This paper should
be cited as:Kiaei, MR, HedayatMofidi, M, Koohsar, F, Amini, A, Mirbazel, A, Hesari, Z
Comparison of Two Methods: Qualitative and Quantitative
Study of C - Reactive Protein
Kiaei, MR. (BSc)1,
HedayatMofidi, M. (MSc)2, Amini,
A. (MSc)3, Koohsar, F. (MSc)*4, Hoseinzadeh,
S. (BSc)5, Mirbazel, A.6, Hesari, Z. (MSc)7
1. BSc of
Medical Laboratory, Paramedical School, Golestan
University of Medical Sciences, Gorgan, Iran
2. MSc of
Immunology, Paramedical School, Golestan University
of Medical Sciences, Gorgan, Iran
3. PhD Student
of Parasitology, Paramedical School, Golestan
University of Medical Sciences, Gorgan, Iran
4. MSc of
Microbiology, Paramedical School, Golestan University
of Medical Sciences, Gorgan, Iran
5. MSc Student
of Biochemistry, Paramedical School, Golestan
University of Medical Sciences, Gorgan, Iran
6. Laboratory
Technician of Talghani Hospital, Golestan
University of Medical Sciences, Gorgan, Iran
7. PhD Student
of Biochemistry, School of Medicine, Iran University of Medical Sciences,
Tehran, Iran
Abstract
Background
and Objective:C-reactive protein (CRP) is an
acute phase protein produced in liver. It is less than 5 mg per deciliter in
the serum and body fluids of normal individuals, but it is increased suddenly
within a few hours following inflammatory reaction. In bacterial and viral
infections, active rheumatic fever, acute myocardial infarction and rheumatoid
arthritis are also increased. The aim of this study was to investigate CRP
level by Qualitative and Quantitative methods.
Material and
Methods: The CRP of 200 patients was investigated by quantitative and
qualitative methods. Qualitative CRP testing was conducted three times by
different people, using two kit of bionic and Omega, and then the mean of the
results was reported.For quantitative CRP testing, Immunoturbidimetry was used.
Results: In qualitative CRP test by Bionic kit:180 (90%) were negative, 6 (3%) weakly positive, 9 (4.5%)
+1and 5 (2.5 %) were + 2. In qualitative CRP test by Omega Kit: 148 (74%) were
negative, 32 (16%) weakly positive, 13 (6.5%) +1, 4 (2%) +2 and 3 (1.5%) were
+3. A high percentage of Qualitative results, which were weakly positive,
became negative by Quantitative methods.
The Qualitative results of +1 and the next became positive by
Quantitative methods.
Conclusion: It seems that in the early stages of inflammatory
disease, quantitative methods are preferred to qualitative methods. Also, in
cases that the CRP test results are weakly positive by qualitative methods,
they should be controlled by quantitative methods too.
Keywords: CRP; CRP Test Quantitative; Qualitative CRP Test
Corresponding
Author: Koohsar, F
Email:
fkoohsar@yahoo.com
Received:
6 May 2013 Revised: 19 Dec 2013 Accepted: 22 Dec 2013