific method such as PCR should be used to detect Chlamydia. It is suggested that this method be used along with a complementary test to obtain the results that are more accurate. Furthermore, conducting simultaneous studies on other populations at risk will be very helpful in obtaining representable national data.

 


Shima Kazemi , Monir Doudi , Gholm Reza Amiri ,
Volume 11, Issue 6 (Nov - Dec 2017)
Abstract

ABSTRACT
           Background and Objectives: Development of ecofriendly processes for the synthesis of metal nanoparticles is of great importance in the field of nanotechnology. Microorganisms such as bacteria could be suitable candidates for bioproduction of nanoparticles due to their simplicity and high compatibility with the environment. The aim of this study was to use bacteria isolates from the effluent of wastewater treatment plants to produce silver nanoparticles.
         Methods: For identifying silver-resistant microorganisms, we used the agar diffusion method using PHG II medium containing 0.5 mM silver to determine minimum inhibitory concentration. Bacterial identification was done with biochemical testing and polymerase chain reaction (colony PCR). Finally, silver nanoparticles were produced in the desired bacteria, and the properties of these nanoparticles were studied.
         Results: We found five silver-resistant bacteria among which Stenotrophomonas maltophilia strain MS8 showed the highest resistance (MIC= 6 mM). The bacterium was able to synthesize silver nanoparticles in spherical shapes. The results obtained from visual observations using UV-VIS, TEM and XRD showed that the bacterium was able to reduce silver ions into silver nanoparticles with maximum size of 20 nm.
Conclusion: Based on our findings, this bacterium could be useful for biosynthesis of silver nanoparticles.
          KEYWORDS: Bacteria, Biosynthesis, Minimum Inhibitory Concentration. 


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