Showing 12 results for Alavi
F Niknezhad, Kh Kalavi, M Mofidi, A Sarikhani,
Volume 1, Issue 1 (Spring - Summer 2007 [PERSIAN] 2007)
Abstract
Abstract Background & Objectives: Thalassemia syndromes isone of the inherited disorders in which one or more globulin chains are affected. On the basis of clinical symptoms, thalassemias are categorized as minor, intermediate, and major. Minor beta -thalassemia is a mild microcytic hypo chromic anemia in most cases asymptomatic and HbA2 is more than normal. Materials & Methods: This study carried out on 813 blood samples obtained from male high school students of Aliabad Katool, in the north of Iran. After detecting red blood cell indices of the subjects whose MCV was less than 80fl HbA study performed. Results: The MCV of 8.24% of the subjects was less than 80fl.34 of 67 had HbA2 of less than 3.5 %(normal) while the rest more than 3.5%(minor). Conclusions: Since about 50% of microcytic anemic patients were the carrier of beta-thalassemia and it is highly Prevalent in this region (4.06%), we recommend to investigate HbA2 in people with MCV less than 80 fl. Keyword: Thalassemia, Thalassemia minor, HbA2, Iran
A Alavi, Sh Moradi, N Mirkheshti, A Ghadiri, F Hadizadeh,
Volume 1, Issue 1 (Spring - Summer 2007 [PERSIAN] 2007)
Abstract
Abstract Background and objectives: Hemin is a porphyrin compound derived from hemoglobin, the precursor of other porphyrin hemoglobin derivatives and the raw material of Hematin. Since hemin is widely used in medicine, we decided primarily to synthesize this substance in Laboratory and to determine the best way of hemin extraction from untransfused and expired blood units. Materials and Methods: In the first method, Glacial acetic acid and sodium chloride were added to citrated blood and hemin crystals were extracted by means of cooling. Finally, the obtained product, by visible spectrophotometer and Infrared Spectrophotometer, was compared to standard samples. Fur thermore, citrated blood, citrated blood hemolysed by distilled water and citrated blood washed by normal saline were used comparatively as a raw material to produce Hemin. The second method was performed by adding Strontium, acetic acid and acetone to blood samples and then after precipitating Hemin crystals they were washed and dried with acetone. Results: The presence of functional groups in Hemin samples, analyzed by infrared Spectrophotometer, indicates the production of this compound. The results of visible Spectrophotometer in comparison with control samples and the results of samples weighting demonstrates high efficiency of extraction stages and the purity of obtained compound. Conclusion: The use of intact citrated blood produces more Hemin than the other kind of Citrated blood samples. Moreover, acetic acid with citrated blood, without any processing on blood, is the best way for Hemin production. Key words: strontium, Hemin, Blood, acetic acid, extraction
Kh Kalavi, A Moradi, Ar Ahmadi, Aj Sarikhani, M Bazoori, Mr Kyaee,
Volume 2, Issue 1 (Spring - Summer 2008[PERSIAN] 2008)
Abstract
Abstract Background and objectives: Human T-Lymphocyte Virus-1 (HTLV- 1) is known as the etiologic factor of acute T-Lymphocytic Leukemia (ATL) and tropical spastic paralysis. (TSP). Endemic factors causing infection with Human T Lymphocyte Virus-1 (HTLV-1) is based on environmental, socio-economical and health behaviors of the individuals. This virus is well distributed in families with involved members. Golestan province is located in North West part of Northern Khorasan province that had already been known as an endemic area for HTLV-1. This virus is also known as the main etiologic factor for cancers and ATL, therefore we studied the prevalence of HTLV-1 seroepidemiology in Golestan province. Material and Methods: The subjects selected by cluster sampling were 2034 healthy cases residing in different parts of Golestan province. ELISA method using Dia- pro anti HTLV-1 antibody kits was applied for serological assessment. Western Blot (HTLV BLOT 2.4) was used for confirmation purposes. Results: The subjects aged 38.66±16.54 were 2034 healthy persons. Forty-one point seven of these cases were males and the rest females. Based on ELISA method there were15 HTLV-1 positive cases (0.7%). -1. (0.7%) Six out of 15 were confirmed by western blot method (95%, CI: 0.06-0.53%). The highest prevalence sigllificant) aiology is in the highat rate in 31-40 year old gro0.7%). onclusion: This study shows that HTLV-1 is prevalent in Golestan the same as the other parts of the world. There fere: we urse on performing screening test (HTLV-) on donated blood components before delivering (OK labeling). Key words: HTLV-1, Seroepidemiology, ELISA, Western Blot, Golestan ATL(Acute T lymphocytic Leukemia) Six cases out of 15 were confirmed by western blot method (95%, CI: 0.06-0.53%). The highest prevalence was 2.6% seen in Kalaleh city (east part of the province) [95%, Cl: 0.06-0.53%). There was significant difference between the prevalence of HTLV-1 and the dwelling place. (p=002). HTLV-1 seroepidemiology was in the highest rate in 31-40 year old group (0.7%). Conclusion: This study shows that HTLV-1 is prevalent in Golestan province, the same as the other parts of the world. Therefore, we recommend performing screening test (HTLV-) on donated blood components before delivering (OK labeling). Key words: HTLV-1, Seroepidemiology, ELISA, Western Blot, Golestan province, ATL (Acute T lymphocytic Leukemia)
Hr Joshaghani, Kh Kalavi, N Namjoo,
Volume 2, Issue 2 (Autumn – Winter 2009[PERSIAN] 2008)
Abstract
Abstract Background and Objectives: Erythrocyte indices are helpful for monitoring the erythrocyte synthesis and liver condition. The pesticide factories’ staff are being exposed to poisoning. This article aims to evaluate the effect of pesticides on blood indices of subjects which are exposed to organophosphate toxins. Material and Methods: This cohort study was conducted on the 63 employees of a pesticide factory In Gorgan.Iran (2005). We performed a two-step Sampling, The beginning of working season and three months later. Then, data analysis was carried out (p<0.05). Results: The difference between the mean of MCV in phase one (82.8 ± 7.1 fl) and phase two (80.3 ± 6.7) is Significant (p<0.001). The increase of Erythrocyte, hemoglobin, HCT, and MCHC in the second phase was statistically meaningful. There is not any significant relation between the work place, Record of Services and red blood cell indices. Conclusion: In regard to the changes in hematologic indices in a threemonth period, it seems logical to set up a program to have a routine check on the hematologic indices in people engaging in such occupations Key words: Pesticide, organophosphate toxins, hematologic indices, MCV, hemoglobin, hematocrit.
A Hajihasani, M Douraghi, M Rahbar, M Mohammadzadeh, H Zeraati, S Ghoorchian, M Alavi Moghadam, M Sabzi,
Volume 8, Issue 2 (summer 2014[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: Stenotrphomonas maltophilia is an opportunistic nosocomial pathogen with high mortality in immunocompromised cases. The aim of this study was to isolate and identify Stenotrphomonas maltophilia in the hospitals’ environment and wards.
Material and Methods: In this cross-sectional study, a total of 1108 samples were collected from environment of two hospitals during 12 months. Identification of isolates was performed using biochemical, phenotypic (intrinsic resistance to carbapenems) and molecular methods (amplification of 23S rRNA gene).
Results: Of the studied samples, 186 (16.78%) nonfermentative gram negative bacilli (NFGNB) were identified. Amongst NFGNB, 18 (1.62%) isolates were identified as S. maltophiliaby using biochemical tests. Of 18 biochemically identified isolates, 15 (83.3%) were confirmed via PCR. Sinks (40%) and men surgery ward ( 33.3 %( were the most contaminated sites and wards of hospitals, respectively.
Conclusion: S. maltophilia is repeatedly isolated from sink which shows that the moist hospital environments need to be considered as a source for dissemination of bacteria.
Keywords: Nosocomial Infections, Nonfermentative Gram Negative, Stenotrphomonas Maltophilia, PCR
Hamid Reza Joshaghani , Saeid Parvizi , Khodaberdi Kalavi , Naser Behnampour, Hadi Joshaghani , Nader Hashemi, Sahar Alijanpour,
Volume 9, Issue 5 (Nov,Dec-2015 2015)
Abstract
Abstract
Background and Objective: Normal hemoglobin (Hb) is formed of a heme group and a protein group known as globin. Globin is made of four polypeptide chains and in hemoglobinopathies, the structure of one of these four polypeptide chain becomes abnormal. Cellulose acetate method is a common way to differentiate haemoglobinopathies. Inability to identify the components of Hb low concentrations and incapability to isolate all Hb types are among the disadvantages of this method. The aim of this study was to report the prevalence of hemoglobinopathies in the North of Iran by capillary electrophoresis method.
Methods: All patients with suspected hemoglobinopathies, referred by physicians for electrophoresis, have been studied in a private center in the city of Gorgan, Iran. The level of HbA2, HbA, HbF and other Hb was recorded.
Results: Overall, 725 blood samples were analyzed using the capillary method. HbE was reported in 2 patients, HbH was observed in 2 patients and Hb Barts was reported in 3 patients. Using the capillary method, among patients with the SDG area, only 4 of 38 (10.52%) had HbS and the majority of them (89.48%) had HbD.
Conclusion: HbD is the most common hemoglobinopathy in the North of Iran.
Keywords: Hemoglobinopathy; hemoglobin D; Capillary Electrophoresis; Iran
Nagmeh Alsabah Alavizadeh , Keyvan Hejazi, Tohid Mabhout Moghadam ,
Volume 12, Issue 4 (Jul-Aug 2018)
Abstract
ABSTRACT
Background and objectives: Visfatin is a newly characterized adipokine, which is highly expressed in visceral adipose tissue. The aim of this study was to evaluate effects of aerobic exercise training on visfatin, homocystein, C-reactive protein and lipid profile in sedentary men.
Methods: In this quasi-experimental study, 27 sedentary men were selected by convenience sampling method. The subjects were divided into a control group (n=13) and an aerobic training group (n=14). The subjects in the experimental group performed 60 minutes of aerobic exercise at 75-85% of maximum heart rate, three sessions per week for eight weeks. Data were analyzed in SPSS 16.0 (SPSS Inc., Chicago, IL, USA) using paired and independent sample t-test for comparison of means within and between groups, respectively. A P-value of less than 0.05 was considered statistically significant.
Results: The eight-week aerobic exercise training significantly reduced body weight (P=0.02), body mass index (P=0.01) and low-density lipoprotein-cholesterol (P=0.03) levels. It also caused a significant increase in the high-density-lipoprotein-cholesterol (P=0.00) and a significant decrease in visfatin levels (P=0.005). Serum homocysteine and C-reactive protein levels decreased by the end of the training program, but these changes were not statistically significant (P>0.05).
Conclusion: The eight-week aerobic exercise program could effectively improve lipid profile and visfatin levels. Therefore, this type of exercise could be recommended for prevention of cardiovascular disease and improvement of its risk factors.
Keywords: Aerobic, C-reactive protein, Exercise, Homocysteine.
Najmatol Sabah Alavizadeh, Amir Rashidlamir, Seyed Mahmood Hejazi ,
Volume 12, Issue 5 (Sep-Oct 2018)
Abstract
ABSTRACT
Background and objectives: Sirtuin-1 (SIRT1) is a conserved a NAD+-dependent protein deacetylase essential for cellular metabolism and defence against oxidative stress and endothelial dysfunction. This study aimed to compare effects of 8 weeks of aerobic and combined training on serum level of SIRT1 and PGC1-α in coronary artery bypass graft patients.
Methods: In this semi-experimental study, 45 non-active men (mean age 47.4 ± 3.3 years) who had undergone coronary artery bypass graft surgery were randomly divided into three groups: aerobic training, combined resistance and aerobic training and control. Trainings were performed at 80% of maximum heart rate, three sessions a week for 8 weeks. Blood samples were collected 24 hours before the first exercise session and 48 hours after the last training session. T-test and one-way analysis of variance were used for assessment of within-group and between-group differences, respectively. P-values less than 0.05 were considered as statistically significant.
Results: The 8-week aerobic and combined training increased serum levels of SIRT1 and PGC-1α compared with the control group, and significantly decreased the weight of subjects (p=0.001). There was no significant difference in serum levels of SIRT1 and PGC-1α between the two training groups (P≥0.05).
Conclusion: Both combined and aerobic training significantly improve the SIRT1 and PGC-1α levels in coronary artery bypass graft patients.
KEYWORDS: Combined training, Aerobic training, PGC-1α, SIRT1, CABG patient.
Najmeh Alsabah Alavizadeh , Amir Rashidlamir , Seyed Mahmud Hejazi ,
Volume 13, Issue 2 (Mar-Apr 2019)
Abstract
ABSTRACT
Background and Objectives: Cardiac rehabilitation improves functional capacity of post-coronary artery bypass grafting (CABG) patients. Therefore, the aim of this study was to evaluate effects of eight weeks of cardiac rehabilitation on serum levels of sirutin1 (SIRT1) and functional capacity of post-CABG patients.
Methods: Forty-five non-active men (mean age: 47.4 ± 3.3 years, duration of disease: 150 ± 69.12 months) were randomly divided into three equal groups: aerobic exercise, combined (resistance and aerobic) exercise and control. The subjects performed trainings at 60-75% of maximum heart rate, three sessions a week for eight weeks. Blood samples were collected 24 hours before the first exercise session and 48 hours after the last exercise session. Data were analyzed with SPSS 16.0 using one-way ANOVA and the Tukey post hoc test. P-values less than 0.05 were considered statistically significant.
Results: Aerobic and combined exercise increased serum SIRT1 levels and functional capacity. There was no significant decrease in body mass index (P=0.06). The SIRT1 level did not differ significantly between the two exercise groups after the intervention (P=0.91). There were significant difference between the study groups in terms of the travelled distance (P<0.034).
Conclusion: Both combined and aerobic exercise can increase serum levels of SIRT1, reduce body mass index and improve functional capacity in CABG patients.
Keywords: Resistance training, exercise, SIRT1.
Najmatol Sabah Alavizadeh, Amir Rashidlamir, Seyed Mahmoud Hejazi,
Volume 15, Issue 1 (Jan-Feb 2021)
Abstract
Background and objectives: Chemokines are a group of proteins involved in both innate and adaptive immunity with a significant role on homeostasis and immune system development. The present study aimed to evaluate effects of eight weeks of cardiopulmonary rehabilitation exercise after coronary artery bypass surgery on expression of chemokines CCL2 and CCL5 in peripheral blood mononuclear cells of middle-aged men.
Methods: The study was performed on 45 middle-aged men that had undergone coronary artery bypass surgery. The subjects were randomly divided into three groups of control (n=15), aerobic exercise (n=15) and combined aerobic-resistance exercise (n=15). The trainings were performed three times a week for eight weeks at 60-75% of target heart rate. Fasting blood samples were collected 24 hours before the first training session and 48 hours after the last training session. Lymphocytes were separated by centrifugation. Change in gene expression was investigated by real time-PCR. Data were analyzed in SPSS (version 16) using one-way analysis of variance and Tukey’s post hoc test.
Results: The eight week exercise training significantly decreased expression of CCL2 compared to the control group.
Conclusion: Rehabilitation exercise can be an effective way to prevent, control or reduce atherosclerosis by lowering expression of CCL2 and CCL5.
Afieh Samimi, Oghol Niaz Jorjani, Zohreh Sharifi, Faramarz Koohsar, Khodaberdi Kalavi, Fatemeh Mesgarian, Beniamin Talebi ,
Volume 16, Issue 3 (May-Jun 2022)
Abstract
Background and objectives: Cutaneous leishmaniasis is endemic in most areas of Iran, and the diagnosis of its species is essential for controlling the disease. Leishmania major is the causative agent for cutaneous leishmaniasis in humans. Molecular methods are generally more sensitive than microscopic methods. The present study aimed to use a polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) technique for detecting live L. major from wounds of patients with cutaneous leishmaniasis.
Methods: In the present study, a standard strain of L. major promastigotes was used as the positive control for purification of DNA. The Novy–MacNeal–Nicolle and RPMI-1640 media were used for reproduction of parasites. DNA was isolated from specimens taken from 35 patients with suspected cutaneous leishmaniasis whose disease was confirmed by direct smear method. The PCR-ELISA technique was later applied by using the standard strain, patient specimens, and primers specific for the 18s rRNA.
Results: Out of 35 patients, 17 (48.6%) were male and 18 (51.4%) were female. In addition, 8.6% of the patients lived in the Gonbad-e Kavus County, while all patients had been infected in villages around Gonbad-e Kavus. Of 35 patients with confirmed cutaneous leishmaniasis according to the direct smear method, 31 patients (86.31%) had leishmaniasis based on the PCR method and the PCR-ELISA methods.
Conclusion: Based on the results, the PCR-ELISA method is more sensitive and accurate for detecting L. major.
Khodaberdi Kalavi, Mohammad Ali Faghihi, Ogholniaz Jorjani , Zolikha Tatari,
Volume 16, Issue 5 (Sep-Oct 2022)
Abstract
Dear Editor,
There are several reports about the modulatory effects of intracellular pathogens, such as Leishmania spp., to help survival and replication inside host cells. They alter host cells' defense and offence mechanisms, and in case of leishmaniasis, the pathogen creates a hostile environment inside macrophages. We performed an RNA sequencing analysis of transcriptome changes in Leishmania major-infected human macrophages at four hour post infection. In this study, we investigated gene expression pattern of the infected macrophages against microbead (4.16 µm) polystyrene particles phagocytized and non-polarized macrophages as controls. Monocytes with high purity were isolated from healthy donors by magnetic-activated cell sorting and then differentiated into macrophages after 6-9 days of incubation at 37 ˚C.
In this study, we focused on some key interaction events between host cell and the pathogen; so some feature steps resulting from dictation strategies of the pathogen are presented below.
