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mljgoums 2020, 14(6): 48-54 |
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Chizari M, Hosseini S M, Mohammadi A, Fakhari J, Mashhadi Mohammadzadeh-Vazifeh M. Evaluation of Microbial Contamination of Dried Black Curd (Gharahghorut) Produced in Iran. mljgoums 2020; 14 (6) :48-54
URL: http://mlj.goums.ac.ir/article-1-1293-en.html
URL: http://mlj.goums.ac.ir/article-1-1293-en.html
Mojtaba Chizari1 
, Seyed Masoud Hosseini 2, Ali Mohammadi3 , Javad Fakhari1 , Mojtaba Mashhadi Mohammadzadeh-Vazifeh1
, Seyed Masoud Hosseini 2, Ali Mohammadi3 , Javad Fakhari1 , Mojtaba Mashhadi Mohammadzadeh-Vazifeh1
1- Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
2- Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran , ma_hosseini@sbu.ac.ir
3- Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
2- Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran , ma_hosseini@sbu.ac.ir
3- Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Abstract: (5320 Views)
Background and objectives: Dried Black Curd (DBC), also known as Gharahghorut (Persian), is a dairy product produced from curd of yogurt or doogh. The aim of this study was to evaluate microbial contamination of industrially and traditionally produced DBC in Iran.
Methods: Four DBC brands holding certification of the Institute of Standards and Industrial Research of Iran (ISIRI), Iran Ministry of Health and Medical Education (IMHME) and ISO were purchased from a market in Tehran, Iran. Microbial monitoring for presence of coliform, Escherichia coli, coagulase-positive Staphylococcus aureus, mold and yeast was performed using different basic, enriched, selective and differential media including peptone water agar, MacCankey agar, blood agar and brain heart infusion agar, etc. according to the ISIRI standards (No.13299).
Results: None of the tested samples had microbial contamination. However, in one traditional DBC sample, the population of mold and yeast was higher than the acceptable level defined by the ISIRI standard.
Conclusion: We found no contamination with coagulase-positive S. aureus, E. coli and coliforms in four brands of traditionally and industrially produced DBC in Iran. This indicates that the hygienic practices designated by the IMHME are well-implemented in the industrial sector. However, the mold and yeast contamination in traditionally produced DBC should be prevented by applying hygienic practices during the process of manufacturing and distribution.
Methods: Four DBC brands holding certification of the Institute of Standards and Industrial Research of Iran (ISIRI), Iran Ministry of Health and Medical Education (IMHME) and ISO were purchased from a market in Tehran, Iran. Microbial monitoring for presence of coliform, Escherichia coli, coagulase-positive Staphylococcus aureus, mold and yeast was performed using different basic, enriched, selective and differential media including peptone water agar, MacCankey agar, blood agar and brain heart infusion agar, etc. according to the ISIRI standards (No.13299).
Results: None of the tested samples had microbial contamination. However, in one traditional DBC sample, the population of mold and yeast was higher than the acceptable level defined by the ISIRI standard.
Conclusion: We found no contamination with coagulase-positive S. aureus, E. coli and coliforms in four brands of traditionally and industrially produced DBC in Iran. This indicates that the hygienic practices designated by the IMHME are well-implemented in the industrial sector. However, the mold and yeast contamination in traditionally produced DBC should be prevented by applying hygienic practices during the process of manufacturing and distribution.
Research Article: Original Paper |
Subject:
Microbiology
Received: 2020/04/24 | Accepted: 2020/07/26 | Published: 2020/10/29 | ePublished: 2020/10/29
Received: 2020/04/24 | Accepted: 2020/07/26 | Published: 2020/10/29 | ePublished: 2020/10/29
References
1. ISIRI (Institute of Standards and Industrial Research of Iran). Gharahghorut-Specifications and test methods. 2011;No:13299(ICS:67.100.99). [Google Scholar]
2. Smith JP, Daifas DP, El-Khoury W, Koukoutsis J, El-Khoury A. Shelf life and safety concerns of bakery products-a review. Crit Rev Food Sci Nutr. 2004; 44(1): 19-55. doi: 10.1080/10408690490263774. [DOI:10.1080/10408690490263774] [PubMed] [Google Scholar]
3. ISIRI (Institute of Standards and Industrial Research of Iran). Microbiological of pastry and confectionary products-specifications and test method. 2019;No:2395:5-11.
4. ISIRI (Institute of Standards and Industrial Research of Iran). Microbiology of food and animal feeding stuffs-Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 1: Detection and enumeration by MPN technique with per-enrichment. 2007;No: 2461:1.
5. ISIRI (Institute of Standards and Industrial Research of Iran). Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of coagulase- positive Staphylococci (Staphylococcus aureus and other species) - Part 3: Detection and MPN technique for low numbers. 2006;No: 6806-3.
6. ISIRI (Institute of Standards and Industrial Research of Iran). Microbiology of food and animal feeding stuffs -Detection and enumeration of presumptive Escherichia coli -Most probable number technique. 2005;No: 2946(ICS:30.100.07).
7. ISIRI (Institute of Standards and Industrial Research of Iran). Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of yeasts and molds - Part 2: Colony count technique in products with water activity less than or equal to 0.9. 2008;No: 10899-.
8. ISIRI (Institute of Standards and Industrial Research of Iran). Packaged agricultural products used in food industry- Sampling. 2018;No: 2836(ICS: 67.040).
9. Mohammadi A, Hashemi M, Hosseini SM. Comparison of antifungal activities of various essential oils on the Phytophthora drechsleri, the causal agent of fruit decay. Iran J Microbiol. 2015; 7(1): 31-7. [PubMed] [Google Scholar]
10. ISIRI (Institute of Standards and Industrial Research of Iran). Microbiology of the food chain- Horizontal method for the detectionand enumeration of Enterobacteriaceae. Part 2: Colony-count technique. 2018;No: 2461-2(ICS: 07.100.01). [Google Scholar]
11. ISIRI (Institute of Standards and Industrial Research of Iran). Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of coliforms - Most probable number technique. 2010;No: 11166(ICS: 07.100.30). [Google Scholar]
12. ISIRI (Institute of Standards and Industrial Research of Iran). Microbiology of the food chain-Horizontal method for the detectionand enumeration of EnterobacteriaceaePart 1: Detection of Enterobacteriaceae. 2018;No: 2461-1(ICS: 07.100.30). [Google Scholar]
13. Khavas Z, Mohammadi A, Hosseini SM, Fakhari J. Evaluation of Microbial Contamination of Sohan Produced in Qom, Iran, with Reference to National Standards. Arch Hyg Sci. 2019; 8(3): 172-7. [DOI:10.29252/ArchHygSci.8.3.172] [Google Scholar]
14. Mohammadi A, Hashemi M, Hosseini SM, Hosseini M. Antimicrobial Activity of Essential Oils of Cinnamomum zeylanicum, Mentha piperita, Zataria multiflora Boiss and Thymus vulgaris Against Pathogenic Bacteria. Med Lab J. 2016; 10(2): 32-40. DOI: 10.18869/acadpub.mlj.10.2.32. [DOI:10.18869/acadpub.mlj.10.2.32] [Google Scholar]
15. ISIRI (Institute of Standards and Industrial Research of Iran). Milk and milk products - Determination of nitrogen content -Part 1:Kjeldahl principle and crude protein calculation. 2016;No: 9188-1(ICS: 67.100.10). [Google Scholar]
16. ISIRI (Institute of Standards and Industrial Research of Iran). Determination of fat content of cheese and melted cheese. No: 760. [Google Scholar]
17. ISIRI (Institute of Standards and Industrial Research of Iran). Determination of the ash content of processed cheese. No: 1755.
18. ISIRI (Institute of Standards and Industrial Research of Iran). Cheese and processed cheese productsDetermination of totall phosphorus content- Molecular absorption spectrometric method. 2013;No: 1808(ICS: 67.100.30). [Google Scholar]
19. Beig Mohammadi M, Bolandi M, Ghodusi HB. Production and physicochemical, rheological, sensory analysis of "Lour" cheese. Food Sci Technol. 2015; 12(49): 41-9. [Google Scholar]
20. Tajizadegan H, Doosti; A, Nasr D, Narges S. Health Quality Assessment and Microbial Contamination (Salmonella, Kelly Form, Escherichia Kelly and Staphylococcus aureus) in Kashk and Qara Qorut Traditional Chaharmahal and Bakhtiari Province. In: 15th Iranian Veterinary Congress, Tehran, Iranian Veterinary Society, [Internet]. 1387. Available from: https://www.civilica.com/Paper-THVC15-THVC15_703.html [Google Scholar]
21. Frazier WC, Westhoff DC. Food Microbiology. 18th ed. McGrawHill. India; 2003.
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