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Showing 5 results for Habibipour
Antibacterial Effects of Extract of Alloe Vera and Stachys Inflata on Gram Positive and Negative Bacteria in In Vitro
M Rajabi, R Habibipour, S Vesaghati Ezatpour, S Vesaghati Ezatpour,
Volume 8, Issue 3 (Autumn[PERSIAN] 2014)
Abstract
Abstract Background and objective: Antimicrobial and antiviral effects of Alloe Vera and Stachys inflata have been proved. We aimed to investigate the effects of extract of Alloe Vera and Stachys inflate on the growth of some bacteria to take the place of chemical drugs. Material and Methods: the extracts of both plants were prepared by maceration method different concentrations were prepared using Mueller Hinton agar medium and tested by Disc diffusion. Furthermore, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the Microdilution method. Results: The effect of Alloe Vera extract was significant on Staphylococcus aureus. MIC and MBC of Aloe Vera extract on Bacillus subtilis were obtained in 230 and 410 mg/ml, respectively, which were 500 and 714 mg/ml for Haemophilus influenza. The extract of gel of Alloe Vera had no effect on Bacillus subtilis. The extract of leaf and gel of Alloe Vera had an inhibitory effect on Haemophilus influenza and Pseudomonads aeraginosa. The extract of Stachys inflata had an inhibitory effect on Haemophilus influenza, but it did not have any on Pseudomonads aeraginosa. The Extract of Stachys inflata had no effect on Bacillus subtilis, while showing significant effect on Staphylococcus. Among antibiotics, Ofloxacin had an effect on Haemophilus influenza. The extract of both plants did not show any effect on Klebsiella pneumonia. Conclusion: Given the effect of Alloe Vera and Stachys inflata in laboratory conditions, we hope that these extracts will be used instead of chemical substances for making nutritional supplements to control human diseases. Key words: Alloe Vera, Antibacterial, Stachys Inflata, Extract
Comparison of clinical and paraclinical diagnosis of vulvovaginal candidiasis in females referred to hospitals in Hamedan city
Habibipour, R,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract
Abstract
 
Background and Objective: Vulvovaginal candidiasis (VVC) often refers to yeast infection, which is a common gynecologic problem, affecting 75% of women during their lifetimes. This study was conducted to evaluate clinical and paraclinical diagnosis of VVC in females.
 
Material and Methods: In this cross-sectional study, Vulvovaginal samples were collected from 350 women suspected vulvovaginitis verified by gynecologist. After checking microscopic fungal elements, samples were cultured on Chrom Candida Agar and Sabourraud Dextrose Agar. Using a checklist, their personal, social and hygienic status were  evaluated.
 
Results: The rate of infection was 21.1%   with wet mount , 21.7% with staining and 26% with culture procedure. Physicians' diagnoses were not  in line with para-clinical experiments in 80.2 percent (P<0.05).
 
Conclusion: based on the results , vulvovaginal candidiasis can only be diagnosed by using clinical criteria related to vulvovaginal symptoms and Candida cultures. This matter can be helpful to prevent and reduce the side effects of some unnecessary medications.
Keywords: Candida Albicans, Clinical Experiments, Vaginitis, Para- Clinical Experiments
 

Effect of Silver Nano-Particle on Removing the Enterococcus faecalis Bacterium isolated from Industrial Resid
Sadeghian, M, Habibipour, R, Asghar Seif,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract

Abstract

Background and Objectives: Due to the unique absorption and antibacterial properties of nanoparticles, their use in water and wastewater treatment to remove bacteria is spreading. This research aimed to study the effect of silver nanoparticles in the removal of Enterococcus faecalis isolated from industrial sewage.                                               

Material and Methods:  after preliminary studies, field studies including sampling of industrial wastewater was conducted.  First, Fecal Coliform, total coliform and Enterococcus faecalis of industrial Sewage were measured by standard methods of microbiology, then the effect of concentrations of 500, 250, 125, 62.5, 31.25, 15.62, 7.81, and 3. 9 PPM of isolated nanoparticles serial dilution method on Enterococcus faecalis and standard (PTCC 1339) was determined. Next,  and the turbidity of tube (OD), which represents growth of bacteria, was read at 600 nm with a spectrophotometer. After that,  all tubes were cultured on solid medium and the results were analyzed using SPSS 22.                                                                                                            

Results: fecal and total coliform contamination of industrial Sewage from slaughter and textile plant were approved and Enterococcus faecalis was isolated. The growth of isolates and standard bacteria was not controlled by any of these concentrations. None of the concentrations was able to remove the bacteria but the increase of concentration led to higher effect of nanoparticles, and in accordance with the type of bacteria, it was different, but the difference was not statistically significant.                                                                                                         

Conclusion: Although silver nanoparticles ,according to the concentration,  affect the Streptococcus faecalis, they cannot  completely remove bacteria. Therefore,  the economic factors and other involved factors need to be considered in the application of nanoparticles.                                                                                                           

Keyword: Silver nanoparticles, Enterococcus faecalis, industrial Sewage


Isolation and Characterization of Hyperthermophilic Nanobacteria from a Hot Spring in Ardabil, Iran
Reza Habibipour, Gholamreza Zarrini, Shohreh Yarizadeh,
Volume 11, Issue 3 (May-Jun 2017)
Abstract
ABSTRACT
       Background and Objective: Nanobacteria are nanometer-scale particles with different shapes, which have been a subject of debate in modern microbiology. They belong to a proposed class of living organisms, specifically cell-walled microorganisms with a size much smaller than the generally accepted lower limit for life. Since some microorganisms are able to continue growth at high temperatures, we aimed to isolate thermophilic bacteria from Gheinarcheh hot spring in Ardabil (Iran) and identify the characteristics of these microorganisms.
       Methods: Microbial mats were found in cultures from Gheinarcheh hot spring in North West of Iran. Synthetic media were prepared and used for isolation of protease-producing thermophilic bacteria, and identification of the features of microbial mats. Cultures were incubated at temperature range of 60-120 oC. Scanning electron microscopy, DNA extraction and polymerase chain reaction were used to further identify characteristics of the microbial biofilms.
        Results: Microbial biofilms of nanoparticles were detected in our samples. Growth of the bacteria increased at all temperatures tested. Results of scanning electron microscopy showed nano-sized particles in the scale of 60 nm. No band was visible in gel electrophoresis of polymerase chain reaction products.
Conclusion: This study is the first to report the presence of hyperthermophilic nanobacteria in Iran.
      Keywords: Nanobacteria, Hyperthermophile, Microbial Mat.

Investigating Effects of Superparamagnetic Iron Oxide Nanoparticles on Candida albicans Biofilm Formation
Fateme Golipour, Reza Habibipour, Leila Moradihaghgou,
Volume 13, Issue 6 (Nov-Dec 2019)
Abstract
ABSTRACT
           Background and Objectives: Candida albicans is one of the most common fungal pathogens that can form biofilm, particularly on surface of medical devices. In recent years, C. albicans has shown increased resistance to antifungal agents. In this experimental study, we aimed to study effects of superparamagnetic iron oxide nanoparticles (Fe3O4 nanoparticles or SPION) on biofilm formation by C. albicans.
           Methods: First, the SPION were synthesized by chemical co-precipitation. The formation of nanoparticles was confirmed by Fourier-transform infrared spectroscopy and X-ray diffraction. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of SPION were determined. Then, antibiofilm effects of the nanoparticles were investigated by enzyme-linked immunosorbent assay. Finally, data were analyzed using SPSS 22.0 at significance level of 0.05.
           Results: According to the results of X-ray diffraction, the SPION had a mean diameter of about 70 nm. MIC and MFC values of SPION against C. albicans were 100 ppm and 200 ppm which reduced biofilm formation by 87.2% and 100%, respectively. SPION showed significant inhibitory effects on C. albicans growth and biofilm formation.
           Conclusion: Based on the findings, SPION may be considered as a novel family of fungicidal compounds. However, further studies are necessary to evaluate the safety of these nanoparticles for treatment of fungal infections in humans.
           Keywords: Candida albicans; Biofilms; SPION; Nanoparticles.

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