Volume 10, Issue 4 (Jul-Aug 2016 2016)                   mljgoums 2016, 10(4): 40-45 | Back to browse issues page

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Khodadadi P, Bizhanzadeh M, Najafi A, Zarinpour V, Moshfe A, Ansari H. Antibiotic Resistance and Detection of femA Gene in Staphylococcus Aureus Isolates from Raw Milk. mljgoums 2016; 10 (4) :40-45
URL: http://mlj.goums.ac.ir/article-1-877-en.html
Antibiotic Resistance and Detection of femA Gene in Staphylococcus Aureus Isolates from Raw Milk
Pouya Khodadadi 1, Mehdi Bizhanzadeh2 , Akram Najafi3 , Vajiheh Zarinpour4 , Abdolali Moshfe5 , Hossein Ansari6
1- Department of Microbiology, Islamic Azad University, Masjed-Soliman Branch, Masjed-Soliman, Iran , hosseinansari62@gmail.com
2- Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3- Department of Marine Microbiology, Persian Gulf Marine Biotechnology Medicine Research Center, Bushehr University of Medical Sciences, Bushehr, Iran
4- Department of Genetic, Faculty of Science, Islamic Azad University, Damghan Branch, Damghan, Iran
5- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
6- Department of Microbiology, Islamic Azad University, Masjed-Soliman Branch, Masjed-Soliman, Iran
Abstract:   (13376 Views)

ABSTRACT

        Background and Objective: Antibiotic-resistant Staphylococcus aureus strains have become a problem in treatment of infections caused by S. aureus. This study aimed to evaluate antibiotic resistance in S. aureus isolates from raw milk and detect femA gene in these isolates, as a confirmatory test for identification of S. aureus species.

        Methods: This cross-sectional study was performed on 110 raw milk samples. After culture in Cooked Meat broth, presence of S. aureus in grown colonies was confirmed in accordance with Iranian National Standard, No. 1194. Antibiotic resistance was then evaluated according to guidelines recommenced by the Clinical Laboratory Standards Institute. FemA-specific polymerase chain reaction was performed on antibiotic-resistant strains using specific primers and standard strains to differentiate S. aureus from other species.

         Results: S. aureus were found in 43 (39.09%) of the 110 collected samples. Among these isolates, 79.07% and 76.75% were phenotypically resistant to penicillin and ceftazidime, respectively. In addition, the femA gene was detected in all isolates.

          Conclusion: The results of this study show a high prevalence of resistance to penicillin and ceftazidime among S. aureus strains isolated from raw milk.

        Keywords: Staphylococcus aureus, Antibiotic Resistance, PCR.

Keywords: Staphylococcus aureus, Antibiotic Resistance, PCR.
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Research Article: Original Paper |
Received: 2016/10/26 | Accepted: 2016/10/26 | Published: 2016/10/26 | ePublished: 2016/10/26
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.