Showing 3 results for Bokaeian
M Bokaeian, H A Khazaee, M Javadi Mehr,
Volume 4, Issue 1 (Spring - Summer 2010[PERSIAN] 2010)
Abstract
Abstract Background and objectives: Colonization of nasopharynx by Streptococcus Pneumoniae can lead to pneumococcal disease. This study was performed to determine the carriage rate of nasopharyngeal S.pneumoniae in adolescents and their antibiotic susceptibility and serotype prevalence in Zahedan, Iran Material and Methods: Nasopharyngeal specimens were obtained from 865 adolescents aged 10-19 years old of eight schools in Zahedan and then assessed by standard procedures to isolate S. Pneumoniae. The serotyping was carried out by latex agglutination test, the minimum inhibitory concentration (MIC) of penicillin, as well as other commonly used antibiotics, was determined by a broth-dilution method. Results: Pneumococci were Isolated from 15.7% [136/865, 95% confidence interval (CI) 12.3-18.9] of total samples. Of 136 samples, 119 isolates are typified by the available antisera which the most frequent ones are 1, 19A, 15C, 9V, 11A and 19F. Ninthythree pneumococcal isolates are sensitive to penicillin. The MIC values of antibiotics tested are (μg/ml): penicillin 0.01-4, cefotaxime 0.01-4, ceftriaxone 0.02-128, chloramphenicol 0.08-32, ciprofloxacin 0.06-16, erythromycin 0.01-128, tetracycline 0.08- 128 and vancomycin 0.02-1. Conclusion: A clear diversity is seen in the serotype distribution of the S. Pneumoniae isolates and most of the antibiotic resistant strains belonge to a few serotypes. Healthy adolescents in Zahedan commonly show pneumococcal carriage and antibiotic resistance. Keywords: Streptococcus Pneumoniae, nasopharyngeal carriage, penicillin resistance, serotype
Shahraki, Sh, Bokaeian, M, Rigi, Sh,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Klebsiella pneumoniae is an opportunistic nosocomial pathogen causing a variety of infections including urinary tract infections, pneumonia, septicemia, wound infections and infections in the intensive care units. Since the ESBL producing Klebsiella pneumoniae strains are increasingly causing urinary tract infections, we aim to assess antibiotic resistance pattern and evaluate the prevalence of ESBL in Klebsiella pneumoniae isolated from urinary tract infections. Material and Methods: this cross-sectional study was conducted on 122 Klebsiella pneumoniae strains collected from Zahedan hospitals. After final identification of isolates, antibiotic susceptibility tests were carried out by using disk diffusion in agar method for 16 antibiotics and ESBL production was determined by the combined disk method. Results: The Klebsiella pneumoniae strains showed susceptibility to imipenem and amikacin ( 94.3%) ,chloramphenicol (88.5%) , gentamicin (81.1%) , ciprofloxacin (80.3%) , cefepime (73%) ,streptomycin (72.1%), nalidixic acid (68%) , tetracycline (65.6%), and cefotaxime, ceftazidime, cefpodoxime (62.3%) . The resistance of strains was seen to nitrofurantoin (53.3%), cotrimoxazole (39.3%), Cefpodoxime (37.7%), cefotaxime (36.9%), ceftriaxone (36.1%), aztreonam (34.4%), ceftazidime (32.8%). Thirty-eight isolates (31.1%) were shown to produce ESBLs. Conclusion: A high rate of resistance was observed to most of the antibiotics among ESBL producing strains therefore, it is important to be careful about the use of antibiotics and identification of ESBL using phenotypic methods. Keywords: Antibiotic Resistance, Extended Spectrum Beta-Lactamases,KlebsiellaPneumoniae, Urinary Tract Infection, Isolate
Mohammad Bokaeian, Shahram Shahraki Zahedani , Abbasali Delarampoor, Mohammadreza Atashgah, Bahram Dahmarde ,
Volume 12, Issue 3 (May-Jun 2018)
Abstract
ABSTRACT
Background and Objectives: The resistance of gram-negative bacteria to antibiotics has become a serious problem, which imposes a significant increase in treatment costs. Klebsiella pneumoniae is an important nosocomial pathogen from the Enterobacteriaceae family. The aim of this study was to investigate the frequency and pattern of antibiotic resistance in K. pneumoniae strains isolated from clinical samples.
Methods: This descriptive, cross-sectional study was performed on 150 K. pneumonia strains isolated from different clinical samples such as urine, sputum, blood, ulcers, lung secretions and abdominal abscess. Antibiogram test was performed using the disk diffusion method (Kirby-Bauer). Minimum inhibitory concentration of amikacin, tobramycin and gentamicin was determined via the E-test for 50 strains with high resistance rates.
Results: In this study, the highest rate of resistance was observed against carbenicilin, ceftriaxone, cefepime and streptomycin. K. pneumonia isolates were most frequent in urine and sputum samples. In the E-test, the highest rate of resistance was observed against gentamicin, tobramycin (16µg/ml) and amikacin (64µg/ml).
Conclusion: Based on our results, tigecycline, netilmicin, kanamycin and amikacin are the most effective antibiotics for the treatment of K. pneumoniae infections.
Keywords: Klebsiella pneumoniae, antimicrobial resistance, E-test method
