Volume 8, Issue 1 (3-2020)                   Jorjani Biomed J 2020, 8(1): 51-59 | Back to browse issues page


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Kaikhosravi F, Daryanoosh F, Koushkie Jahromi M, Nemati J. The Effect of High Intensity Interval Training with Genistein on Biomechanical Properties of Femur Bone in Elderly Female Rats. Jorjani Biomed J. 2020; 8 (1) :51-59
URL: http://goums.ac.ir/jorjanijournal/article-1-713-en.html
1- Department of Sport Sciences, Shiraz University, Iran
2- Department of Sport Sciences, Shiraz University, Iran , daryanoosh@shirazu.ac.ir
Abstract:   (1290 Views)
Background and objective: Proper exercise and nutrition can help prevent bone disorders in old age, therefore present study aimed to investigate the effects of high intensity interval training (HIIT) with genistein (Ge) on biomechanical properties of femur bone in elderly female rats.
Material and Methods: In this experimental study, 40 elderly female rats with mean age of 18- 24 months and mean weight of 220.15±15.28 g were divided into five groups of eight rats including: 1) control (C), 2) sham (Sh), 3) HIIT, 4) HIIT + Ge, and 5) Ge. During eight weeks groups 3 and 4 performed HIIT for three sessions per week with an intensity of 90 to 95% of maximum oxygen consumption (VO2max) in high intensity intervals and 40 to 45% VO2max in low intensity intervals and groups 4 and 5 received 60 mg/kg/day Ge peritoneally. Maximum bending strength (Fmax) measured with three- point bending test and serum levels of calcium (Ca) and phosphorus were measured by o-Cresolphthalein and molybdate methods, respectively. Shapiro- Wilk, one way ANOVA with Tukey’s post- hoc tests were used for analysis of data (P≤0.05).
Results: HIIT (P=0.02), Ge (P=0.001) and HIIT+Ge (P=0.001) significantly increased Fmax also Ge (P=0.04) and HIIT+Ge (P=0.03) had more favorable effect on increasing Fmax compare to HIIT nevertheless HIIT, Ge and HIIT+Ge had not significant effect on Ca and phosphorus (P≥0.05).
Conclusion: Although HIIT and Ge alone can enhance Fmax in femur of elderly female rats, nevertheless HIIT+Ge has more favorable effect on increase of Fmax compare to HIIT.
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Type of Article: Original article | Subject: Health
Received: 2020/01/22 | Accepted: 2020/03/1 | Published: 2020/03/1

References
1. Tadayon Najafabadi M, Shalikar Z, Adedi P, Bamshad Z. The impact of omega-3 fatty acids on depression of menopausal women: a randomized double-blind clinical trial. J Arak Univ Med Sci. 2013;16(1):16-23. [view at publisher] [Google Scholar]
2. Dubnov-Raz G, Pines A, Berry EM. Diet and lifestyle in managing postmenopausal obesity. Climacteric. 2007;10(sup2):38-41. [view at publisher] [DOI] [Google Scholar]
3. Zar A, Hosseini SA, Homaion A. Effect of eight-week aquagymnastic training on liver enzymes and lipid profile of middle-aged women. 2016; [Google Scholar]
4. Iranmanesh S, Vahdati A, Afrouz T. Comparison of the effects of ethinyl estradiol and genistein on serum lipids and lipoproteins of hypercholestrolemic male rats. Physiol Pharmacol. 2006;10(2):159-64. [Google Scholar]
5. Miao Q, Li J-G, Miao S, Hu N, Zhang J, Zhang S, et al. The bone-protective effect of genistein in the animal model of bilateral ovariectomy: roles of phytoestrogens and PTH/PTHR1 against post-menopausal osteoporosis. Int J Mol Sci. 2012;13(1):56-70. [view at publisher] [DOI] [Google Scholar]
6. Chi XX, Chu XL, Zhang T, Cao LK. Effect of genistein on the gene expressions of androgen generating key enzymes StAR, P450scc and CYP19 in rat ovary. Pol J Vet Sci. 2019;279-86. [Google Scholar]
7. Sehmisch S, Hammer F, Christoffel J, Seidlova-Wuttke D, Tezval M, Wuttke W, et al. Using the biomechanical properties of bone to compare Genistein, Resveratrol and 8-Prenylaringenin as agent for preventing osteoporosis. Planta Med. 2008;74:794-801. [DOI] [Google Scholar]
8. Rezaie M, Azarbayjani MA, Peeri M, Hosseini SA. The Effect of Exercise, Ozone, and Mesenchymal Stem Cells Therapy on CB-1 and GABA Gene Expression in the Cartilage Tissue of Rats With Knee Osteoarthritis. Pharm Biomed Res. 2020;6(1):45-52. [DOI] [Google Scholar]
9. Haghjoo M, Azarbayjani MA, Peeri M, Hosseini SA. Effect of Training, Hyaluronic Acid, and Mesenchymal Stem Cell Therapies on Osteocalcin Gene Expression in Cartilage Tissue of Rats with Knee Osteoarthritis. Gene, Cell Tissue. 2019;6(4). [DOI] [Google Scholar]
10. Moreira LDF, Oliveira ML de, Lirani-Galvão AP, Marin-Mio RV, Santos RN dos, Lazaretti-Castro M. Physical exercise and osteoporosis: effects of different types of exercises on bone and physical function of postmenopausal women. Arq Bras Endocrinol Metabol. 2014;58(5):514-22. [view at publisher] [DOI] [Google Scholar]
11. Abbasi T, Nazarali P, Hedayati M, Alizadeh R. The effect of eight weeks of high intensity interval training on osteoponetin and some bone mineral indices in young women. J Phys Educ Sport. 2018;18:532-5. [Google Scholar]
12. GardashiAfousi A, Khashayar P, Gaeini A, Choubineh S, Fallahi AS. Effect high intensity interval training on hormonal factors influence on bone metabolism. J Med Scie Razi. 2015;22(130):31-7. [Google Scholar]
13. Li F-H, Sun L, Zhu M, Li T, Gao H-E, Wu D-S, et al. Beneficial alterations in body composition, physical performance, oxidative stress, inflammatory markers, and adipocytokines induced by long-term high-intensity interval training in an aged rat model. Exp Gerontol. 2018;113:150-62. [view at publisher] [DOI] [Google Scholar]
14. Peacock M. Phosphate Metabolism in Health and Disease. Calcif Tissue Int. 2020;1-13. [DOI] [Google Scholar]
15. Eliaz N, Metoki N. Calcium phosphate bioceramics: a review of their history, structure, properties, coating technologies and biomedical applications. Materials (Basel). 2017;10(4):334. [view at publisher] [DOI] [Google Scholar]
16. Friedman MA, Bailey AM, Rondon MJ, McNerny EM, Sahar ND, Kohn DH. Calcium-and phosphorus-supplemented diet increases bone mass after short-term exercise and increases bone mass and structural strength after long-term exercise in adult mice. PLoS One. 2016;11(3):e0151995. [view at publisher] [DOI] [Google Scholar]
17. Charoenphandhu N. Physical activity and exercise affect intestinal calcium absorption: a perspective review. Sport Med. 2007;7(1):171-81. [Google Scholar]
18. Scott JPR, Sale C, Greeves JP, Casey A, Dutton J, Fraser WD. Treadmill running reduces parathyroid hormone concentrations during recovery compared with a nonexercising control group. J Clin Endocrinol Metab. 2014;99(5):1774-82. [view at publisher] [DOI] [Google Scholar]
19. Smith JK, Dykes R, Chi DS. The effect of long-term exercise on the production of osteoclastogenic and antiosteoclastogenic cytokines by peripheral blood mononuclear cells and on serum markers of bone metabolism. J Osteoporos. 2016;2016. [view at publisher] [DOI] [Google Scholar]
20. Bitto A, Burnett BP, Polito F, Levy RM, Marini H, Stefano V Di, et al. Genistein aglycone reverses glucocorticoid‐induced osteoporosis and increases bone breaking strength in rats: a comparative study with alendronate. Br J Pharmacol. 2009;156(8):1287-95. [view at publisher] [DOI] [Google Scholar]
21. Marini H, Minutoli L, Polito F, Bitto A, Altavilla D, Atteritano M, et al. OPG and sRANKL serum concentrations in osteopenic, postmenopausal women after 2‐year genistein administration. J Bone Miner Res. 2008;23(5):715-20. [view at publisher] [DOI] [Google Scholar]
22. Li Y, Xing X, Wang H, Weng X, Yu S, Dong G. Dose-dependent effects of genistein on bone homeostasis in rats' mandibular subchondral bone. Acta Pharmacol Sin. 2012;33(1):66-74. [DOI] [Google Scholar]
23. Hafez Dalia A. Anti-Osteoporotic Activity of Soy Total Extract and Genistein Compound in Ovariectomized RatsNo Title. J Am Sci. 2012;8(8):698-703.
24. Wu J, Wang XX, Takasaki M, Ohta A, Higuchi M, Ishimi Y. Cooperative effects of exercise training and genistein administration on bone mass in ovariectomized mice. J Bone Miner Res. 2001;16(10):1829-36. [view at publisher] [DOI] [Google Scholar]
25. Hellings A, Buchan L, Castro M, St. Aubin CR, Fisher AL, Al-Nakkash L, et al. Bone Strength Is Improved with Genistein Treatment in Mice with Diet-Induced Obesity. Curr Dev Nutr. 2019;3(11):nzz121. [view at publisher] [DOI] [Google Scholar]
26. Nakajima D, Kim C-S, Oh T-W, Yang C-Y, Naka T, Igawa S, et al. Suppressive effects of genistein dosage and resistance exercise on bone loss in ovariectomized rats. J Physiol Anthropol Appl Human Sci. 2001;20(5):285-91. [DOI] [Google Scholar]

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