Jorjani Biomedicine Journal
فصلنامه علمی پژوهشی زیست پزشکی جرجانی
Jorjani Biomed J
Medical Sciences
http://goums.ac.ir/jorjanijournal
1
admin
2645-3509
10.52547/jorjanibiomedj
en
jalali
1398
4
1
gregorian
2019
7
1
7
2
online
1
fulltext
en
Increased expression of PD-1 on CD4+ T cells subsets in Rheumatoid Arthritis patients
علوم پایه پزشکی
Basic Medical Sciences
تحقیقی
Original article
<strong>Study objectives:</strong> Rheumatoid arthritis (RA) is a common chronic and systemic autoimmune disease, characterized by inflammation and the destruction of the joints. It is well known that CD4+ T cells play a major role in the pathogenesis of RA. Expanded subpopulations of CD4+ T cells have been reported in RA patients. Here, we investigated the expression of PD-1 on subsets of CD4+ T cells (CD4+CD28- and CD4+CD28+ T cells) in the peripheral blood (PB) and synovial fluid (SF) of patients with RA.<br>
<strong>Methods: </strong>A total of 42 RA patients, including 10 newly diagnosed (ND) and 32 relapsed (RL) cases and also 20 healthy controls were enrolled. Phenotypic characterization subsets of CD4+ T cells were evaluated by flow cytometry, using fluorescence conjugated specific human monoclonal antibodies.<br>
<strong>Results:</strong> The frequency of CD4+CD28+ T cells was significantly increased in SF versus PB in ND and RL patients. In contrast, the percentage of CD4+CD28- T cells was elevated in PB of ND and RL patients comparison to SF. Expression of PD-1 on CD4+CD28+ and CD4+CD28- T cells in PB of ND and RL patients was significantly higher than the healthy controls. Furthermore, PD-1 expression on CD4+CD28+ and CD4+CD28- T cells in SF versus PB of RL patients were significant increased.<br>
<strong>Conclusion: </strong>These data suggest that CD4+ T cells subsets in RA patients were resistance to PD-1 mediated effects and PD-1 has insufficient ability to suppression of CD4+T cells.
CD4+CD28+ T cells,CD4+CD28- T cells,CXCR3,PD-1,Rheumatoid Arthritis
61
70
http://goums.ac.ir/jorjanijournal/browse.php?a_code=A-10-551-1&slc_lang=en&sid=1
faezeh
ajam
faezeh_ajam@yahoo.com
10031947532846005317
10031947532846005317
No
Student Research Committee, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
Mehrdad
Aghaei
mehrdadaghaie@yahoo.com
10031947532846005318
10031947532846005318
No
Golestan Rheumatology Research Center (GRRC), Golestan University of Medical Sciences, Gorgan, Iran
Saeed
Mohammadi
s.mohammadi@goums.ac.ir
10031947532846005319
10031947532846005319
No
Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Mohsen
Saeedi
saeedi.m50@gmail.com
10031947532846005320
10031947532846005320
No
Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Nasser
Behnampour
behnampour@yahoo.com
10031947532846005321
10031947532846005321
No
Public Health Department, Faculty of Health, Golestan University of Medical Sciences, Gorgan, Iran
Ali
Memarian
alimemarian@goums.ac.ir
10031947532846005322
10031947532846005322
Yes
Golestan Rheumatology Research Center (GRRC), Golestan University of Medical Sciences, Gorgan, IranGolestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran