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Showing 2 results for Hakimi Alni

Azizollah Ebrahimi Kahrizsangi , Saied Habibian Dehkordi , Ziba Shabanpur, Reza Hakimi Alni , Majid Hemati,
Volume 10, Issue 6 (Nov-Dec-2016 2016)


         Background and Objective: Biofilms are community of bacteria that attach to inanimate surfaces or living tissues via production of extracellular polymers and exopolysaccharide matrix. Microbial biofilms on various surfaces of the hospital environment are considered as a reservoir of infection spread. The present study aimed to evaluate the disinfecting effect of benzalkonium chloride on some bacterial isolates causing nosocomial infections.

       Methods: First, 13 isolates from four bacteria including Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter and Enterobacter were obtained from Microbiology Laboratory of Al-Zahra Hospital in Isfahan, Iran. The samples were transferred to Microbiology Laboratory of Faculty of Veterinary Medicine of Shahrekord University for testing. Evaluation of biofilm formation and determination of minimum inhibitory concentration (MIC) of the disinfectant and effect of the disinfectant on planktonic growth and biofilm formation were performed.

        Results: All bacterial isolates (52 cases) produced biofilm. Mean MIC of benzalkonium chloride for P. aeruginosa, S. aureus, Enterobacter and Acinetobacter was 0.14, 0.2, 0.18, 0.17 g/ml, respectively. Planktonic growth of all four bacteria was inhibited at concentrations of 2MIC, MIC and 1/2MIC. Biofilm was not produced in MIC and 2MIC concentrations, and biofilm formation capability increased by reducing the concentration of benzalkonium chloride.

          Conclusion: The results show that the use of appropriate concentration of benzalkonium chloride can prevent the growth of different bacterial species, but sub-MIC dose of this disinfectant may stimulate biofilm formation.

            Keywords: Biofilm, Benzalkonium Chloride, Pseudomonas Aeruginosa, Staphylococcus Aureus, Enterobacter, Acinetobacter.

Reza Hakimi Alni , Abdolmajid Mohammadzadeh , Pezhman Mahmoodi , Mohammad Yousef Alikhani ,
Volume 11, Issue 6 (Nov - Dec 2017)

          Background and Objectives: Determining the genetic relationship between S. aureus isolates is important for epidemiological surveillance and control of infections caused by this bacterium. The present study was conducted to determine polymorphisms of coagulase gene (coa) among S. aureus isolates from pastry and cheese samples using restriction fragment length polymorphism (RFLP) analysis.
         Methods: Overall, 65 S. aureus isolated from pastry (n=45) and cheese (n=20) samples were examined for the coa gene by polymerase chain reaction (PCR). PCR products were digested with AluI enzyme and the products were assessed using gel electrophoresis.
          Results: Except for two isolates, all isolates were positive in coa-PCR and produced four different PCR products, with molecular sizes ranging from 570 to 970 bp. Overall; five distinct RFLP patterns were detected (I-V). Although pattern types I and III were present in isolates from both samples, types I and IV were mainly present in isolates from cheese and pastry samples, respectively.
        Conclusion: PCR-RFLP analysis of the coa gene indicates that S. aureus isolates from pastry and cheese samples may be originated from different sources. However, as one pattern type was predominant in each group, it can be concluded that majority of the isolates may have the same origin.
          Keywords: Staphylococcus aureus, PCR-RFLP, Coagulase, Pastry, Cheese.

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