Showing 9 results for Fallah
H Taherkhani, Kh Jadidian, M Fallah, S Vaziri,
Volume 1, Issue 2 (Autumn – Winter 2008[PERSIAN] 2007)
Abstract
Abstract Background and objectives: Acquired immunodeficiency syndrome (AIDS) is acquired by infection with human immunodeficiency virus. (HIV).Parasite caused infection is the most common problem in AIDS Patients. Sometimes unusual and opportunistic infections such as intestinal parasitic infections may cause serious gastric intestinal (GI) disorders, which can finally lead to death. The aim of this study is to find intestinal parasites in AIDS patients referred to Disease Consultation Center of Kermanshah province. Materials and methods: In this study, the stool samples were collected from 75 AIDS patients admitted to Kermanshah consultation disease center. Then, all samples were tested by direct and formalin ether methods. Results: As shown in results , 13 patients (17.4%) are infected with Entamoeba coli, two( 2.7%) with Entamoeba histiolyticaly,one(1.4%) with Giardia lumbelia ,six (8%) with Blastocystis hominis ,two( 2.7%) with Isospora belli ,one(1.4%) with Ascaris lumbericoides ,two( 2.7%) with Endolimax nana . Conclusion: This study indicated that the frequency of pathogenic and non-pathogenic parasites was low in HIV+ patients. This may be related to anti-parasitic drugs used in all HIV+ patients admitted to Kermanshsh nsultation Disease center. Key words : Intestinal parasitic Infections, AIDS, Kermanshah consultation Disease center
Z Gharibi, F Daadras, M Maghsood, M Fallah,, M Saeedijam,
Volume 7, Issue 4 (winter[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: Microsporidia is an obligatory intracellular parasite known as an opportunistic infection in immunocompromised patients. Its laboratory diagnosis is relatively difficult and modified trichrome staining is a standard diagnostic method for detection of Microsporidia. The aim of present study was to identify intestinal Microsporidia in kidney- transplanted patients, using modified Trichrome Staining and Calcofluor White Methods.
Material and Methods: In 2012, 180 stool specimens were taken from kidney transplanted- patients given immunosuppressive drugs in Hamadan, Iran. To identify Microsporidia we use modified Trichrome Staining and Calcofluor White Methods.
Results: The mean duration of kidney transplant and immunosuppressive drug using was 5.5 Years. Only one female patient was positive for Microsporidia.
Conclusions: Owing to low frequency of this opportunistic infection among kidney transplanted patients, we can conclude that their hygienic conditions are good enough and they are not exposed with the parasites.
Key words:Microsporidia, Modified Trichrome Staining,Kidney Transplant, Calcofluor White, Hamadan
Derakhshan, S, Najar Peerayeh, Sh, Fallah, F, Bakhshi, B,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Multiple drug resistance has increased in recent years in Klebsiella pneumoniae isolates. The Integrons are mobile genetic elements that carry antibiotics resistance genes. The aim of this study was to determine antibiotic susceptibility and the prevalence of class 1, 2, and 3 integrons in clinical Klebsiella pneumoniae isolated from clinical specimens. Material and Methods: A total of 108 K. pneumoniae isolates were collected between April and December 2011 from different clinical specimens of Loghman hospital in Tehran and identified by biochemical tests. Susceptibility of isolates to 14 antibiotic disks was determined by disk diffusion method. The template DNA was extracted by freeze-thaw method and the presence of class 1, 2, and 3 integrons was investigated by PCR method. Level of resistance to antibiotics in integron-positive and integron-negative isolates was determined. Results: The highest level of resistance was seen for cefotaxime, ceftriaxone, and amoxicillin-clavulanic acid (55.5%). In 79 isolates (73.14%) class 1 integron and in 57 of 79 isolates (72.15%) resistance to at least two classes of drugs were seen. The class 2 and 3 integrons were not detected. Among integron-negative isolates, 8 isolates (27.58%) had resistance to at least one antibiotic. Conclusion: The prevalence of class 1 integron in resistant K. pneumoniae is high therefore, the monitoring of drug resistance and limiting the use of antibiotics are necessary. Keywords: Klebsiella Pneumoniae, Integron, Multi-Drug Resistance
Mahboobi, R, Fallah Mehrabadi, J, , Pourmand, Mr, Mashhadi, R, Haddadi, A,
Volume 8, Issue 5 (winter[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Increased antibiotic resistant strains and inadequacy of current vaccines against pneumococcal infections necessitate the study of novel protein antigens. It seems that minor autolysin of Streptococcus pneumoniae may have antigenicity. Thus, we aimed at cloning its gene for the first time. Material and Methods: After DNA extraction of Streptococcus pneumoniae (ATCC 49619), Specific primers were designed for amplifying minor autolysin gene fragment, using PCR. The purified gene fragment was inserted into pET21a vector and was transformed into bacterial competent cells by heat shock technique. The presence of gene and absence of mutation in the recombinant vector were checked out with sequencing and enzymatic digestion methods. The gene sequence was finally analyzed by bioinformatic tools. Results: The gene of minor autolysin was cloned successfully and the result of enzymatic digestion was the indication of complete isolation of this gen from plasmid. . Bioinformatics studies revealed that the mature protein was lacking signal peptide and the gene encoded 318 amino acids with a molecular weight of 36.4 kDa. Conclusion: The presentation and characterization of novel antigens such as minor autolysin could help us with finding new approaches for preventing and controlling pneumococcal infection. Keywords: Streptococcus Pneumoniae, Minor Autolysin, Cloning
Moradi, M, Matini, M, Mohemmi, N, Maghsoud, A, Zahirnia, A, Parsa, F, Fallah, M,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract
Background and Objective: Assessment of alimentary tract helminthes of rodents has a great zoonotic importance. This study aimed at determining the prevalence of helminth infections in rodents in Hamadan.
Material and Methods: a total of 100 rodents trapped from different parts of city were transported to laboratory. After anesthetizing by chloroform, the animals were undergone an operation to isolates the helminthes. The isolates were stained by Carmine and identified at the genus and species levels. Furthermore, age, sex, weight of rodent and size of various organs of body were determined.
Results: Totally, 62% of the rodents were infected to intestinal helminthes. All trapped rodents were Rattus norvegicus. Six species of helminthes, including three Nematode (45%), 3 Cestode (51%) and no Trematode were isolated from rodents. The infection rate for different helminthes was as follow: Hymenolepis nana 21%, H. diminuta 29%, Heterakis spomosa 43%, Strongyloides sp. 1% Trichuris muris 1% and Cysticercus fasciolaris 1%.
Conclusion: in this area, infection rate of alimentary tract helminthes in the Rattus norvegicus, especially zoonotic helminthes, is relatively high, and the rate of Cestodes is higher than those of Nematodes and Trematodes.
Key words: Prevalence, Helminthes, Alimentary tract, Rodents
Hesam Alizade , Fatemeh Fallah , Reza Ghanbarpour , Hosein Goudarzi , Hamid Sharifi , Mohammad Reza Aflatoonian ,
Volume 10, Issue 2 (Mar,Apr2016 2016)
Abstract
ABSTRACT
Background and Objective: One of the main tasks of clinical microbiology laboratories is to determine antibiotic resistance profiles in common pathogens and ensure the selection of effective antibiotics for certain infections. The aim of this study was to compare the methods of disk diffusion, broth microdilution and modified Hodge test in Escherichia coli isolates from urinary tract infection and diarrhea for susceptibility testing against beta-lactam antibiotics in Kerman, Iran.
Methods: In this study, 432 E. coli isolates were collected from diarrhea (216 isolates) and urinary tract infection samples (216 isolates). The antibiotic susceptibility testing methods of disk diffusion, broth microdilution and modified Hodge test were performed according to the Clinical and Laboratory Standards Institute guidelines.
Results: The findings of disk diffusion method showed that resistance to cefotaxime, ceftazidime, aztreonam, cefepime and imipenem was 51.15%, 30.55%, 24.30%, 15.27% and 1.85%, respectively. In the disk diffusion test, 51.15% of the isolates were resistant to at least one antibiotic, all of which were later evaluated by the broth microdilution method. Moreover, 52.94%, 17.19%, 13.12% and 0.90% of the isolates were resistant to cefotaxime, ceftazidime, cefepime and imipenem, respectively. All of the isolates were evaluated for the production of carbapenemase enzyme by the modified Hodge test and none of the isolates were found as positive.
Conclusion: This study shows that performing carbapenem tests is very challenging, and laboratories are recommended to use secondary and independent antibiotic susceptibility tests such as modified Hodge test to confirm the carbapenem-resistant results.
Soghra Rabiee, Sima Saffari, Nazanin Fallah,
Volume 12, Issue 1 (Jan-Feb 2018)
Abstract
ABSTRACT
Background and Objectives: Human chorionic gonadotropin (hCG) is the embryonic hormone and one of the most important factors for implantation. The present study aimed to evaluate effect of intra-uterine injection of hCG before embryo transfer on the pregnancy outcome in IVF.
Methods: In this study, 180 infertile patients were randomly allocated into an experiment and a control group. In the experiment group, before embryo transfer, 500 IU hCG were injected into the endometrium cavity in puncture stage. Equal amount of phosphate buffer (placebo) was injected to the subjects in the control group. Pregnancy test was done two weeks after the intervention. In case of positive results, ultrasound scan was done for detecting the gestational sac and numbers of sacs.
Results: Mean age of the patients was 30±3.41 years and 31±4.12 years in the experimental and control group, respectively. There was no statistically significant difference in the duration of infertility between the two groups (4.77±3.67 years vs. 4.87±3.74 years). Based on the hCG level, the pregnancy rate was 35.5% in both groups. However, based on ultrasound findings, the pregnancy rate was 35.5% and 28.8% in the experiment group and the control group, respectively (P=0.33). In addition, there was no significant difference in the pregnancy rates between different age groups.
Conclusion: Our results indicate that the intra-uterine injection of β-hCG has no significant effect on the pregnancy rate in IVF.
Keywords: Fertilization in Vitro, Chorionic Gonadotropin, Injections.
Mehdi Seydyousefi , Ziya Fallahmohammadi , Mahtab Moazzami , Ali Yaghoubi , Zeinab Faghfoori ,
Volume 13, Issue 2 (Mar-Apr 2019)
Abstract
ABSTRACT
Background and objectives: Stroke is one of the leading causes of death and long-term acquired disability. It is of great importance to seek ways for improving motor, sensory, and cognitive function after stroke and brain injury. In this regard, therapeutic exercise is the most commonly used method of rehabilitation that can significantly reduce the severity of functional damage. The aim of the present study was to investigate effects of eight weeks of forced treadmill training on cognitive and motor functions in ischemic rats.
Methods: Fourteen adult male Wistar rats were divided into an exercise group and a control group (no exercise). Occlusion of both common carotid arteries was made to induce cerebral ischemia. Twenty-four hours after the induction of ischemia, the subjects in the exercise group were subjected to treadmill running, five days a week for eight weeks. The skilled ladder rung walking task was used to evaluate motor function before and after the stroke.
Results: The number of errors was decreasing in both groups, but significant differences were observed in the motor function between the two groups in the third, fifth, and eighth week.
Conclusion: Our results suggest that post-ischemic exercise might modulate ischemia-induced hippocampal injury and ameliorate the subsequent memory and motor impairment.
Keywords: Stroke, Cerebral Ischemia, Exercise, Treadmill training, sensorimotor.
Mohammad Fallah, Alireza Shiri, Amir Hossein Maghsood, Mohammad Matini,
Volume 15, Issue 3 (May-Jun 2021)
Abstract
Background and objectives: Hydatidosis is an important zoonotic disease with widespread distribution. For unknown reasons, some cysts are unable to produce protoscoleces, and little is known about the mechanisms involved in infertile cyst production. Therefore, characterization of hydatid cyst fluid (HCF) components could help clarify the host-parasite relationship and the fertility process of cyst. The aim of this study was to identify and quantify biochemical components of HCF of fertile and infertile cysts from different hosts.
Methods: A total of 35 HCF samples were obtained from the liver and lung of 16 cattle, 16 sheep and three humans. Fertility of cysts was determined by examination of cysts’ content. Then, total fluid was aspirated aseptically from each cyst. The samples were centrifuged at 10000×g for 15 min at 4°C, and then concentrated and dialyzed against phosphate buffer saline using an Amicon Ultra-15 5000 MWCO centrifugal filter device (Millipore, USA). All biochemical components were quantified by an automatic analyzer.
Results: The value of lactate dehydrogenase, potassium, calcium, cholesterol, glucose, urea and uric acid differed significantly in cysts collected from different hosts (P<0.001). There was also a significant difference in the amount of lactate dehydrogenase, aspartate aminotransferase and cholesterol between fertile and infertile cysts (P<0.039).
Conclusion: Due to the differences in biochemical composition of HCF of different host origin, it is possible that the host plays a crucial role in determining the type of biochemistry in hydatid cyst as well as in hydatid cyst fertility.
