Thu, Apr 18, 2024
Volume 11, Issue 2 (Mar-Apr 2017)
mljgoums 2017, 11(2): 30-35 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Komijani M, Bouzari M, Rahimi F. Detection of TEM, SHV and CTX-M Antibiotic Resistance Genes in Escherichia coli Isolates from Infected Wounds. mljgoums 2017; 11 (2) :30-35
URL: http://mlj.goums.ac.ir/article-1-972-en.html
URL: http://mlj.goums.ac.ir/article-1-972-en.html
1- Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
2- Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran , bouzari@sci.ui.ac.ir
2- Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran , bouzari@sci.ui.ac.ir
Abstract: (13829 Views)
ABSTRACT
Background and Objective: Escherichia coli is one of the most common causes of hospital-acquired infections. Extended-spectrum β-lactamase (ESBL)-producing E. coli strains are resistant to third-generation cephalosporins. The three main genes involved in ESBL production are TEM, SHV and CTX-M. Detection of ESBL-producing E. coli is of importance for infection control, reduction of excessive antibiotic use and epidemiological surveillance. This study aimed to detect ESBL-producing E. coli strains isolated from wound infections using phenotypic and molecular methods.
Methods: During 2013- early 2015, 86 strains were collected from three hospitals in Isfahan, Iran. Antibiotic susceptibility testing was done using ceftazidime and ceftazidime + clavulanic acid discs. Polymerase chain reaction was used for the detection of the three resistance genes.
Results: The resistance genes SHV, CTX-M and TEM were detected in 49 isolates (56.9%). In addition, 39 isolates (45%) were ESBL-producing strains. According to the results, 5 (5.8%), 14 (16.2%), 19 (22%) and 11 (12.7%) isolates contained the SHV, CTX-M, TEM and CTX-M + TEM genes, respectively. The frequency of CTX and TEM were significantly higher than that of SHV gene (P <0.05). Most of the isolated bacteria were resistant to cefazolin and sensitive to nitrofurantoin.
Conclusions: There is a difference between the frequency of ESBL-positive isolates reported in the phenotypic and genotypic methods, which could be due to the lower sensitivity of the phenotypic method and impact of environmental factors on the emergence of antibiotic resistance.
Keywords: Antibiotic resistance genes, ESBL, TEM, SHV, CTX-M, Escherichia coli.
Background and Objective: Escherichia coli is one of the most common causes of hospital-acquired infections. Extended-spectrum β-lactamase (ESBL)-producing E. coli strains are resistant to third-generation cephalosporins. The three main genes involved in ESBL production are TEM, SHV and CTX-M. Detection of ESBL-producing E. coli is of importance for infection control, reduction of excessive antibiotic use and epidemiological surveillance. This study aimed to detect ESBL-producing E. coli strains isolated from wound infections using phenotypic and molecular methods.
Methods: During 2013- early 2015, 86 strains were collected from three hospitals in Isfahan, Iran. Antibiotic susceptibility testing was done using ceftazidime and ceftazidime + clavulanic acid discs. Polymerase chain reaction was used for the detection of the three resistance genes.
Results: The resistance genes SHV, CTX-M and TEM were detected in 49 isolates (56.9%). In addition, 39 isolates (45%) were ESBL-producing strains. According to the results, 5 (5.8%), 14 (16.2%), 19 (22%) and 11 (12.7%) isolates contained the SHV, CTX-M, TEM and CTX-M + TEM genes, respectively. The frequency of CTX and TEM were significantly higher than that of SHV gene (P <0.05). Most of the isolated bacteria were resistant to cefazolin and sensitive to nitrofurantoin.
Conclusions: There is a difference between the frequency of ESBL-positive isolates reported in the phenotypic and genotypic methods, which could be due to the lower sensitivity of the phenotypic method and impact of environmental factors on the emergence of antibiotic resistance.
Keywords: Antibiotic resistance genes, ESBL, TEM, SHV, CTX-M, Escherichia coli.
Research Article: Original Paper |
Received: 2017/08/2 | Accepted: 2017/08/2 | Published: 2017/08/2 | ePublished: 2017/08/2
Received: 2017/08/2 | Accepted: 2017/08/2 | Published: 2017/08/2 | ePublished: 2017/08/2
References
1. Bowler P, Duerden B, Armstrong D. Wound microbiology and associated approaches to wound management. Clinical Microbiology Reviews. 2001; 14(2): 244-69. doi: 10.1128/CMR.14.2.244-269.2001. [DOI:10.1128/CMR.14.2.244-269.2001]
2. Johora FT, Ahmed Z. Bacteriological study of post-operative abdominal wound infection-a Case Study. Bangladesh Journal of Medical Science. 2013; 12(1): 86-90. DOI: http://dx.doi.org/10.3329/bjms.v12i1.10982. [DOI:10.3329/bjms.v12i1.10982]
3. Jacobsen S, Stickler D, Mobley H, Shirtliff M. Complicated catheter-associated urinary tract infections due to Escherichia coli and Proteus mirabilis. Clinical Microbiology Reviews. 2008; 21(1): 26-59. doi: 10.1128/CMR.00019-07. [DOI:10.1128/CMR.00019-07]
4. Roca I, Akova M, Baquero F, Carlet J, Cavaleri M, Coenen S, et al. The global threat of antimicrobial resistance: science for intervention. New Microbes New Infect. 2015; 6: 22-9. doi: 10.1016/j.nmni.2015.02.007. [DOI:10.1016/j.nmni.2015.02.007]
5. Lu TK, Koeris MS. The next generation of bacteriophage therapy. Current Opinion in Microbiology. 2011; 14(5): 524-31. doi: 10.1016/j.mib.2011.07.028. [DOI:10.1016/j.mib.2011.07.028]
6. Alfaresi MS, Elkoush AA, Alshehhi HM, Abdulsalam AI. Molecular characterization and epidemiology of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates in the United Arab Emirates. Med Princ Pract. 2011; 20(2): 177-80. doi: 10.1159/000319912. [DOI:10.1159/000319912]
7. Kaftandzieva A, Trajkovska-Dokic E, Kotevska V, Cekovska Z, Jankoska G. Genotypes of ESBL producing Escherichia coli and Klebsiella pneumoniae in relation to resistance to antimicrobial drugs. Pril (Makedon Akad Nauk Umet Odd Med Nauki). 2014; 35(2): 31-8. [DOI:10.2478/prilozi-2014-0004]
8. Shaikh S, Fatima J, Shakil S, Rizvi SMD, Kamal MA. Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi Journal of Biological Sciences. 2015; 22(1): 90-101. [DOI:10.1016/j.sjbs.2014.08.002]
9. Rottier WC, Ammerlaan HS, Bonten MJ. Effects of confounders and intermediates on the association of bacteraemia caused by extended-spectrum β-lactamase-producing Enterobacteriaceae and patient outcome: a meta-analysis. J Antimicrob Chemother. 2012; 67(6): 1311-20. doi: 10.1093/jac/dks065. [DOI:10.1093/jac/dks065]
10. Garrec H, Drieux-Rouzet L, Golmard J-L, Jarlier V, Robert J. Comparison of nine phenotypic methods for detection of extended-spectrum β-lactamase production by Enterobacteriaceae. J Clin Microbiol. 2011; 49(3): 1048-57. doi: 10.1128/JCM.02130-10. [DOI:10.1128/JCM.02130-10]
11. Krishnamurthy V, Vijaykumar G, Kumar S, Prashanth H, Prakash R, Nagaraj E. Phenotypic and genotypic methods for detection of extended spectrum β lactamase producing Escherichia coli and Klebsiella pneumoniae isolated from ventilator associated pneumonia. Journal of Clinical and Diagnostic Research: JCDR. 2013; 7(9): 1975. doi: 10.7860/JCDR/2013/6544.3376. [DOI:10.7860/JCDR/2013/6544.3376]
12. Kazemnia A, Ahmadi M, Dilmaghani M. Antibiotic resistance pattern of different Escherichia coli phylogenetic groups isolated from human urinary tract infection and avian colibacillosis. Iranian Biomedical Journal. 2014; 18(4): 219-24.
13. Cockerill FR, Clinical, Institute LS. Performance standards for antimicrobial susceptibility testing: twenty-second informational supplement. National committee for clinical laboratory standards; 2012.
14. Kim J, Jeon S, Rhie H, Lee B, Park M, Lee H, et al. Rapid detection of extended spectrum β-lactamase (ESBL) for Enterobacteriaceae by use of a multiplex PCR-based method. Infection and Chemotherapy. 2009; 41(3):181-4. DOI: 10.3947/ic.2009.41.3.181. [DOI:10.3947/ic.2009.41.3.181]
15. Sidjabat HE, Paterson DL, Adams-Haduch JM, Ewan L, Pasculle AW, Muto CA, et al. Molecular epidemiology of CTX-M-producing Escherichia coli isolates at a tertiary medical center in western Pennsylvania. Antimicrob Agents Chemother. 2009; 53(11): 4733-9. doi: 10.1128/AAC.00533-09. [DOI:10.1128/AAC.00533-09]
16. Bhattacharjee A, Sen MR, Prakash P, Anupurba S. Role of β-lactamase inhibitors in enterobacterial isolates producing extended-spectrum β-lactamases. J Antimicrob Chemother. 2008; 61(2): 309-14. doi: 10.1093/jac/dkm494. [DOI:10.1093/jac/dkm494]
17. Rekha R, Alam Rizvi M, Jaishree P. Designing and validation of genus-specific primers for human gut flora study. Electronic Journal of Biotechnology. 2006;9(5):0-. [DOI:10.2225/vol9-issue5-fulltext-2]
18. Bokaeian M, Zahedani SS, Bajgiran MS, Moghaddam AA. Frequency of PER, VEB, SHV, TEM and CTX-M genes in resistant strains of Pseudomonas aeruginosa producing extended spectrum β-lactamases. Jundishapur Journal of Microbiology. 2015; 8(1). e13783. doi: 10.5812/jjm.13783. [DOI:10.5812/jjm.13783]
19. Mendonça N, Leitão J, Manageiro V, Ferreira E, Caniça M. Spread of extended-spectrum β-lactamase CTX-M-producing Escherichia coli clinical isolates in community and nosocomial environments in Portugal. Antimicrobial Agents and Chemotherapy. 2007; 51(6): 1946-55. doi: 10.1128/AAC.01412-06. [DOI:10.1128/AAC.01412-06]
20. Fatemi S-M, Doosti A, Tavakoli H, Moayednia R, Ghasemi-Dehkordi P, Kelidari B, et al. Antibiotic susceptibility patterns of isolated bacteria from bile fluids of patients with gallstone disease in Isfahan city (Iran). Archives of Biological Sciences. 2015; 67(2): 611-7. DOI: 10.2298/ABS140506021F. [DOI:10.2298/ABS140506021F]
21. Etok CA, Edem EN, Ochang E. Aetiology and antimicrobial studies of surgical wound infections in university of Uyo teaching hospital (UUTH), Uyo, Akwa Ibom state, Nigeria. Open Access Scientific Reports. 2012; 1: 341. doi:10.4172/scientificreports.341.
22. Islam MS, Yusuf MA, Islam MB, Jahan WA. Frequency of ESBL in Surgical Site Infection at a Tertiary Care Hospital. Journal of current and advance medical research. 2014; 1(2): 25-9. [DOI:10.3329/jcamr.v1i2.20514]
23. Rezai MS, Salehifar E, Rafiei A, Langaee T, Rafati M, Shafahi K, et al. Characterization of multidrug resistant extended-spectrum beta-lactamase-producing Escherichia coli among uropathogens of pediatrics in north of Iran. Biomed Res Int. 2015; 2015: 309478. doi: 10.1155/2015/309478. [DOI:10.1155/2015/309478]
24. Memariani M, Peerayeh SN, Salehi TZ, Mostafavi SKS. Occurrence of SHV, TEM and CTX-M β-Lactamase genes among enteropathogenic Escherichia coli strains isolated from children with diarrhea. Jundishapur J Microbiol. 2015; 8(4): e15620. doi: 10.5812/jjm.8(4)2015.15620. [DOI:10.5812/jjm.8(4)2015.15620]
25. Varkey DR, Balaji V, Abraham J. Molecular characterisation of extended spectrum beta lactamase producing strains from blood sample. International Journal of Pharmacy and Pharmaceutical Sciences. 2014; 6(3): 276-8. doi: 10.1186/s12941-015-0098-9. [DOI:10.1186/s12941-015-0098-9]
26. Rodríguez-Ba-o J, Alcalá J, Cisneros JM, Grill F, Oliver A, Horcajada JP, et al. Escherichia coli producing SHV-type extended-spectrum β-lactamase is a significant cause of community-acquired infection. J Antimicrob Chemother. 2009; 63(4): 781-4. doi: 10.1093/jac/dkp028. [DOI:10.1093/jac/dkp028]
27. Fang H, Ataker F, Hedin G, Dornbusch K. Molecular epidemiology of extended-spectrum β-lactamases among Escherichia coli isolates collected in a Swedish hospital and its associated health care facilities from 2001 to 2006. Journal of Clinical Microbiology. 2008; 46(2): 707-12. doi: 10.1128/JCM.01943-07. [DOI:10.1128/JCM.01943-07]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Enamad
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.