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Volume 15, Issue 5 (Sep-Oct 2021)
mljgoums 2021, 15(5): 27-31 |
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Zehtabian S, alibakhshi R, Seyedena S Y, Rai A. Development of a New Framework for Health Assessment in Patients with Coronary Artery Disease by using miRNA-197 in Adults. mljgoums 2021; 15 (5) :27-31
URL: http://mlj.goums.ac.ir/article-1-1304-en.html
URL: http://mlj.goums.ac.ir/article-1-1304-en.html
1- Department of Biology, Faculty of Biological Science, Islamic Azad University, North Tehran Branch, Tehran, Iran
2- kermanshah university of medical sciences , ralibakhshiy@gmail.com
3- Department of biology, faculty of Biological science, Islamic Azad University, North Tehran Branch, Tehran, Iran
4- kermanshah university of medical sciences
2- kermanshah university of medical sciences , ralibakhshiy@gmail.com
3- Department of biology, faculty of Biological science, Islamic Azad University, North Tehran Branch, Tehran, Iran
4- kermanshah university of medical sciences
Abstract: (3182 Views)
Background and objectives: Coronary artery disease (CAD) refers to stenosis or obstruction of coronary artery due to atherosclerosis or clotting. The aim of this study was to evaluate possible association of serum miRNA-197 (miR-197) expression as a biomarker for CAD diagnosis.
Methods: In this study, 100 patients with CAD who had angiography and vascular transplantation were selected. Expression of miR-197 was evaluated using real-time RT-PCR technique and the SYBR Green method. The Pearson's correlation coefficient was used to determine relationship of miR-197 expression and severity of coronary artery disease. The t-test was used to determine significance of expression of miR-197 in the study groups. All statistical analyses were carried out in SPSS 16 and at significance of 0.05.
Results: The results showed a direct relationship between miR-197 expression and CAD severity. The relative expression of miR-197 in the CAD patients was significantly higher than that in control subjects (P<0.004).
Conclusion: It seems that miR-197 can be considered as an indicator of coronary endothelial cell function. This microRNA could be used as a biomarker for CAD prognosis and treatment progression.
Methods: In this study, 100 patients with CAD who had angiography and vascular transplantation were selected. Expression of miR-197 was evaluated using real-time RT-PCR technique and the SYBR Green method. The Pearson's correlation coefficient was used to determine relationship of miR-197 expression and severity of coronary artery disease. The t-test was used to determine significance of expression of miR-197 in the study groups. All statistical analyses were carried out in SPSS 16 and at significance of 0.05.
Results: The results showed a direct relationship between miR-197 expression and CAD severity. The relative expression of miR-197 in the CAD patients was significantly higher than that in control subjects (P<0.004).
Conclusion: It seems that miR-197 can be considered as an indicator of coronary endothelial cell function. This microRNA could be used as a biomarker for CAD prognosis and treatment progression.
Keywords: MIRN197 microRNA [MeSH], human [MeSH], Coronary Artery Disease [MeSH], U6 small nuclear RNA [MeSH]
Research Article: Original Paper |
Subject:
Molecular Medicine
Received: 2020/06/24 | Accepted: 2020/09/19 | Published: 2021/08/31 | ePublished: 2021/08/31
Received: 2020/06/24 | Accepted: 2020/09/19 | Published: 2021/08/31 | ePublished: 2021/08/31
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