Search published articles


Showing 4 results for Ahani Azari

Ania Ahani Azari , Taghi Zahraei Salehi , Bahar Nayeri Fasaei ,
Volume 10, Issue 6 (Nov-Dec-2016 2016)
Abstract

ABSTRACT

        Background and Objective: In this study, we compared the susceptibility of Salmonella typhimurium phoP mutant and its parent to stress conditions that the pathogen may encounter in a host.

        Methods: For this purpose, we used the phoP deletion mutant constructed in our previous study. In order to test the in vitro susceptibility of the mutant to stress factors, the effect of acidic pH, heat, bile salts and polymyxin on growth of the mutant was examined. Then, minimum inhibitory concentration and minimum bactericidal concentration of bile salts and polymyxin were determined. Salmonella typhimurium 14028 was used as the parent strain.

         Results: The mutant was highly susceptible to bile salts and polymyxin in comparison with the parent strain, but no difference was observed in their susceptibility to acid and heat.

          Conclusion: This study confirms the role of the phoP in resistance of Salmonella to polymyxin and bile salts. Mutation in the phoP leads to susceptibility of the mutant to bile and cationic antimicrobial peptides.

         Keywords: In vitro, Mutant, Salmonella typhimurium, Disease Susceptibility.


Abolfazl Khandan Del , Ania Ahani Azari , Ailar Jamalli, Ezzat Allah Ghaemi,
Volume 12, Issue 3 (May-Jun 2018)
Abstract

ABSTRACT
          Background and Objectives: Staphylococcus aureus is one of the most common causes of morbidity and mortality among intensive care unit (ICU) patients. Nasal carriage is one of the main routs of S. aureus transmission between hospital personnel and patients. The objective of this study was to evaluate the efficacy of mupirocin ointment in eradication of nasal carriage of S. aureus in the ICU staff and patients of Panje-Azar hospital in Gorgan, Iran.
          Methods: In the first three months of the study (January to March), the prevalence of S. aureus among ICU patients was determined by routine microbiological and biochemical testing. Nasal samples were taken from ICU staff and all patients recently admitted to the ICU. Mupirocin nasal ointment (2%) was applied for treatment of S. aureus nasal carriers. Post-treatment sampling was done after five weeks. During the next three months, the presence of S. aureus and rate of resistance to methicillin was evaluated in new patients admitted to the ICU using the method used previously.
          Results: Of 60 samples from the ICU staff, seven (11.7%) samples were positive for S. aureus. Moreover, of 240 samples from the ICU patients, two samples were found as S. aureus-positive. Of the nine S. aureus-positive isolates, only two (22.2%) were methicillin-resistant S. aureus (MRSA). In the pre-intervention sampling, only five samples (2.8%) were identified as S. aureus, two of which were MRSA. However, treatment with mupirocin ointment eradicated nasal carriage of S. aureus and no isolate was found after the intervention.
          Conclusion: Our finding showed that mupirocin nasal ointment is highly effective in eradication of S. aureus nasal carriage and subsequently contribute to reduction in frequency of nosocomial infections in the ICU.
          Keywords: Intensive Care Units, Mupirocin, Nasal, Staphylococcus aureus.

Hamidreza Pordeli, Hadi Shaki, Ania Ahani Azari, Muhammad Sadeqi Nezhad ,
Volume 12, Issue 4 (Jul-Aug 2018)
Abstract

 

ABSTRACT

            Background and objectives: Silver nanoparticles (AgNPs) are major nanomaterials with a variety of applications. The synthesis of nanoparticles by conventional methods is challenging and often requires use of hazardous chemicals. Therefore, there is a growing need for development of environmentally and economically friendly processes for the synthesis of nanoparticles. This study aimed at biosynthesis of AgNPs using a filamentous fungus; Fusarium solani.
            Methods: Twenty-four Fusarium isolates were found from several soil samples collected from depth of 1-10 cm. All isolates were identified as F. solani based on morphological characteristics. The synthesis of nanoparticles were evaluated after 24, 48, 72 hours of culture. AgNPs were characterized using UV-visible spectroscopy and transmission electron microscopy.
            Results: The synthesized AgNps showed maximum absorbance peak at 420 nm after 72 hours. Moreover, most AgNps were spherical with diameter of between 20 and 40 nm.
            Conclusion: In this study, we introduced a simple biological process for biosynthesis of AgNPs using F. solani isolates from soil samples. The results indicate that fungi may be suitable for safe and cost-effective production of AgNPs.
            Keywords: Fungi; Fusarium; Nanoparticles; Nanotechnology.

Ali Asghar Ayatollahi, Abolfazl Khandan Del, Ailar Jamalli, Khashayar Shahin, Ania Ahani Azari,
Volume 14, Issue 1 (Jan-Feb 2020)
Abstract

Background and objectives: Staphylococcus aureus is a frequent cause of hospital- and community-associated infections on a global scale. This organism is responsible for causing an extensive range of diseases and many of them are capable of biofilm formation for their survival. By this method, treatment of them with antibiotics become very difficult and antibiotic resistance is another rising concern.
Material and Methods: The clinical samples were collected and examined for Staphylococcus aureus by microbiological and biochemical tests. Then, the biofilm formation in Staphylococcus aureus isolates was detected by microtiter plate. Using SYBR Green Real-Time PCR, the expression of PSM was determined.
Results: A total of 60 strains of Staphylococcus aureus were isolated from clinical isolates. Of them, 47 strains (78.3%) were identified biofilm producing and the others were considered negative for biofilm formation. After real-time PCR testing to detect PSM, it was determined that 100% of the strains were positive for biofilms and PSM genes. The results of phenotypic and genotypic tests of biofilm were closely related to each other and the expression of PSM B gene was 80%. It was found that 100% of strains were biofilm producing and PSM B gene was present in 78.3% (47 strains) of them.
Conclusion: The prevalence of biofilm production in Staphylococcus aureus strains isolated from clinical samples was high, so it is highly important to monitor the prevalence of these organisms in hospitals and community as well as their antimicrobial resistance.

Page 1 from 1     

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.