Volume 10, Issue 6 (Nov-Dec-2016 2016)                   mljgoums 2016, 10(6): 43-48 | Back to browse issues page

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Komeilifard L, Hemayat khahjahromi V. Effects of Citrus aurantium Fruit Extract on Spermatogenesis in Streptozotocin-Induced Diabetic Rats. mljgoums 2016; 10 (6) :43-48
URL: http://mlj.goums.ac.ir/article-1-912-en.html
1- Department of Biology, Jahrom Beranch
Abstract:   (8040 Views)


          Background and Objectives: Diabetes is one of the most common endocrine disorders, which is associated with changes in testicular tissue. The present study investigated therapeutic and prophylactic properties of bitter orange (Citrus aurantium) juice on testicular tissue and spermatogenesis process.

          Methods: Forty streptozotocin-induced diabetic Wistar rats aged three months with mean weight of 170-200 g were divided into 4 groups including 1) control group, 2) diabetic control group, 3) diabetic group receiving 100mg/kg C. aurantium extract and 4) diabetic group receiving 200 mg/kg C. aurantium extract. The extract was administered to the rats for 56 days by gavage. After this period, the rats were anesthetized with ether and then their testes were fixed in 10% formalin for sample preparation. The testicular tissue was examined by haematoxylin and eosin staining under a light microscope with 10 and 40 magnifications. The mean number of Leydig and Sertoli cells, spermatogonia, spermatocytes and spermatids were calculated.

           Results: A significant decrease was observed in mean weight of left testis in diabetic rats compared to that of controls (P≤0.05). The mean weight of testes showed no significant difference in diabetic rats treated with 200 mg/kg of extract compared with the control group. Diabetes reduced the number of spermatogonia, spermatocytes, spermatids and Sertoli cells. The number of cells increased significantly in the diabetic group receiving 200 mg/kg of extract. The spermatocytes and spermatids in both groups treated with the extract increased significantly.

          Conclusion: This study shows the positive effect of bitter orange extract on complications of diabetes in testicular tissue. Therefore, this extract could be used for therapeutic purposes.

           Keywords: diabetes, bitter orange juice, spermatogenesis, testis, rat

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Research Article: Original Paper |
Received: 2016/01/25 | Accepted: 2016/01/25 | Published: 2016/09/25 | ePublished: 2016/09/25

1. Harrison T R, Jameson L J, Kasper L D, Longo D, Fouci AS, Wilson J. Harrison's Principles of Medicine. 15th ed. Metabolic and Endocrine Disorders, McGraw- Hill's Access Mediine. 2001; 11-18.
2. Andreoli TE, Claud BJ.Cecil Essentials of Medicine. WB Sanders Company, Philadelphia, 5th ed. 2000; 19-39.
3. Bayness JW.Role of oxidative strees in development of application in diabetes. 1991; 40(4): 405-411.
4. Gutteride JMC.Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem. 1995; 41(12): 1819-1828.
5. Soudamani S, Yuvaraj S, Rengarajan S, Sivakumar R, Malini T, Balasubramanian K. Effects of streptozotocin diabetes and insulin replacement on androgen and estrogen receptor concentrations in the epididymis of Wistar rats. JER. 2006; 10(1): 59-61.
6. Balasubramanian K, Sivashanmugam P, Thameemdheen S, Govindarajulu P. Effect of diabetes mellitus on epididymal enzymes of adult rats. Indian J Exp Biol. 1991; 29(10): 907-9.
7. Ballester J, Munoz MC, Dominguez J, Rigau T, Guinovart JJ, Rodriguez-Gil JE. Insulin-dependent diabetes affects testicular function by FSH- and LH linked mechanisms. J Androl. 2004; 25(5): 706-19. [DOI:10.1002/j.1939-4640.2004.tb02845.x]
8. Baccetti B, La Marca A, Piomboni P, Capitani S, Bruni E, Petraglia F et al. Insulin-dependent diabetes in men is associated with hypothalamo-pituitary dearangement and with impairment in semen quality. Hum Reprod. 2002; 17(10): 2673-7. [DOI:10.1093/humrep/17.10.2673]
9. Murray FT, Cameron DF, Orth JM, Katovich MJ. Gonadal dysfunction in the spontaneously diabetic BB rats: alteration of the testes morphology, serum testosterone and LH.HormMetab Res. 1985; 17(10): 495-501. [DOI:10.1055/s-2007-1013588]
10. Huang YT, Wang GF, Chen CF, Chen CC, Hong CY, Yang MC. Fructus aurantii reduced portal pressure in portal hypertensive rats. Life Sci. 1995; 57(22): 2011-20. [DOI:10.1016/0024-3205(95)02195-O]
11. Mahmoodi M, ShamsiMeimandi M, Foroumadi AR, Raftari Sh, AsadiShekari M. Antidepressant effect of sour orange flowers extract on lipopolysaccharide-i. Journal of Kerman University of Medical Sciences. 2005; 12(4): 244-51. [Persian]
12. Monsef–esfahani HR, Amanzade Y, Alhani Z, Hajimehdipour H, Faramarzi MA. GC/MS Analysis of Citrusaurantium L. Hydrolate and its companant with the commercial samples. Iranian Journal of pharmaceutical Research.2004; 3(3): 177-179.
13. Ballester J, Munoz MC, Dominguez J, Palomo MJ, Rivera M , Rigau T. Tungstate administration improves the sexual and reproductive function in female rats with streptozotocin induced diabetes. Hum Reprod. 2007; 22(8): 2128-2135. [DOI:10.1093/humrep/dem168]
14. Mcanally B W. Demonstration of in-vitro antiviral of action of Acemannan against multiple viruses including the HIV virus. Presented at International conference on AIDS. 1988.
15. Davis RH, Rosenthal KY.Processed aloe vera administered topically inhibits inflamentation. JAm Pediatric Medical ASSn. 1989; 79(8): 395-397.
16. Sharma N, Jacob D.Antifertility investigation andtoxilcological screening of the petroleum ether extract ofthe leaves of Mentha arvensis L. in male albino mice. JEthnopharmacol. 2001;75(1):5-12. [DOI:10.1016/S0378-8741(00)00362-7]
17. Giugliano D, Ceriello A, Paolisso G. Oxidative stress and diabetic vascular complications. Diabetes Care. 1996; 19(3): 257-67. [DOI:10.2337/diacare.19.3.257]
18. Chen CS, Chao HT, Pan RL, Wei YH. Hydroxyl radicalinduced decline in motility and increase in lipid peroxidation and DNA modification in human sperm. BiochemMolBiol Int. 1997; 43(2): 291-303.
19. Guneli E, Tugyan K, Ozturk H, Gumustekin M, Cilaker S, Uysal N. Effect of melatonin on testicular damage in streptozotocin-induced diabetes rats.EurSurg Res. 2008; 40: 354-60.doi: 10.1159/000118032. [DOI:10.1159/000118032]
20. Cai L, Hales BF, Robaire B. Induction of apoptosis in the germ cells of adult male rats after exposure to cyclophosphamide.BiolReprod. 1997; 56(6): 1490-7. [DOI:10.1095/biolreprod56.6.1490]
21. Turk G, Sonmez M, Aydin M, Yuce A, Gur S, Yuksel M, et al. Effects of Pomegranate Juice Consumption on Sperm Quality Spermatogenic Cell Density Antioxidant Activity and Testosterone Level in Male Rats.ClinNutr. 2008;27(2):289-961.doi: 10.1016/j.clnu.2007.12.006. [DOI:10.1016/j.clnu.2007.12.006]
22. Tribble DL. Antioxidant consumption and risk of coronary heart disease: emphasis on vitamin C, vitamin E, and-βCarotene: a statement for healthcare professionals form American Heart Association. Circulation. 1999; 99(4): 591-95. [DOI:10.1161/01.CIR.99.4.591]
23. Aitken RJ, Clarkson JS.Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J ReprodFertil. 1987; 81(2): 459-69. [DOI:10.1530/jrf.0.0810459]
24. Satoh Y, Tashiro S, Satoh M, Fujimoto Y, Xu JY,Ikekawa T. Studies on the bioactive constituents of Fructus Aurantii Immaturus.YakugakuZasshi. 1996;116(3):244-50. [DOI:10.1248/yakushi1947.116.3_244]
25. Bocco A, Cuvelier ME, Richard H, Berset C. Antioxidant activity and phenolic composition of citrus peel and seed extract. J Agric Food CHem. 1998; 46(6):2123-9. [DOI:10.1021/jf9709562]
26. Goli Z, Lakzaei M, Pouramir M. Antioxidant activity of sour orange peel extract and its effect on lipid oxidation in raw and cooked fish Hypophthalmichthysmolitrix fish.Iran J NutrSci Food Technol. 2010;5(2):19-26.

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