Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)                   mljgoums 2015, 9(2): 32-38 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Zare, M, Karimi Akhormeh, A, Takhshid, MA. Qualitative and Quantitative Approaches for Determination of Hemolyzed Serum. mljgoums 2015; 9 (2) :32-38
URL: http://mlj.goums.ac.ir/article-1-679-en.html
1- Diagnostic Laboratory Sciences and Technology Research Center, Student Research Committee, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
2- School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran , Takhshid2001@yahoo.co.uk
Abstract:   (10606 Views)

Abstract

Background and Objective: Prevention of medical laboratory errors is a major goal of quality control programs. Hemolyzed specimen is one of the common issues causing medical laboratory errors. Apart from the visual assessment, the measurement of serum hemoglobin concentration can be another method to evaluate the intensity of hemolysis. We aimed to assess hemolyzed serum specimens using two quantitative and qualitative methods.

Material and Methods: the serum samples (n=890) were evaluated for the presence and degree of hemolysis, using quantitative and qualitative methods. In qualitative method, the samples were investigated visually and in quantitative with the measurement of serum free hemoglobin concentration. Furthermore, the relative frequency of hemolyzed specimens was calculated.

 Results: the hemolyzed samples were 23.4 % in qualitative and 65.8% in quantitative method.  In quantitative, 71.2% of the specimens had mild hemolysis (sfHB<50 mg/dL), 26.8% intermediate (50mg/dL< sfHB <250mg/dL), and 2% high (sfHB >250 mg/dL). The percentage of hemolyzed specimens was higher in intensive care unit than those of other departments (P < 0.05).

Conclusion: given that hemolysis in small amount is not detectable visually, we recommend using quantitative method for evaluating hemolyzed specimens.

Keywords: Hemolysis, Diagnostic Errors, Qualitative Research, Quantitative Research

Research Article: Original Paper |
Received: 2015/07/21 | Accepted: 2015/07/21 | Published: 2015/07/21 | ePublished: 2015/07/21

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.